Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia
This study was designed to investigate the prevalence of Salmonella and Shigella among outpatients in Jimma University Specialized Hospital, Southwest Ethiopia. Cross-sectional study was conducted involving a total of 176 outpatients. Stool specimens from both adult and pediatric outpatients were co...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2016/4210760 |
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| author | Tesfahun Lamboro Tsige Ketema Ketema Bacha |
| author_facet | Tesfahun Lamboro Tsige Ketema Ketema Bacha |
| author_sort | Tesfahun Lamboro |
| collection | DOAJ |
| description | This study was designed to investigate the prevalence of Salmonella and Shigella among outpatients in Jimma University Specialized Hospital, Southwest Ethiopia. Cross-sectional study was conducted involving a total of 176 outpatients. Stool specimens from both adult and pediatric outpatients were collected and analyzed for the presence of presumptive Salmonella and Shigella colonies followed by confirmation by biochemical tests. Pure cultures of Salmonella and Shigella species were further subjected to test for antibiotic resistance against the commonly used antibiotics. Furthermore, growth potential of the isolates in selected foods items was assessed following standard procedures. The result indicated that the prevalence of Salmonella and Shigella among outpatients in the study area was 19 (10.8%) and 2 (1.1%), respectively. In addition, Salmonella species were resistant to ampicillin (100%) followed by tetracycline (47.4%) and nalidixic acid (26.3%) while Shigella species were highly resistant to ampicillin and tetracycline (100%, each). Multidrug resistance towards maximum of four drugs was observed in both pathogens. The pathogens were observed growing to their infective dose within 24 hours. In conclusion, Salmonella and Shigella are still among microbes of public health importance in the study area. Thus, this calls for frequent monitory and evaluation of their prevalence and drug resistance patterns besides awareness development on water sanitation and hygienic food handling practices to the public at large. |
| format | Article |
| id | doaj-art-61b2ee8c453a4d2b9b152e3272f404b2 |
| institution | OA Journals |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-61b2ee8c453a4d2b9b152e3272f404b22025-08-20T02:35:22ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932016-01-01201610.1155/2016/42107604210760Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest EthiopiaTesfahun Lamboro0Tsige Ketema1Ketema Bacha2Department of Biology, College of Natural Sciences, Jimma University, P.O. Box 378, 1000 Jimma, EthiopiaDepartment of Biology, College of Natural Sciences, Jimma University, P.O. Box 378, 1000 Jimma, EthiopiaDepartment of Biology, College of Natural Sciences, Jimma University, P.O. Box 378, 1000 Jimma, EthiopiaThis study was designed to investigate the prevalence of Salmonella and Shigella among outpatients in Jimma University Specialized Hospital, Southwest Ethiopia. Cross-sectional study was conducted involving a total of 176 outpatients. Stool specimens from both adult and pediatric outpatients were collected and analyzed for the presence of presumptive Salmonella and Shigella colonies followed by confirmation by biochemical tests. Pure cultures of Salmonella and Shigella species were further subjected to test for antibiotic resistance against the commonly used antibiotics. Furthermore, growth potential of the isolates in selected foods items was assessed following standard procedures. The result indicated that the prevalence of Salmonella and Shigella among outpatients in the study area was 19 (10.8%) and 2 (1.1%), respectively. In addition, Salmonella species were resistant to ampicillin (100%) followed by tetracycline (47.4%) and nalidixic acid (26.3%) while Shigella species were highly resistant to ampicillin and tetracycline (100%, each). Multidrug resistance towards maximum of four drugs was observed in both pathogens. The pathogens were observed growing to their infective dose within 24 hours. In conclusion, Salmonella and Shigella are still among microbes of public health importance in the study area. Thus, this calls for frequent monitory and evaluation of their prevalence and drug resistance patterns besides awareness development on water sanitation and hygienic food handling practices to the public at large.http://dx.doi.org/10.1155/2016/4210760 |
| spellingShingle | Tesfahun Lamboro Tsige Ketema Ketema Bacha Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia |
| title_full | Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia |
| title_fullStr | Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia |
| title_full_unstemmed | Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia |
| title_short | Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia |
| title_sort | prevalence and antimicrobial resistance in salmonella and shigella species isolated from outpatients jimma university specialized hospital southwest ethiopia |
| url | http://dx.doi.org/10.1155/2016/4210760 |
| work_keys_str_mv | AT tesfahunlamboro prevalenceandantimicrobialresistanceinsalmonellaandshigellaspeciesisolatedfromoutpatientsjimmauniversityspecializedhospitalsouthwestethiopia AT tsigeketema prevalenceandantimicrobialresistanceinsalmonellaandshigellaspeciesisolatedfromoutpatientsjimmauniversityspecializedhospitalsouthwestethiopia AT ketemabacha prevalenceandantimicrobialresistanceinsalmonellaandshigellaspeciesisolatedfromoutpatientsjimmauniversityspecializedhospitalsouthwestethiopia |