The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling Pathway
Isaridin E, a cyclodepsipeptide derived from the marine fungus <i>Beauveria felina</i> (SYSU-MS7908), has been demonstrated to possess multiple biological properties. In this study, we employed both lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs) and a...
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| Format: | Article |
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MDPI AG
2025-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/23/4/145 |
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| author | Jing Liu Xin Zeng Yu-Quan Lin Yu-Sheng Peng Lan Liu Sen-Hua Chen Yan-Hua Du |
| author_facet | Jing Liu Xin Zeng Yu-Quan Lin Yu-Sheng Peng Lan Liu Sen-Hua Chen Yan-Hua Du |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Isaridin E, a cyclodepsipeptide derived from the marine fungus <i>Beauveria felina</i> (SYSU-MS7908), has been demonstrated to possess multiple biological properties. In this study, we employed both lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs) and a LPS-induced murine endotoxemia model to investigate its anti-inflammatory effects. Our results revealed that isaridin E suppressed the expression of pro-inflammatory cytokines and adhesion molecules in a concentration dependent manner, while also reducing monocyte adhesion to endothelial cells. Furthermore, this compound attenuated vascular hyperpermeability and inflammatory cell infiltration in the lungs, as well as preserving the integrity of the aortic and pulmonary tissues. At the molecular level, isaridin E was found to downregulate TLR4 expression, increase IκBα levels, and inhibit the LPS-induced phosphorylation and nuclear translocation of NF-κB p65. In conclusion, our findings indicate that isaridin E exerts robust anti-inflammatory effects in LPS-induced endotoxemia through the suppression of the TLR4/NF-κB signaling axis, positioning it as a promising therapeutic candidate for vascular inflammatory disorders. |
| format | Article |
| id | doaj-art-61ca6862d34e45f98d7e0ba5afaf9f62 |
| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-61ca6862d34e45f98d7e0ba5afaf9f622025-08-20T02:18:19ZengMDPI AGMarine Drugs1660-33972025-03-0123414510.3390/md23040145The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling PathwayJing Liu0Xin Zeng1Yu-Quan Lin2Yu-Sheng Peng3Lan Liu4Sen-Hua Chen5Yan-Hua Du6Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaSchool of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaSchool of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaDepartment of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaIsaridin E, a cyclodepsipeptide derived from the marine fungus <i>Beauveria felina</i> (SYSU-MS7908), has been demonstrated to possess multiple biological properties. In this study, we employed both lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs) and a LPS-induced murine endotoxemia model to investigate its anti-inflammatory effects. Our results revealed that isaridin E suppressed the expression of pro-inflammatory cytokines and adhesion molecules in a concentration dependent manner, while also reducing monocyte adhesion to endothelial cells. Furthermore, this compound attenuated vascular hyperpermeability and inflammatory cell infiltration in the lungs, as well as preserving the integrity of the aortic and pulmonary tissues. At the molecular level, isaridin E was found to downregulate TLR4 expression, increase IκBα levels, and inhibit the LPS-induced phosphorylation and nuclear translocation of NF-κB p65. In conclusion, our findings indicate that isaridin E exerts robust anti-inflammatory effects in LPS-induced endotoxemia through the suppression of the TLR4/NF-κB signaling axis, positioning it as a promising therapeutic candidate for vascular inflammatory disorders.https://www.mdpi.com/1660-3397/23/4/145isaridin Emarine natural productsendothelial inflammationTLR4NF-κB |
| spellingShingle | Jing Liu Xin Zeng Yu-Quan Lin Yu-Sheng Peng Lan Liu Sen-Hua Chen Yan-Hua Du The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling Pathway Marine Drugs isaridin E marine natural products endothelial inflammation TLR4 NF-κB |
| title | The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling Pathway |
| title_full | The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling Pathway |
| title_fullStr | The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling Pathway |
| title_full_unstemmed | The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling Pathway |
| title_short | The Marine Compound Isaridin E Ameliorates Lipopolysaccharide-Induced Vascular Endothelial Inflammation via the Downregulation of the TLR4/NF-κB Signaling Pathway |
| title_sort | marine compound isaridin e ameliorates lipopolysaccharide induced vascular endothelial inflammation via the downregulation of the tlr4 nf κb signaling pathway |
| topic | isaridin E marine natural products endothelial inflammation TLR4 NF-κB |
| url | https://www.mdpi.com/1660-3397/23/4/145 |
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