Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction
Aflatoxins harm the reproductive system and gamete development in animals. Primordial germ cells (PGCs) in chickens, as ancestral cells of gametes, are essential for genetic transmission, yet the impact and mechanisms of aflatoxins on them remain elusive. This study systematically investigated the e...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000636 |
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| author | Ying-Jie Niu Jun Wu Wenjie Ren Guangzheng Liu Gaoyuan Wu Yixiu Peng Dan Zheng Kai Jin Qisheng Zuo Guohui Li Wei Han Xiang-Shun Cui Guohong Chen Bichun Li |
| author_facet | Ying-Jie Niu Jun Wu Wenjie Ren Guangzheng Liu Gaoyuan Wu Yixiu Peng Dan Zheng Kai Jin Qisheng Zuo Guohui Li Wei Han Xiang-Shun Cui Guohong Chen Bichun Li |
| author_sort | Ying-Jie Niu |
| collection | DOAJ |
| description | Aflatoxins harm the reproductive system and gamete development in animals. Primordial germ cells (PGCs) in chickens, as ancestral cells of gametes, are essential for genetic transmission, yet the impact and mechanisms of aflatoxins on them remain elusive. This study systematically investigated the effects of aflatoxin B1 (AFB1) on chicken PGCs and their potential mechanisms using an in vitro culture model. We observed a significant reduction in PGC numbers and an increase in apoptosis levels with AFB1 treatment. Further analysis revealed that AFB1 induced mitochondrial structural and functional abnormalities. Additionally, AFB1 treatment led to increased oxidative stress, lipid peroxidation, ferroptosis, and autophagy in chicken PGCs, ultimately affecting their biological characteristics. Interestingly, we found that the NRF2-mediated antioxidant pathway was activated in AFB1-treated PGCs. Inhibiting NRF2 exacerbated oxidative stress and cell death in PGCs, suggesting NRF2 upregulation plays a protective role under AFB1 regulation. This study illuminates AFB1's toxic effects on chicken PGCs and provides insights into potential mechanisms, establishing a basis for strategies to prevent and treat AFB1's adverse effects on poultry genetic transmission. |
| format | Article |
| id | doaj-art-61bd3c49c2f14ee2815af2334676371b |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-61bd3c49c2f14ee2815af2334676371b2025-02-12T05:30:06ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117727Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunctionYing-Jie Niu0Jun Wu1Wenjie Ren2Guangzheng Liu3Gaoyuan Wu4Yixiu Peng5Dan Zheng6Kai Jin7Qisheng Zuo8Guohui Li9Wei Han10Xiang-Shun Cui11Guohong Chen12Bichun Li13Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu 225125, ChinaPoultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu 225125, ChinaDepartment of Animal Science, Chungbuk National University, Cheongju, South KoreaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, China; Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China; Correspondence to: Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.Aflatoxins harm the reproductive system and gamete development in animals. Primordial germ cells (PGCs) in chickens, as ancestral cells of gametes, are essential for genetic transmission, yet the impact and mechanisms of aflatoxins on them remain elusive. This study systematically investigated the effects of aflatoxin B1 (AFB1) on chicken PGCs and their potential mechanisms using an in vitro culture model. We observed a significant reduction in PGC numbers and an increase in apoptosis levels with AFB1 treatment. Further analysis revealed that AFB1 induced mitochondrial structural and functional abnormalities. Additionally, AFB1 treatment led to increased oxidative stress, lipid peroxidation, ferroptosis, and autophagy in chicken PGCs, ultimately affecting their biological characteristics. Interestingly, we found that the NRF2-mediated antioxidant pathway was activated in AFB1-treated PGCs. Inhibiting NRF2 exacerbated oxidative stress and cell death in PGCs, suggesting NRF2 upregulation plays a protective role under AFB1 regulation. This study illuminates AFB1's toxic effects on chicken PGCs and provides insights into potential mechanisms, establishing a basis for strategies to prevent and treat AFB1's adverse effects on poultry genetic transmission.http://www.sciencedirect.com/science/article/pii/S0147651325000636Aflatoxin B1Primordial germ cellsOxidative stressMitochondrionNRF2 |
| spellingShingle | Ying-Jie Niu Jun Wu Wenjie Ren Guangzheng Liu Gaoyuan Wu Yixiu Peng Dan Zheng Kai Jin Qisheng Zuo Guohui Li Wei Han Xiang-Shun Cui Guohong Chen Bichun Li Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction Ecotoxicology and Environmental Safety Aflatoxin B1 Primordial germ cells Oxidative stress Mitochondrion NRF2 |
| title | Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction |
| title_full | Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction |
| title_fullStr | Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction |
| title_full_unstemmed | Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction |
| title_short | Aflatoxin B1 impairs the growth and development of chicken PGCs through oxidative stress and mitochondrial dysfunction |
| title_sort | aflatoxin b1 impairs the growth and development of chicken pgcs through oxidative stress and mitochondrial dysfunction |
| topic | Aflatoxin B1 Primordial germ cells Oxidative stress Mitochondrion NRF2 |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000636 |
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