Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material <i>Chamaenerion angustifolium</i> (L.) Holub Using Different Strategies
The study evaluates different preparation methods for identifying the best strategy for extracting biologically active compounds from raw <i>Chamaenerion angustifolium</i> (L.) Holub plant material. The methodologies include direct aqueous methanol extraction with a combination of natura...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/29/23/5530 |
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| Summary: | The study evaluates different preparation methods for identifying the best strategy for extracting biologically active compounds from raw <i>Chamaenerion angustifolium</i> (L.) Holub plant material. The methodologies include direct aqueous methanol extraction with a combination of natural aerobic and anaerobic fermentation for 24–72 h, followed by 35 °C and 60 °C drying. Furthermore, the study also focuses on determining the different temperature storage conditions on the stability of biologically active compounds. UV-VIS spectroscopy was used to quantitatively evaluate the total content of phenolic compounds, flavonoids, and radical scavenging activity. For qualitative analysis, chromatographic separation with electrochemical detection (ED) of extracted compounds, a gradient high-performance liquid chromatography (HPLC) system was used. Study results indicate that 48 h natural aerobic fermentation followed by 35 °C drying and 75% (<i>v</i>/<i>v</i>) aqueous methanol extraction yielded the maximum amount of biologically active compounds in <i>Chamaenerion angustifolium</i> (L.) Holub leaves, blossom, and stem samples. Freezing samples in liquid nitrogen had the lowest impact on the total content of phenolic compounds, flavonoids, and radical scavenging activity. HPLC-ED system results identified chlorogenic acid, oenothein B, trans-p-Coumaric acid, ellagic acid, and rutin in <i>Chamaenerion angustifolium</i> (L.) Holub leave samples. |
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| ISSN: | 1420-3049 |