The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel

This study investigates the influence of different light sources (sunlight, green, red, and white LED) on the germination of <i>Raphanus sativus</i> L. sprouts and the potential use of their sprout extracts in the development of natural dermatocosmetic gels. The bioactive fractions were...

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Main Authors: Mihaela Carmen Eremia, Ramona Daniela Pavaloiu, Oana Livadariu, Anca Daniela Raiciu, Fawzia Sha’at, Corina Bubueanu, Dana Maria Miu
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Gels
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Online Access:https://www.mdpi.com/2310-2861/11/7/515
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author Mihaela Carmen Eremia
Ramona Daniela Pavaloiu
Oana Livadariu
Anca Daniela Raiciu
Fawzia Sha’at
Corina Bubueanu
Dana Maria Miu
author_facet Mihaela Carmen Eremia
Ramona Daniela Pavaloiu
Oana Livadariu
Anca Daniela Raiciu
Fawzia Sha’at
Corina Bubueanu
Dana Maria Miu
author_sort Mihaela Carmen Eremia
collection DOAJ
description This study investigates the influence of different light sources (sunlight, green, red, and white LED) on the germination of <i>Raphanus sativus</i> L. sprouts and the potential use of their sprout extracts in the development of natural dermatocosmetic gels. The bioactive fractions were extracted using simple methods and analyzed for total polyphenol content and antioxidant activity. Statistical analysis of weight, total phenolic content, and antioxidant activity of <i>Raphanus sativus</i> L. sprouts was performed using ANOVA. Sprouts exposed to green LED light showed the highest biomass (16.13 ± 0.38 g), while red LED light resulted in the highest total polyphenol content (3.28 ± 0.03 mg GAE/g fresh weight). The highest antioxidant activity (6.60 ± 0.08 mM Trolox/g fresh weight) was obtained under white LED. Although variations were observed, ANOVA analysis revealed that only sprout weight differed significantly among treatments (<i>p</i> < 0.001), while differences in polyphenol content and antioxidant activity were not statistically significant (<i>p</i> > 0.05). The extract with the highest antioxidant activity was incorporated as an active ingredient into Carbopol-based hydrogel formulations containing natural gelling agents and gentle preservatives. The resulting gels demonstrated favorable pH (4.85–5.05), texture, and stability. The results indicate that the light spectrum influences the germination process and the initial development of seedlings. Moreover, radish sprout extracts, rich in bioactive compounds, show promise for dermatocosmetic applications due to their antioxidant, soothing, and antimicrobial properties. This study supports the use of natural resources in the development of care products, in line with current trends in green cosmetics.
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spelling doaj-art-6158329096db4345aeb35b45c7280fbb2025-08-20T02:45:38ZengMDPI AGGels2310-28612025-07-0111751510.3390/gels11070515The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic GelMihaela Carmen Eremia0Ramona Daniela Pavaloiu1Oana Livadariu2Anca Daniela Raiciu3Fawzia Sha’at4Corina Bubueanu5Dana Maria Miu6National Institute for Chemical-Pharmaceutical Research & Development—ICCF, 112 Vitan Avenue, 3rd District, 031299 Bucharest, RomaniaNational Institute for Chemical-Pharmaceutical Research & Development—ICCF, 112 Vitan Avenue, 3rd District, 031299 Bucharest, RomaniaFaculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Bd. Marasti, 011464 Bucharest, RomaniaFaculty of Pharmacy, Department of Pharmacognosy, University of Titu Maiorescu, Phytochemistry, Phytotherapy, 16 Gh. Sincai Street, 040441 Bucharest, RomaniaNational Institute for Chemical-Pharmaceutical Research & Development—ICCF, 112 Vitan Avenue, 3rd District, 031299 Bucharest, RomaniaNational Institute for Chemical-Pharmaceutical Research & Development—ICCF, 112 Vitan Avenue, 3rd District, 031299 Bucharest, RomaniaNational Institute for Chemical-Pharmaceutical Research & Development—ICCF, 112 Vitan Avenue, 3rd District, 031299 Bucharest, RomaniaThis study investigates the influence of different light sources (sunlight, green, red, and white LED) on the germination of <i>Raphanus sativus</i> L. sprouts and the potential use of their sprout extracts in the development of natural dermatocosmetic gels. The bioactive fractions were extracted using simple methods and analyzed for total polyphenol content and antioxidant activity. Statistical analysis of weight, total phenolic content, and antioxidant activity of <i>Raphanus sativus</i> L. sprouts was performed using ANOVA. Sprouts exposed to green LED light showed the highest biomass (16.13 ± 0.38 g), while red LED light resulted in the highest total polyphenol content (3.28 ± 0.03 mg GAE/g fresh weight). The highest antioxidant activity (6.60 ± 0.08 mM Trolox/g fresh weight) was obtained under white LED. Although variations were observed, ANOVA analysis revealed that only sprout weight differed significantly among treatments (<i>p</i> < 0.001), while differences in polyphenol content and antioxidant activity were not statistically significant (<i>p</i> > 0.05). The extract with the highest antioxidant activity was incorporated as an active ingredient into Carbopol-based hydrogel formulations containing natural gelling agents and gentle preservatives. The resulting gels demonstrated favorable pH (4.85–5.05), texture, and stability. The results indicate that the light spectrum influences the germination process and the initial development of seedlings. Moreover, radish sprout extracts, rich in bioactive compounds, show promise for dermatocosmetic applications due to their antioxidant, soothing, and antimicrobial properties. This study supports the use of natural resources in the development of care products, in line with current trends in green cosmetics.https://www.mdpi.com/2310-2861/11/7/515<i>Raphanus sativus</i> L. seedsgerminationsprout extractspolyphenolsgels
spellingShingle Mihaela Carmen Eremia
Ramona Daniela Pavaloiu
Oana Livadariu
Anca Daniela Raiciu
Fawzia Sha’at
Corina Bubueanu
Dana Maria Miu
The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel
Gels
<i>Raphanus sativus</i> L. seeds
germination
sprout extracts
polyphenols
gels
title The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel
title_full The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel
title_fullStr The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel
title_full_unstemmed The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel
title_short The Effect of Light on the Germination of <i>Raphanus sativus</i> Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel
title_sort effect of light on the germination of i raphanus sativus i seeds and the use of sprout extracts in the development of a dermatocosmetic gel
topic <i>Raphanus sativus</i> L. seeds
germination
sprout extracts
polyphenols
gels
url https://www.mdpi.com/2310-2861/11/7/515
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