Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies
The study evaluates different preparation methods for identifying the best strategy for extracting biologically active compounds from raw <i>Chamaenerion angustifolium</i> (L.) Holub plant material. The methodologies include direct aqueous methanol extraction with a combination of natura...
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/23/5530 |
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| author | Domantas Armonavičius Mantas Stankevičius Audrius Maruška |
| author_facet | Domantas Armonavičius Mantas Stankevičius Audrius Maruška |
| author_sort | Domantas Armonavičius |
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| description | The study evaluates different preparation methods for identifying the best strategy for extracting biologically active compounds from raw <i>Chamaenerion angustifolium</i> (L.) Holub plant material. The methodologies include direct aqueous methanol extraction with a combination of natural aerobic and anaerobic fermentation for 24–72 h, followed by 35 °C and 60 °C drying. Furthermore, the study also focuses on determining the different temperature storage conditions on the stability of biologically active compounds. UV-VIS spectroscopy was used to quantitatively evaluate the total content of phenolic compounds, flavonoids, and radical scavenging activity. For qualitative analysis, chromatographic separation with electrochemical detection (ED) of extracted compounds, a gradient high-performance liquid chromatography (HPLC) system was used. Study results indicate that 48 h natural aerobic fermentation followed by 35 °C drying and 75% (<i>v</i>/<i>v</i>) aqueous methanol extraction yielded the maximum amount of biologically active compounds in <i>Chamaenerion angustifolium</i> (L.) Holub leaves, blossom, and stem samples. Freezing samples in liquid nitrogen had the lowest impact on the total content of phenolic compounds, flavonoids, and radical scavenging activity. HPLC-ED system results identified chlorogenic acid, oenothein B, trans-p-Coumaric acid, ellagic acid, and rutin in <i>Chamaenerion angustifolium</i> (L.) Holub leave samples. |
| format | Article |
| id | doaj-art-61aab80c6ffc4630948628f150fe8633 |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-61aab80c6ffc4630948628f150fe86332025-08-20T01:55:41ZengMDPI AGMolecules1420-30492024-11-012923553010.3390/molecules29235530Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different StrategiesDomantas Armonavičius0Mantas Stankevičius1Audrius Maruška2Instrumental Analysis Open Access Centre, Institute of Research of Natural and Technological Sciences, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, LT-44404 Kaunas, LithuaniaInstrumental Analysis Open Access Centre, Institute of Research of Natural and Technological Sciences, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, LT-44404 Kaunas, LithuaniaInstrumental Analysis Open Access Centre, Institute of Research of Natural and Technological Sciences, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, LT-44404 Kaunas, LithuaniaThe study evaluates different preparation methods for identifying the best strategy for extracting biologically active compounds from raw <i>Chamaenerion angustifolium</i> (L.) Holub plant material. The methodologies include direct aqueous methanol extraction with a combination of natural aerobic and anaerobic fermentation for 24–72 h, followed by 35 °C and 60 °C drying. Furthermore, the study also focuses on determining the different temperature storage conditions on the stability of biologically active compounds. UV-VIS spectroscopy was used to quantitatively evaluate the total content of phenolic compounds, flavonoids, and radical scavenging activity. For qualitative analysis, chromatographic separation with electrochemical detection (ED) of extracted compounds, a gradient high-performance liquid chromatography (HPLC) system was used. Study results indicate that 48 h natural aerobic fermentation followed by 35 °C drying and 75% (<i>v</i>/<i>v</i>) aqueous methanol extraction yielded the maximum amount of biologically active compounds in <i>Chamaenerion angustifolium</i> (L.) Holub leaves, blossom, and stem samples. Freezing samples in liquid nitrogen had the lowest impact on the total content of phenolic compounds, flavonoids, and radical scavenging activity. HPLC-ED system results identified chlorogenic acid, oenothein B, trans-p-Coumaric acid, ellagic acid, and rutin in <i>Chamaenerion angustifolium</i> (L.) Holub leave samples.https://www.mdpi.com/1420-3049/29/23/5530biologically active compoundsextractionphenolic compoundsellagitanninsoenothein Bantioxidants |
| spellingShingle | Domantas Armonavičius Mantas Stankevičius Audrius Maruška Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies Molecules biologically active compounds extraction phenolic compounds ellagitannins oenothein B antioxidants |
| title | Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies |
| title_full | Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies |
| title_fullStr | Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies |
| title_full_unstemmed | Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies |
| title_short | Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies |
| title_sort | extraction of bioactive compounds and influence of storage conditions of raw material i chamaenerion angustifolium i l holub using different strategies |
| topic | biologically active compounds extraction phenolic compounds ellagitannins oenothein B antioxidants |
| url | https://www.mdpi.com/1420-3049/29/23/5530 |
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