Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines
Chronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment of inflammation can carry some risks. Therefore, research on herbal medicine that are suspected of having anti-inflammatory effects, such as Piper betle L., is important...
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University of Brawijaya
2023-06-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/528 |
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| author | Dinia Rizqi Dwijayanti Sapti Puspitarini Nashi Widodo |
| author_facet | Dinia Rizqi Dwijayanti Sapti Puspitarini Nashi Widodo |
| author_sort | Dinia Rizqi Dwijayanti |
| collection | DOAJ |
| description | Chronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment of inflammation can carry some risks. Therefore, research on herbal medicine that are suspected of having anti-inflammatory effects, such as Piper betle L., is important. This study aims to investigate the effect of P. betle L. extract on nitric oxide as a pro-inflammatory mediator. The dried leaves of P. betle L. were extracted by ethanol. RAW 264.7 cells were treated with LPS and P. betle L. extract (PBE). Nitric oxide was measured by the Griess method. Antioxidant activity was determined using 2,2–Diphenyl-1-picrylhydrazyl (DPPH) method. Total flavonoids and phenolic content were also identified by aluminium chloride and Folin–Ciocalteu colorimetric assay, respectively. This study demonstrated that the PBE has excellent NO suppression activity with the IC50 56.22±16.41 μg.mL-1, without cytotoxicity. PBE also has DPPH inhibitory concentration IC50 values of 279.67±11.36 ppm. Interestingly, PBE has a flavonoid content of 50.17±3.14 mg QE.g-1 and phenolic content of 128.92±1.2 mg GAE.g-1. These compounds are thought to be responsible for its anti-inflammatory as well as antioxidant. This study proved that P. betle L. leaves extract could be used as a candidate for anti-inflammatory drugs. Nevertheless, further research about the biological activity mechanism and their bioactive compounds' purification is still required. |
| format | Article |
| id | doaj-art-61a1dccc33d74ac98a295b33ffae9f02 |
| institution | Kabale University |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-61a1dccc33d74ac98a295b33ffae9f022025-08-20T03:53:39ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552023-06-011327883https://doi.org/10.21776/ub.jels.2023.013.02.02Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell LinesDinia Rizqi Dwijayanti0Sapti Puspitarini1Nashi Widodo2Universitas Brawijaya, Malang, IndonesiaState University of Surabaya, Surabaya, IndonesiaUniversitas Brawijaya, Malang, IndonesiaChronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment of inflammation can carry some risks. Therefore, research on herbal medicine that are suspected of having anti-inflammatory effects, such as Piper betle L., is important. This study aims to investigate the effect of P. betle L. extract on nitric oxide as a pro-inflammatory mediator. The dried leaves of P. betle L. were extracted by ethanol. RAW 264.7 cells were treated with LPS and P. betle L. extract (PBE). Nitric oxide was measured by the Griess method. Antioxidant activity was determined using 2,2–Diphenyl-1-picrylhydrazyl (DPPH) method. Total flavonoids and phenolic content were also identified by aluminium chloride and Folin–Ciocalteu colorimetric assay, respectively. This study demonstrated that the PBE has excellent NO suppression activity with the IC50 56.22±16.41 μg.mL-1, without cytotoxicity. PBE also has DPPH inhibitory concentration IC50 values of 279.67±11.36 ppm. Interestingly, PBE has a flavonoid content of 50.17±3.14 mg QE.g-1 and phenolic content of 128.92±1.2 mg GAE.g-1. These compounds are thought to be responsible for its anti-inflammatory as well as antioxidant. This study proved that P. betle L. leaves extract could be used as a candidate for anti-inflammatory drugs. Nevertheless, further research about the biological activity mechanism and their bioactive compounds' purification is still required.https://jels.ub.ac.id/index.php/jels/article/view/528inflammationlipopolysaccharidemacrophagenitric oxidepiper betle. |
| spellingShingle | Dinia Rizqi Dwijayanti Sapti Puspitarini Nashi Widodo Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines Journal of Experimental Life Science inflammation lipopolysaccharide macrophage nitric oxide piper betle. |
| title | Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines |
| title_full | Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines |
| title_fullStr | Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines |
| title_full_unstemmed | Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines |
| title_short | Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines |
| title_sort | piper betle l leaves extract potentially reduce the nitric oxide production on lps induced raw 264 7 cell lines |
| topic | inflammation lipopolysaccharide macrophage nitric oxide piper betle. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/528 |
| work_keys_str_mv | AT diniarizqidwijayanti piperbetlelleavesextractpotentiallyreducethenitricoxideproductiononlpsinducedraw2647celllines AT saptipuspitarini piperbetlelleavesextractpotentiallyreducethenitricoxideproductiononlpsinducedraw2647celllines AT nashiwidodo piperbetlelleavesextractpotentiallyreducethenitricoxideproductiononlpsinducedraw2647celllines |