Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus
The swimming crab (Portunus trituberculatus) is an aquaculture species with high commercial value. However, there is limited information available on the effects of salinity on the lipid metabolism and gut microbiota of P. trituberculatus. This study investigated the hepatopancreatic index, hepatopa...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342400629X |
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| author | Meimei Liu Tong Li Hao Zhang Zi Wang Xugan Wu Jie He Zhiguo Dong |
| author_facet | Meimei Liu Tong Li Hao Zhang Zi Wang Xugan Wu Jie He Zhiguo Dong |
| author_sort | Meimei Liu |
| collection | DOAJ |
| description | The swimming crab (Portunus trituberculatus) is an aquaculture species with high commercial value. However, there is limited information available on the effects of salinity on the lipid metabolism and gut microbiota of P. trituberculatus. This study investigated the hepatopancreatic index, hepatopancreatic lipid metabolism, physiological and biochemical indicators and gut microbiota of P. trituberculatus cultured at salinity 28 ‰, 25 ‰, 22 ‰ and 19 ‰ for 8 weeks. The results showed that the highest expression level of SREBP1, FAS, LPR1, ACOX and FABP was found in 19 ‰ group compared to the 28 ‰ group, along with the enzyme activities of CPT1 and FAS. On the contrary, the expression level of DGAT1 and LPR2 in the 28 ‰ group was significantly higher than that of 19 ‰ group, along with the enzyme activities of LPS. The results of fatty acid content showed that only polyunsaturated fatty acids showed significant changes in response to salinity, such as C18:2n6, C20:2n6 and C20:3n3. Moreover, the intestine microbial α-diversity index of crab in the 28 ‰ treatment was higher than that of other groups. The dominant phylum in gut samples was Proteobacteria, followed by Firmicutes and Bacteroidota. Moreover, Mycoplasma, Candidatus Endoecteinascidia and Shewanella showed a significant decline in the 28 ‰ group compared to the 19 ‰ group. Bacterial functional pathway prediction has also shown that the KEGG pathways involved in ovarian steroidogenesis, NF-kappa B signaling pathway and regulation of lipolysis in adipocytes increased in 28 ‰ group compared with 19 ‰ group. In conclusion, hepatopancreatic fatty acid metabolism was more active in the 19 ‰ group, whereas the 28 ‰ group not only promoted triglyceride synthesis in the hepatopancreas, but also facilitated ovarian development of P. trituberculatus by influencing the gut microbial composition. |
| format | Article |
| id | doaj-art-614014f160734a1cb9197f58a78e8fc5 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-614014f160734a1cb9197f58a78e8fc52025-02-06T05:12:04ZengElsevierAquaculture Reports2352-51342025-03-0140102541Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatusMeimei Liu0Tong Li1Hao Zhang2Zi Wang3Xugan Wu4Jie He5Zhiguo Dong6Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, ChinaCentre for Research on Environmental Ecology and Fish Nutrition of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, ChinaZhejiang Marine Fisheries Research Institute, Key Laboratory of Mariculture & Enhancement of Zhejiang Province, Zhoushan, China; Corresponding author.Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Institute of Marine Resources Development, Lianyungang 222005, China; Corresponding author at: Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China.The swimming crab (Portunus trituberculatus) is an aquaculture species with high commercial value. However, there is limited information available on the effects of salinity on the lipid metabolism and gut microbiota of P. trituberculatus. This study investigated the hepatopancreatic index, hepatopancreatic lipid metabolism, physiological and biochemical indicators and gut microbiota of P. trituberculatus cultured at salinity 28 ‰, 25 ‰, 22 ‰ and 19 ‰ for 8 weeks. The results showed that the highest expression level of SREBP1, FAS, LPR1, ACOX and FABP was found in 19 ‰ group compared to the 28 ‰ group, along with the enzyme activities of CPT1 and FAS. On the contrary, the expression level of DGAT1 and LPR2 in the 28 ‰ group was significantly higher than that of 19 ‰ group, along with the enzyme activities of LPS. The results of fatty acid content showed that only polyunsaturated fatty acids showed significant changes in response to salinity, such as C18:2n6, C20:2n6 and C20:3n3. Moreover, the intestine microbial α-diversity index of crab in the 28 ‰ treatment was higher than that of other groups. The dominant phylum in gut samples was Proteobacteria, followed by Firmicutes and Bacteroidota. Moreover, Mycoplasma, Candidatus Endoecteinascidia and Shewanella showed a significant decline in the 28 ‰ group compared to the 19 ‰ group. Bacterial functional pathway prediction has also shown that the KEGG pathways involved in ovarian steroidogenesis, NF-kappa B signaling pathway and regulation of lipolysis in adipocytes increased in 28 ‰ group compared with 19 ‰ group. In conclusion, hepatopancreatic fatty acid metabolism was more active in the 19 ‰ group, whereas the 28 ‰ group not only promoted triglyceride synthesis in the hepatopancreas, but also facilitated ovarian development of P. trituberculatus by influencing the gut microbial composition.http://www.sciencedirect.com/science/article/pii/S235251342400629XPortunus trituberculatusSalinityHepatopancreasLipid metabolismIntestinal microbiota |
| spellingShingle | Meimei Liu Tong Li Hao Zhang Zi Wang Xugan Wu Jie He Zhiguo Dong Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus Aquaculture Reports Portunus trituberculatus Salinity Hepatopancreas Lipid metabolism Intestinal microbiota |
| title | Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus |
| title_full | Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus |
| title_fullStr | Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus |
| title_full_unstemmed | Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus |
| title_short | Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus |
| title_sort | salinity affects the lipid metabolism and intestinal microbial composition of swimming crab portunus trituberculatus |
| topic | Portunus trituberculatus Salinity Hepatopancreas Lipid metabolism Intestinal microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S235251342400629X |
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