Characterization and quantification of proteins secreted by single human embryos prior to implantation
Abstract The use of in vitro fertilization (IVF) has revolutionized the treatment of infertility and is now responsible for 1–5% of all births in industrialized countries. During IVF, it is typical for patients to generate multiple embryos. However, only a small proportion of them possess the geneti...
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| Format: | Article |
| Language: | English |
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Springer Nature
2015-10-01
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| Series: | EMBO Molecular Medicine |
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| Online Access: | https://doi.org/10.15252/emmm.201505344 |
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| author | Maurizio Poli Alessandro Ori Tim Child Souraya Jaroudi Katharina Spath Martin Beck Dagan Wells |
| author_facet | Maurizio Poli Alessandro Ori Tim Child Souraya Jaroudi Katharina Spath Martin Beck Dagan Wells |
| author_sort | Maurizio Poli |
| collection | DOAJ |
| description | Abstract The use of in vitro fertilization (IVF) has revolutionized the treatment of infertility and is now responsible for 1–5% of all births in industrialized countries. During IVF, it is typical for patients to generate multiple embryos. However, only a small proportion of them possess the genetic and metabolic requirements needed in order to produce a healthy pregnancy. The identification of the embryo with the greatest developmental capacity represents a major challenge for fertility clinics. Current methods for the assessment of embryo competence are proven inefficient, and the inadvertent transfer of non‐viable embryos is the principal reason why most IVF treatments (approximately two‐thirds) end in failure. In this study, we investigate how the application of proteomic measurements could improve success rates in clinical embryology. We describe a procedure that allows the identification and quantification of proteins of embryonic origin, present in attomole concentrations in the blastocoel, the enclosed fluid‐filled cavity that forms within 5‐day‐old human embryos. By using targeted proteomics, we demonstrate the feasibility of quantifying multiple proteins in samples derived from single blastocoels and that such measurements correlate with aspects of embryo viability, such as chromosomal (ploidy) status. This study illustrates the potential of high‐sensitivity proteomics to measure clinically relevant biomarkers in minute samples and, more specifically, suggests that key aspects of embryo competence could be measured using a proteomic‐based strategy, with negligible risk of harm to the living embryo. Our work paves the way for the development of “next‐generation” embryo competence assessment strategies, based on functional proteomics. |
| format | Article |
| id | doaj-art-616dbd362a21406a8d0287edcc5d22ba |
| institution | DOAJ |
| issn | 1757-4676 1757-4684 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | EMBO Molecular Medicine |
| spelling | doaj-art-616dbd362a21406a8d0287edcc5d22ba2025-08-20T03:05:55ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842015-10-017111465147910.15252/emmm.201505344Characterization and quantification of proteins secreted by single human embryos prior to implantationMaurizio Poli0Alessandro Ori1Tim Child2Souraya Jaroudi3Katharina Spath4Martin Beck5Dagan Wells6Nuffield Department of Obstetrics and Gynaecology, Institute of Reproductive Sciences, University of OxfordEuropean Molecular Biology Laboratory, Structural and Computational Biology UnitNuffield Department of Obstetrics and Gynaecology, Institute of Reproductive Sciences, University of OxfordReprogenetics UK, Institute of Reproductive SciencesNuffield Department of Obstetrics and Gynaecology, Institute of Reproductive Sciences, University of OxfordEuropean Molecular Biology Laboratory, Structural and Computational Biology UnitNuffield Department of Obstetrics and Gynaecology, Institute of Reproductive Sciences, University of OxfordAbstract The use of in vitro fertilization (IVF) has revolutionized the treatment of infertility and is now responsible for 1–5% of all births in industrialized countries. During IVF, it is typical for patients to generate multiple embryos. However, only a small proportion of them possess the genetic and metabolic requirements needed in order to produce a healthy pregnancy. The identification of the embryo with the greatest developmental capacity represents a major challenge for fertility clinics. Current methods for the assessment of embryo competence are proven inefficient, and the inadvertent transfer of non‐viable embryos is the principal reason why most IVF treatments (approximately two‐thirds) end in failure. In this study, we investigate how the application of proteomic measurements could improve success rates in clinical embryology. We describe a procedure that allows the identification and quantification of proteins of embryonic origin, present in attomole concentrations in the blastocoel, the enclosed fluid‐filled cavity that forms within 5‐day‐old human embryos. By using targeted proteomics, we demonstrate the feasibility of quantifying multiple proteins in samples derived from single blastocoels and that such measurements correlate with aspects of embryo viability, such as chromosomal (ploidy) status. This study illustrates the potential of high‐sensitivity proteomics to measure clinically relevant biomarkers in minute samples and, more specifically, suggests that key aspects of embryo competence could be measured using a proteomic‐based strategy, with negligible risk of harm to the living embryo. Our work paves the way for the development of “next‐generation” embryo competence assessment strategies, based on functional proteomics.https://doi.org/10.15252/emmm.201505344blastocoelgene expressionhuman embryoin vitro fertilizationproteomics |
| spellingShingle | Maurizio Poli Alessandro Ori Tim Child Souraya Jaroudi Katharina Spath Martin Beck Dagan Wells Characterization and quantification of proteins secreted by single human embryos prior to implantation EMBO Molecular Medicine blastocoel gene expression human embryo in vitro fertilization proteomics |
| title | Characterization and quantification of proteins secreted by single human embryos prior to implantation |
| title_full | Characterization and quantification of proteins secreted by single human embryos prior to implantation |
| title_fullStr | Characterization and quantification of proteins secreted by single human embryos prior to implantation |
| title_full_unstemmed | Characterization and quantification of proteins secreted by single human embryos prior to implantation |
| title_short | Characterization and quantification of proteins secreted by single human embryos prior to implantation |
| title_sort | characterization and quantification of proteins secreted by single human embryos prior to implantation |
| topic | blastocoel gene expression human embryo in vitro fertilization proteomics |
| url | https://doi.org/10.15252/emmm.201505344 |
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