Identification of Two <i>R2R3-MYB</i> Genes Involved in Flavan-3-Ols Biosynthesis as Modulated by Salicylic Acid Through RNA-Seq in Grape Berries (<i>Vitis</i> spp.)

Identification of Two <i>R2R3-MYB</i> Genes Involved in Flavan-3-Ols Biosynthesis as Modulated by Salicylic Acid Through RNA-Seq in Grape Berries (<i>Vitis</i> spp.)

Flavan-3-ols are plant secondary metabolites that play important roles in stress resistance. Our previous studies revealed that salicylic acid (SA) activates <i>R2R3-MYB</i> transcription factors, promoting flavan-3-ol biosynthesis. This study identified two <i>R2R3-MYB</i> g...

Full description

Saved in:
Bibliographic Details
Main Authors: Fengchan Yu, Pengfei Zhai, Wenjing Ge, Runtian Zhou, Shuwen Zan, Jinjun Liang, Pengfei Wen, Yu Gary Gao
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Agriculture
Subjects:
<i>Vitis vinifera</i>
salicylic acid
flavan-3-ols
RNA-seq
WGCNA
Online Access:https://www.mdpi.com/2077-0472/14/11/2069
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Flavan-3-ols are plant secondary metabolites that play important roles in stress resistance. Our previous studies revealed that salicylic acid (SA) activates <i>R2R3-MYB</i> transcription factors, promoting flavan-3-ol biosynthesis. This study identified two <i>R2R3-MYB</i> genes that exhibited positive responses to both exogenous SA and were probably involved in flavan-3-ol biosynthesis through RNA-sequencing, functional enrichment analysis, and qRT-PCR. The results indicated that the contents of total flavan-3-ols and their monomers, (+)-catechin and (−)-epicatechin, in grape berries after exogenous SA application were substantially increased compared to those in the control. A total of 683 differentially expressed genes in response to exogenous SA treatment were identified using RNA-seq. KEGG analysis revealed enrichment of the ‘flavonoid biosynthesis’ and ‘plant hormone signal transduction’ pathways. A specific module highly associated with flavan-3-ol biosynthesis was identified by constructing a co-expression network. Two candidate genes (<i>VvMYB108B</i> and <i>VvMYB145</i>) likely participating in flavan-3-ol biosynthesis were selected using qRT-PCR. Therefore, these two potential genes that respond to SA and putatively participate in flavan-3-ol biosynthesis were identified for the first time. These results lay a solid basis for a more profound understanding of the molecular regulation of flavan-3-ol biosynthesis in grapes.
ISSN:2077-0472

Similar Items