Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice
Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/8391253 |
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| _version_ | 1849411155570720768 |
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| author | Haojun Zhang Tingting Zhao Zhiguo Li Meihua Yan Hailing Zhao Bin Zhu Ping Li |
| author_facet | Haojun Zhang Tingting Zhao Zhiguo Li Meihua Yan Hailing Zhao Bin Zhu Ping Li |
| author_sort | Haojun Zhang |
| collection | DOAJ |
| description | Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous type 2 diabetic db/db mice by using the cDNA microarray. Compared with control db/m mice, the db/db mice exhibited marked increases in body weight, kidney weight, and urinary albumin excretion. Renal histological analysis revealed mesangial expansion and thickness of the basement membrane in the kidney of the db/db mice. A total of 355 differentially expressed genes (DEGs) were identified by microarray analysis. Pathway enrichment analysis suggested that biological oxidation, bile acid metabolism, and steroid hormone synthesis were the 3 major significant pathways. The top 10 hub genes were selected from the constructed PPI network of DEGs, including Ccnb2 and Nr1i2, which remained largely unclear in DN. We believe that our study can help elucidate the molecular mechanisms underlying DN. |
| format | Article |
| id | doaj-art-61bd7cf3babd4b88b6ffd993448f9921 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-61bd7cf3babd4b88b6ffd993448f99212025-08-20T03:34:52ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/83912538391253Transcriptional Profile of Kidney from Type 2 Diabetic db/db MiceHaojun Zhang0Tingting Zhao1Zhiguo Li2Meihua Yan3Hailing Zhao4Bin Zhu5Ping Li6Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaDepartment of Medical Research Center, International Science and Technology Cooperation Base of Geriatric Medicine, North China University of Science and Technology, Tangshan, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaDiabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous type 2 diabetic db/db mice by using the cDNA microarray. Compared with control db/m mice, the db/db mice exhibited marked increases in body weight, kidney weight, and urinary albumin excretion. Renal histological analysis revealed mesangial expansion and thickness of the basement membrane in the kidney of the db/db mice. A total of 355 differentially expressed genes (DEGs) were identified by microarray analysis. Pathway enrichment analysis suggested that biological oxidation, bile acid metabolism, and steroid hormone synthesis were the 3 major significant pathways. The top 10 hub genes were selected from the constructed PPI network of DEGs, including Ccnb2 and Nr1i2, which remained largely unclear in DN. We believe that our study can help elucidate the molecular mechanisms underlying DN.http://dx.doi.org/10.1155/2017/8391253 |
| spellingShingle | Haojun Zhang Tingting Zhao Zhiguo Li Meihua Yan Hailing Zhao Bin Zhu Ping Li Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice Journal of Diabetes Research |
| title | Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice |
| title_full | Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice |
| title_fullStr | Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice |
| title_full_unstemmed | Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice |
| title_short | Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice |
| title_sort | transcriptional profile of kidney from type 2 diabetic db db mice |
| url | http://dx.doi.org/10.1155/2017/8391253 |
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