Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu)
Some genes like 18S rRNA, 26S rRNA, elongation factor 1-alpha (EF1a), and beta-tubulin (TUB) are members of housekeeping genes group that are commonly used as internal control in gene expression study. This study aimed to isolate those four housekeeping genes of tuntun angin (Elaeocarpus floribundus...
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| Format: | Article |
| Language: | English |
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Universitas Negeri Semarang
2019-08-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/19052 |
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| author | Dewi Indriyani Roslim Ashfira Ashfira Dilla Mutiarawati Tio Fanny Rosmeilinda Nur Aisyah Herman Herman Wahyu Lestari |
| author_facet | Dewi Indriyani Roslim Ashfira Ashfira Dilla Mutiarawati Tio Fanny Rosmeilinda Nur Aisyah Herman Herman Wahyu Lestari |
| author_sort | Dewi Indriyani Roslim |
| collection | DOAJ |
| description | Some genes like 18S rRNA, 26S rRNA, elongation factor 1-alpha (EF1a), and beta-tubulin (TUB) are members of housekeeping genes group that are commonly used as internal control in gene expression study. This study aimed to isolate those four housekeeping genes of tuntun angin (Elaeocarpus floribundus). The research material included fresh leaves of E. floribundus that were picked up from Kajuik Lake in Riau Province and four primer pairs. The procedures consisted of total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), polymerase chain reaction (PCR), electrophoresis on 1% agarose gel, sequencing, and bioinformatic analysis. This study has been isolated 18S rRNA, 26S rRNA, EF1a, and TUB genes with the size of 422 bp, 922 bp, 856 bp, and 877 bp, respectively. The EF1a and TUB genes has never been reported in Elaeocarpaceae family. Thus, those partial DNA sequences are the first sequences reported from this species and can be used as reference genes in this plant after validation. |
| format | Article |
| id | doaj-art-614e25f67a1e4de3adba08a495bc9e9b |
| institution | Kabale University |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-614e25f67a1e4de3adba08a495bc9e9b2025-08-20T03:26:30ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102019-08-0111219420110.15294/biosaintifika.v11i2.190528894Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu)Dewi Indriyani Roslim0Ashfira Ashfira1Dilla Mutiarawati2Tio Fanny Rosmeilinda3Nur Aisyah4Herman Herman5Wahyu Lestari6Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas RiauDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas RiauDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas RiauDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas RiauDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas RiauDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas RiauDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas RiauSome genes like 18S rRNA, 26S rRNA, elongation factor 1-alpha (EF1a), and beta-tubulin (TUB) are members of housekeeping genes group that are commonly used as internal control in gene expression study. This study aimed to isolate those four housekeeping genes of tuntun angin (Elaeocarpus floribundus). The research material included fresh leaves of E. floribundus that were picked up from Kajuik Lake in Riau Province and four primer pairs. The procedures consisted of total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), polymerase chain reaction (PCR), electrophoresis on 1% agarose gel, sequencing, and bioinformatic analysis. This study has been isolated 18S rRNA, 26S rRNA, EF1a, and TUB genes with the size of 422 bp, 922 bp, 856 bp, and 877 bp, respectively. The EF1a and TUB genes has never been reported in Elaeocarpaceae family. Thus, those partial DNA sequences are the first sequences reported from this species and can be used as reference genes in this plant after validation.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/1905218s rrna, 26s rrna, beta-tubulin, ef1, elaeocarpus floribundus |
| spellingShingle | Dewi Indriyani Roslim Ashfira Ashfira Dilla Mutiarawati Tio Fanny Rosmeilinda Nur Aisyah Herman Herman Wahyu Lestari Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) Biosaintifika: Journal of Biology & Biology Education 18s rrna, 26s rrna, beta-tubulin, ef1, elaeocarpus floribundus |
| title | Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) |
| title_full | Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) |
| title_fullStr | Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) |
| title_full_unstemmed | Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) |
| title_short | Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) |
| title_sort | isolation of partial housekeeping genes on tuntun angin elaeocarpus floribundu |
| topic | 18s rrna, 26s rrna, beta-tubulin, ef1, elaeocarpus floribundus |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/19052 |
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