Biotechnological production and current feasible applications of neokestose: A review
Neokestose is a trisaccharide that consists of a fructosyl unit linked to the glucose moiety of a sucrose acceptor by β-(2,6) glycosidic linkage. It naturally occurs at low levels in some plants as opposed to 1-kestose and inulin-fructooligosaccharides, which are plentiful in chicory root and Jerusa...
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| Format: | Article |
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001367 |
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| author | Nalapat Leangnim Lalida Shank Kullapa Chanawanno Chartchai Khanongnuch Apinun Kanpiengjai |
| author_facet | Nalapat Leangnim Lalida Shank Kullapa Chanawanno Chartchai Khanongnuch Apinun Kanpiengjai |
| author_sort | Nalapat Leangnim |
| collection | DOAJ |
| description | Neokestose is a trisaccharide that consists of a fructosyl unit linked to the glucose moiety of a sucrose acceptor by β-(2,6) glycosidic linkage. It naturally occurs at low levels in some plants as opposed to 1-kestose and inulin-fructooligosaccharides, which are plentiful in chicory root and Jerusalem artichokes. Production of neokestose is limited due to its availability, while expanded applications require further research development to elucidate its other potential properties. In this review, natural sources of neokestose and biotechnological approaches for neokestose production, including fermentation, microbial synthesis, and enzymatic synthesis, have been summarized and discussed to explore the usefulness and applicability of each production approach. The key enzymes associated with neokestose synthesis are 6G-fructan:fructan fructosyltransferase and 6G-fructofuranosidase. Their different catalytic mechanisms, substrate specificity, and natural sources have also been summarized and discussed in this review. Thus far, there have only been a few reports on the physicochemical and biological properties of neokestose. Preliminary investigations on the biological properties of neokestose, such as its prebiotic and chemopreventive properties, would likely gain considerable attention and lead to the discovery of new beneficial properties of neokestose. These outcomes will likely lead to promising new means of neokestose utilization across various industrial applications. |
| format | Article |
| id | doaj-art-61ce35dcbb6640119ebf1ab6caa9bcc8 |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-61ce35dcbb6640119ebf1ab6caa9bcc82025-08-20T03:20:58ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010079810.1016/j.carpta.2025.100798Biotechnological production and current feasible applications of neokestose: A reviewNalapat Leangnim0Lalida Shank1Kullapa Chanawanno2Chartchai Khanongnuch3Apinun Kanpiengjai4Department of Chemistry, Faculty of Science, Chiang Mai University, Chaing Mai, 50200, Thailand; Office of Research Administration, Chiang Mai University, Chiang Mai, 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chaing Mai, 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chaing Mai, 50200, ThailandResearch Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chaing Mai, 50200, Thailand; Corresponding author.Neokestose is a trisaccharide that consists of a fructosyl unit linked to the glucose moiety of a sucrose acceptor by β-(2,6) glycosidic linkage. It naturally occurs at low levels in some plants as opposed to 1-kestose and inulin-fructooligosaccharides, which are plentiful in chicory root and Jerusalem artichokes. Production of neokestose is limited due to its availability, while expanded applications require further research development to elucidate its other potential properties. In this review, natural sources of neokestose and biotechnological approaches for neokestose production, including fermentation, microbial synthesis, and enzymatic synthesis, have been summarized and discussed to explore the usefulness and applicability of each production approach. The key enzymes associated with neokestose synthesis are 6G-fructan:fructan fructosyltransferase and 6G-fructofuranosidase. Their different catalytic mechanisms, substrate specificity, and natural sources have also been summarized and discussed in this review. Thus far, there have only been a few reports on the physicochemical and biological properties of neokestose. Preliminary investigations on the biological properties of neokestose, such as its prebiotic and chemopreventive properties, would likely gain considerable attention and lead to the discovery of new beneficial properties of neokestose. These outcomes will likely lead to promising new means of neokestose utilization across various industrial applications.http://www.sciencedirect.com/science/article/pii/S2666893925001367NeokestoseNeofructooligosaccharidesPrebiotic6G-fructofuranosidase6G-fructosyltransferase |
| spellingShingle | Nalapat Leangnim Lalida Shank Kullapa Chanawanno Chartchai Khanongnuch Apinun Kanpiengjai Biotechnological production and current feasible applications of neokestose: A review Carbohydrate Polymer Technologies and Applications Neokestose Neofructooligosaccharides Prebiotic 6G-fructofuranosidase 6G-fructosyltransferase |
| title | Biotechnological production and current feasible applications of neokestose: A review |
| title_full | Biotechnological production and current feasible applications of neokestose: A review |
| title_fullStr | Biotechnological production and current feasible applications of neokestose: A review |
| title_full_unstemmed | Biotechnological production and current feasible applications of neokestose: A review |
| title_short | Biotechnological production and current feasible applications of neokestose: A review |
| title_sort | biotechnological production and current feasible applications of neokestose a review |
| topic | Neokestose Neofructooligosaccharides Prebiotic 6G-fructofuranosidase 6G-fructosyltransferase |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001367 |
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