Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico
Introduction: Pseudomonas aeruginosa is the second most prevalent opportunistic pathogen causing nosocomial infections in Mexico. This study evaluated antibiotic resistance, production of virulence factors and clonal diversity of P. aeruginosa strains isolated from patients undergoing nosocomial in...
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The Journal of Infection in Developing Countries
2019-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10953 |
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| author | Eliab M González-Olvera Rebeca Pérez-Morales Alberto González Zamora Graciela Castro-Escarpulli Ingrid Palma-Martínez José J Alba-Romero |
| author_facet | Eliab M González-Olvera Rebeca Pérez-Morales Alberto González Zamora Graciela Castro-Escarpulli Ingrid Palma-Martínez José J Alba-Romero |
| author_sort | Eliab M González-Olvera |
| collection | DOAJ |
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Introduction: Pseudomonas aeruginosa is the second most prevalent opportunistic pathogen causing nosocomial infections in Mexico. This study evaluated antibiotic resistance, production of virulence factors and clonal diversity of P. aeruginosa strains isolated from patients undergoing nosocomial infections in public hospitals of northeastern Mexico.
Methodology: Ninety-two P. aeruginosa isolates from urine culture, Foley catheter, ear, wounds, respiratory tract secretions, scalp, blood culture, bronchoalveolar lavage, expectoration and cerebrospinal fluid causing nosocomial infections were analyzed. The isolates were identified by MALDI-TOF and antibiotic resistance profiles obtained by MicroScan®. The production of virulence factors was analyzed with spectrophotometric techniques and isolates genotyped by ERIC-PCR.
Results: Out of the 92 isolates, 26 (28.2%) were found to be multidrug resistant (MDR); 21 (22.7%) were classified as extremely drug resistant (XDR). Highest resistance rate was found for gatifloxacin (42%) while ciprofloxacin accounted for the antibiotic with the lowest resistance rate (2%). Bronchoalveolar lavage isolates produced the highest amounts of virulence factors: biofilm (44.4% ± 2.7%), elastase (58.5% ± 4.3%), alkaline protease (60.1% ± 5.0%); except for pyocyanin production. The ERIC-PCR assay showed 83 genetic patterns (90% clonal diversity) and 13 isolates had 100% genetic similarity, forming 4 real clones, 3 of these clones were obtained from different anatomical site and/or hospital.
Conclusions: Antibiotic resistance and virulence factors production was heterogeneous among samples analyzed. Genotyping of P. aeruginosa strains showed high genetic diversity in the studied isolates.
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| format | Article |
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| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-617889a7c5bb4bbeab2e16810bd4ef9c2025-08-20T02:14:07ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-05-01130510.3855/jidc.10953Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern MexicoEliab M González-Olvera0Rebeca Pérez-Morales1Alberto González Zamora2Graciela Castro-Escarpulli3Ingrid Palma-Martínez4José J Alba-Romero5Laboratorio de Microbiología Clínica, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Gómez Palacio, Durango, MéxicoLaboratorio de Biología Celular y Molecular, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Gómez Palacio, Durango, MéxicoLaboratorio de Biología Evolutiva, Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Gómez Palacio, Durango, MéxicoLaboratorio de Investigación Clínica y Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MéxicoLaboratorio de Investigación Clínica y Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MéxicoLaboratorio de Microbiología Clínica, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Gómez Palacio, Durango, México Introduction: Pseudomonas aeruginosa is the second most prevalent opportunistic pathogen causing nosocomial infections in Mexico. This study evaluated antibiotic resistance, production of virulence factors and clonal diversity of P. aeruginosa strains isolated from patients undergoing nosocomial infections in public hospitals of northeastern Mexico. Methodology: Ninety-two P. aeruginosa isolates from urine culture, Foley catheter, ear, wounds, respiratory tract secretions, scalp, blood culture, bronchoalveolar lavage, expectoration and cerebrospinal fluid causing nosocomial infections were analyzed. The isolates were identified by MALDI-TOF and antibiotic resistance profiles obtained by MicroScan®. The production of virulence factors was analyzed with spectrophotometric techniques and isolates genotyped by ERIC-PCR. Results: Out of the 92 isolates, 26 (28.2%) were found to be multidrug resistant (MDR); 21 (22.7%) were classified as extremely drug resistant (XDR). Highest resistance rate was found for gatifloxacin (42%) while ciprofloxacin accounted for the antibiotic with the lowest resistance rate (2%). Bronchoalveolar lavage isolates produced the highest amounts of virulence factors: biofilm (44.4% ± 2.7%), elastase (58.5% ± 4.3%), alkaline protease (60.1% ± 5.0%); except for pyocyanin production. The ERIC-PCR assay showed 83 genetic patterns (90% clonal diversity) and 13 isolates had 100% genetic similarity, forming 4 real clones, 3 of these clones were obtained from different anatomical site and/or hospital. Conclusions: Antibiotic resistance and virulence factors production was heterogeneous among samples analyzed. Genotyping of P. aeruginosa strains showed high genetic diversity in the studied isolates. https://jidc.org/index.php/journal/article/view/10953Pseudomonas aeruginosaantibiotic resistanceERIC-PCRvirulence factors |
| spellingShingle | Eliab M González-Olvera Rebeca Pérez-Morales Alberto González Zamora Graciela Castro-Escarpulli Ingrid Palma-Martínez José J Alba-Romero Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico Journal of Infection in Developing Countries Pseudomonas aeruginosa antibiotic resistance ERIC-PCR virulence factors |
| title | Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico |
| title_full | Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico |
| title_fullStr | Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico |
| title_full_unstemmed | Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico |
| title_short | Antibiotic resistance, virulence factors and genotyping of Pseudomonas aeruginosa in public hospitals of northeastern Mexico |
| title_sort | antibiotic resistance virulence factors and genotyping of pseudomonas aeruginosa in public hospitals of northeastern mexico |
| topic | Pseudomonas aeruginosa antibiotic resistance ERIC-PCR virulence factors |
| url | https://jidc.org/index.php/journal/article/view/10953 |
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