Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of Mexico
Summary: Background: Acinetobacter baumannii is an opportunistic drug-resistant Gram-negative coccobacillus associated with nosocomial infections, representing a worldwide public health problem. Aim: The aim of this study was to analyse the dissemination of A. baumannii in two hospital buildings in...
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Elsevier
2025-03-01
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| Series: | Infection Prevention in Practice |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590088925000071 |
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| author | Samantha Villarreal-Cruz Adrián Camacho-Ortiz Samantha Flores-Treviño Licet Villarreal-Treviño Paola Bocanegra-Ibarias |
| author_facet | Samantha Villarreal-Cruz Adrián Camacho-Ortiz Samantha Flores-Treviño Licet Villarreal-Treviño Paola Bocanegra-Ibarias |
| author_sort | Samantha Villarreal-Cruz |
| collection | DOAJ |
| description | Summary: Background: Acinetobacter baumannii is an opportunistic drug-resistant Gram-negative coccobacillus associated with nosocomial infections, representing a worldwide public health problem. Aim: The aim of this study was to analyse the dissemination of A. baumannii in two hospital buildings in Mexico through phenotypic and genotypic characterization of clinical isolates obtained for three years. Methods: Clinical strains were collected from two buildings in a tertiary-care hospital in Monterrey, Mexico. After species identification by MALDI-TOF MS and PCR, antimicrobial susceptibility was determined by disk diffusion and microdilution methods, carbapenemase-encoding genes (OXA-23, -24, -51, and -58) were searched, and clonal diversity was analysed by PFGE and MLST. Findings: Among 204 specimens, 87.3% and 50.5% of the isolates were classified as multidrug-resistant (MDR) and difficult-to-treat-resistant (DTR), respectively. The OXA-24 gene was detected in 95% of the isolates. Most isolates (n=181) were grouped into 15 clones, four which predominated and disseminated after five months. Among ST detected (ST1694, ST758, ST124, and ST490), ST124, which belongs to the high-risk CC636 clonal complex, is reported for the first time in Mexico. Conclusions: Long-term persistence and dissemination of A. baumannii clones were observed in specific hospital wards from two buildings in a tertiary-care hospital in Mexico. High antimicrobial resistance, such as MDR and DTR, were observed in this hospital. DTR surveillance and early recognition of MDR A. baumannii clones should be performed routinely to prevent their dissemination. |
| format | Article |
| id | doaj-art-6179c612dbf3474ebee40dd477279480 |
| institution | Kabale University |
| issn | 2590-0889 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Infection Prevention in Practice |
| spelling | doaj-art-6179c612dbf3474ebee40dd4772794802025-02-10T04:34:46ZengElsevierInfection Prevention in Practice2590-08892025-03-0171100443Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of MexicoSamantha Villarreal-Cruz0Adrián Camacho-Ortiz1Samantha Flores-Treviño2Licet Villarreal-Treviño3Paola Bocanegra-Ibarias4Deparment of Microbiology, School of Biological Sciences, Autonomous University of Nuevo Leon, Av. Pedro de Alba S/N y Av. Manuel L. Barragán, Col. Cd Universitaria, C.P. 66450, San Nicolás de los Garza, Nuevo León, MexicoDepartment of Infectious Diseases, University Hospital “Dr. José E. González” and School of Medicine, Autonomous University of Nuevo Leon, Av. Francisco I. Madero Pte. S/N y Av. Gonzalitos. Col. Mitras Centro. 64460, Monterrey, Nuevo León, MexicoDepartment of Infectious Diseases, University Hospital “Dr. José E. González” and School of Medicine, Autonomous University of Nuevo Leon, Av. Francisco I. Madero Pte. S/N y Av. Gonzalitos. Col. Mitras Centro. 64460, Monterrey, Nuevo León, MexicoDeparment of Microbiology, School of Biological Sciences, Autonomous University of Nuevo Leon, Av. Pedro de Alba S/N y Av. Manuel L. Barragán, Col. Cd Universitaria, C.P. 66450, San Nicolás de los Garza, Nuevo León, MexicoDepartment of Infectious Diseases, University Hospital “Dr. José E. González” and School of Medicine, Autonomous University of Nuevo Leon, Av. Francisco I. Madero Pte. S/N y Av. Gonzalitos. Col. Mitras Centro. 64460, Monterrey, Nuevo León, Mexico; Corresponding author. Address: Hospital Universitario “Dr. José Eleuterio González”, Ave. Madero S/N esq Ave Gonzalitos, Mitras Centro, 66460, Monterrey, NL, Mexico. Tel.: +52 81 8389 1111ext2727.Summary: Background: Acinetobacter baumannii is an opportunistic drug-resistant Gram-negative coccobacillus associated with nosocomial infections, representing a worldwide public health problem. Aim: The aim of this study was to analyse the dissemination of A. baumannii in two hospital buildings in Mexico through phenotypic and genotypic characterization of clinical isolates obtained for three years. Methods: Clinical strains were collected from two buildings in a tertiary-care hospital in Monterrey, Mexico. After species identification by MALDI-TOF MS and PCR, antimicrobial susceptibility was determined by disk diffusion and microdilution methods, carbapenemase-encoding genes (OXA-23, -24, -51, and -58) were searched, and clonal diversity was analysed by PFGE and MLST. Findings: Among 204 specimens, 87.3% and 50.5% of the isolates were classified as multidrug-resistant (MDR) and difficult-to-treat-resistant (DTR), respectively. The OXA-24 gene was detected in 95% of the isolates. Most isolates (n=181) were grouped into 15 clones, four which predominated and disseminated after five months. Among ST detected (ST1694, ST758, ST124, and ST490), ST124, which belongs to the high-risk CC636 clonal complex, is reported for the first time in Mexico. Conclusions: Long-term persistence and dissemination of A. baumannii clones were observed in specific hospital wards from two buildings in a tertiary-care hospital in Mexico. High antimicrobial resistance, such as MDR and DTR, were observed in this hospital. DTR surveillance and early recognition of MDR A. baumannii clones should be performed routinely to prevent their dissemination.http://www.sciencedirect.com/science/article/pii/S2590088925000071AcinetobacterDisseminationCarbapenemasesOXA-24Difficult-to-treat resistant |
| spellingShingle | Samantha Villarreal-Cruz Adrián Camacho-Ortiz Samantha Flores-Treviño Licet Villarreal-Treviño Paola Bocanegra-Ibarias Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of Mexico Infection Prevention in Practice Acinetobacter Dissemination Carbapenemases OXA-24 Difficult-to-treat resistant |
| title | Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of Mexico |
| title_full | Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of Mexico |
| title_fullStr | Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of Mexico |
| title_full_unstemmed | Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of Mexico |
| title_short | Intrahospital dissemination of multidrug-resistant Acinetobacter baumannii at a teaching hospital in Northeast of Mexico |
| title_sort | intrahospital dissemination of multidrug resistant acinetobacter baumannii at a teaching hospital in northeast of mexico |
| topic | Acinetobacter Dissemination Carbapenemases OXA-24 Difficult-to-treat resistant |
| url | http://www.sciencedirect.com/science/article/pii/S2590088925000071 |
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