Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1α/VEGF Pathway
Hong-Jie Yan,1,* Fang-Ming Lin,2,* Jing-Jing Li,1 Hao Qin,1 Yi-He Wang,1 Chuan-Hua You1 1Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, 570102, People’s Republic of China; 2Department of Vascular Surgery, Affili...
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Dove Medical Press
2025-06-01
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| Series: | Clinical, Cosmetic and Investigational Dermatology |
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| author | Yan HJ Lin FM Li JJ Qin H Wang YH You CH |
| author_facet | Yan HJ Lin FM Li JJ Qin H Wang YH You CH |
| author_sort | Yan HJ |
| collection | DOAJ |
| description | Hong-Jie Yan,1,&ast; Fang-Ming Lin,2,&ast; Jing-Jing Li,1 Hao Qin,1 Yi-He Wang,1 Chuan-Hua You1 1Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, 570102, People’s Republic of China; 2Department of Vascular Surgery, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou, 570100, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Chuan-Hua You, Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Hainan Medical University, No. 31 of Longhua Street, Longhua District, Haikou, 570102, People’s Republic of China, Tel +8615120793168, Fax +86-1083161294, Email youchuanhua3063@163.comObjective: This study aimed to investigate the effects of botulinum toxin type A (BoTA) on the neovascularisation of diabetic flaps through the factor-1alpha (HIF-1α)/vascular endothelial growth factor (VEGF) pathway.Methods: A total of 60 male Wistar rats (250– 300 g) were randomly divided into 4 groups. Group A consisted of normal rats receiving saline, Group B received BoTA, Group C were diabetic rats treated with saline, and Group D were diabetic rats treated with BoTA. Random-pattern dorsal skin flaps (3× 9 cm) were created, and saline or BoTA was injected at proximal, mid and distal regions. Ten days later, orthotopic flap transplantation was performed. After 7 days, flap survival rate, haematoxylin–eosin (H&E) staining, and the mRNA expression of HIF-1α and VEGF were evaluated.Results: Flap survival area significantly increased in Group B compared to Group A (P < 0.05), and in Group D compared to Group C (P < 0.05). The highest neovascular density was observed in Group B (P < 0.05), while the lowest was in Group C (P < 0.05). No significant difference was found between Groups A and D. Reverse transcription polymerase chain reaction (RT-PCR) showed that HIF-1α and VEGF expression levels were highest in Group B, followed by Groups A, D, and C (P < 0.05).Conclusion: BoTA promotes flap survival and neovascularisation in diabetic rats by enhancing HIF-1α and VEGF expression. These results suggest a potential therapeutic role of BoTA in improving flap outcomes in diabetic patients.Keywords: botulinum toxin type A, flap, diabetes, factor-1alpha |
| format | Article |
| id | doaj-art-614757cb5d784fa4a2fa9d027ca02a3c |
| institution | Kabale University |
| issn | 1178-7015 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Dove Medical Press |
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| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj-art-614757cb5d784fa4a2fa9d027ca02a3c2025-08-20T03:26:57ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152025-06-01Volume 18Issue 115411549104079Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1&alpha;/VEGF PathwayYan HJ0Lin FM1Li JJ2Qin H3Wang YH4You CH5Department of Plastic and Cosmetic SurgeryDepartment of Vascular SurgeryDepartment of Plastic and Cosmetic SurgeryDepartment of Plastic and Cosmetic SurgeryDepartment of Plastic and Cosmetic SurgeryDepartment of Plastic and Cosmetic SurgeryHong-Jie Yan,1,&ast; Fang-Ming Lin,2,&ast; Jing-Jing Li,1 Hao Qin,1 Yi-He Wang,1 Chuan-Hua You1 1Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, 570102, People’s Republic of China; 2Department of Vascular Surgery, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou, 570100, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Chuan-Hua You, Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Hainan Medical University, No. 31 of Longhua Street, Longhua District, Haikou, 570102, People’s Republic of China, Tel +8615120793168, Fax +86-1083161294, Email youchuanhua3063@163.comObjective: This study aimed to investigate the effects of botulinum toxin type A (BoTA) on the neovascularisation of diabetic flaps through the factor-1alpha (HIF-1α)/vascular endothelial growth factor (VEGF) pathway.Methods: A total of 60 male Wistar rats (250– 300 g) were randomly divided into 4 groups. Group A consisted of normal rats receiving saline, Group B received BoTA, Group C were diabetic rats treated with saline, and Group D were diabetic rats treated with BoTA. Random-pattern dorsal skin flaps (3× 9 cm) were created, and saline or BoTA was injected at proximal, mid and distal regions. Ten days later, orthotopic flap transplantation was performed. After 7 days, flap survival rate, haematoxylin–eosin (H&E) staining, and the mRNA expression of HIF-1α and VEGF were evaluated.Results: Flap survival area significantly increased in Group B compared to Group A (P < 0.05), and in Group D compared to Group C (P < 0.05). The highest neovascular density was observed in Group B (P < 0.05), while the lowest was in Group C (P < 0.05). No significant difference was found between Groups A and D. Reverse transcription polymerase chain reaction (RT-PCR) showed that HIF-1α and VEGF expression levels were highest in Group B, followed by Groups A, D, and C (P < 0.05).Conclusion: BoTA promotes flap survival and neovascularisation in diabetic rats by enhancing HIF-1α and VEGF expression. These results suggest a potential therapeutic role of BoTA in improving flap outcomes in diabetic patients.Keywords: botulinum toxin type A, flap, diabetes, factor-1alphahttps://www.dovepress.com/promotion-of-random-flap-neovascularisation-in-rats-with-diabetes-usin-peer-reviewed-fulltext-article-CCIDbotulinum toxin type Aflapdiabetesfactor-1alpha |
| spellingShingle | Yan HJ Lin FM Li JJ Qin H Wang YH You CH Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1&alpha;/VEGF Pathway Clinical, Cosmetic and Investigational Dermatology botulinum toxin type A flap diabetes factor-1alpha |
| title | Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1&alpha;/VEGF Pathway |
| title_full | Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1&alpha;/VEGF Pathway |
| title_fullStr | Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1&alpha;/VEGF Pathway |
| title_full_unstemmed | Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1&alpha;/VEGF Pathway |
| title_short | Promotion of Random Flap Neovascularisation in Rats with Diabetes Using Botulinum Toxin Type A Through the HIF-1&alpha;/VEGF Pathway |
| title_sort | promotion of random flap neovascularisation in rats with diabetes using botulinum toxin type a through the hif 1 amp alpha vegf pathway |
| topic | botulinum toxin type A flap diabetes factor-1alpha |
| url | https://www.dovepress.com/promotion-of-random-flap-neovascularisation-in-rats-with-diabetes-usin-peer-reviewed-fulltext-article-CCID |
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