Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus

Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus. Selecting an appropriate reference gene is crucial for researching its gene function. In this study, we selected 11 pot...

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Bibliographic Details
Main Authors: Lulu Li, Xiaofan Guo, Shouming Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Microbiology
Subjects:
Inonotus obliquus
reference gene
RT-qPCR
gene expression
medicinal fungus
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1500043/full
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