Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows
Dietary tannin supplementation represents a potential strategy to modulate rumen fermentation and enhance lactation performance in dairy cows, though responses remain inconsistent. A 21-day feeding trial was conducted to evaluate the effect of chestnut tannin (CNT) extract on the enteric methane emi...
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2025-07-01
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| author | Radiša Prodanović Dušan Bošnjaković Ana Djordjevic Predrag Simeunović Sveta Arsić Aleksandra Mitrović Ljubomir Jovanović Ivan Vujanac Danijela Kirovski Sreten Nedić |
| author_facet | Radiša Prodanović Dušan Bošnjaković Ana Djordjevic Predrag Simeunović Sveta Arsić Aleksandra Mitrović Ljubomir Jovanović Ivan Vujanac Danijela Kirovski Sreten Nedić |
| author_sort | Radiša Prodanović |
| collection | DOAJ |
| description | Dietary tannin supplementation represents a potential strategy to modulate rumen fermentation and enhance lactation performance in dairy cows, though responses remain inconsistent. A 21-day feeding trial was conducted to evaluate the effect of chestnut tannin (CNT) extract on the enteric methane emissions (EME), blood metabolites, and milk production traits in mid-lactation dairy cows. Thirty-six Holstein cows were allocated to three homogeneous treatment groups: control (CNT<sub>0</sub>, 0 g/d CNT), CNT<sub>40</sub> (40 g/d CNT), and CNT<sub>80</sub> (80 g/d CNT). Measurements of EME, dry matter intake (DMI), milk yield (MY), and blood and milk parameters were carried out pre- and post-21-day supplementation period. Compared with the no-additive group, the CNT extract reduced methane production, methane yield, and methane intensity in CNT<sub>40</sub> and CNT<sub>80</sub> (<i>p</i> < 0.001). CNT<sub>40</sub> and CNT<sub>80</sub> cows exhibited lower blood urea nitrogen (<i>p</i> = 0.019 and <i>p</i> = 0.002) and elevated serum insulin (<i>p</i> = 0.003 and <i>p</i> < 0.001) and growth hormone concentrations (<i>p</i> = 0.046 and <i>p</i> = 0.034), coinciding with reduced aspartate aminotransferase (<i>p</i> = 0.016 and <i>p</i> = 0.045), and lactate dehydrogenase (<i>p =</i> 0.011 and <i>p =</i> 0.008) activities compared to control. However, CNT<sub>80</sub> had higher circulating NEFA and BHBA than CNT<sub>0</sub> (<i>p</i> = 0.003 and <i>p</i> = 0.004) and CNT<sub>40</sub> (<i>p</i> = 0.035 and <i>p</i> = 0.019). The blood glucose, albumin, and total bilirubin concentrations were not affected. MY and fat- and protein-corrected milk (FPCM), MY/DMI, and FPCM/DMI were higher in both CNT<sub>40</sub> (<i>p</i> = 0.004, <i>p</i> = 0.003, <i>p</i> = 0.014, <i>p</i> = 0.010) and CNT<sub>80</sub> (<i>p</i> = 0.002, <i>p</i> = 0.003, <i>p</i> = 0.008, <i>p</i> = 0.013) cows compared with controls. Feeding CNT<sub>80</sub> resulted in higher protein content (<i>p</i> = 0.015) but lower fat percentage in milk (<i>p</i> = 0.004) compared to CNT<sub>0</sub>. Milk urea nitrogen and somatic cell counts were significantly lower in both CNT<sub>40</sub> (<i>p</i> < 0.001, <i>p</i> = 0.009) and CNT<sub>80</sub> (<i>p</i> < 0.001 for both) compared to CNT<sub>0</sub>, while milk lactose did not differ between treatments. These findings demonstrate that chestnut tannin extract effectively mitigates EME while enhancing lactation performance in mid-lactation dairy cows. |
| format | Article |
| id | doaj-art-613fb17ae906487a80780d84b8339a8a |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-613fb17ae906487a80780d84b8339a8a2025-08-20T03:02:48ZengMDPI AGAnimals2076-26152025-07-011515223810.3390/ani15152238Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation CowsRadiša Prodanović0Dušan Bošnjaković1Ana Djordjevic2Predrag Simeunović3Sveta Arsić4Aleksandra Mitrović5Ljubomir Jovanović6Ivan Vujanac7Danijela Kirovski8Sreten Nedić9Department of Ruminant and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Biochemistry, Institute for Biological Research “Siniša Stankovic”-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, SerbiaSabac Veterinary Station, Vojvode Putnika 52, 15000 Sabac, SerbiaDepartment of Ruminant and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Ruminant and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Ruminant and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Ruminant and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDietary tannin supplementation represents a potential strategy to modulate rumen fermentation and enhance lactation performance in dairy cows, though responses remain inconsistent. A 21-day feeding trial was conducted to evaluate the effect of chestnut tannin (CNT) extract on the enteric methane emissions (EME), blood metabolites, and milk production traits in mid-lactation dairy cows. Thirty-six Holstein cows were allocated to three homogeneous treatment groups: control (CNT<sub>0</sub>, 0 g/d CNT), CNT<sub>40</sub> (40 g/d CNT), and CNT<sub>80</sub> (80 g/d CNT). Measurements of EME, dry matter intake (DMI), milk yield (MY), and blood and milk parameters were carried out pre- and post-21-day supplementation period. Compared with the no-additive group, the CNT extract reduced methane production, methane yield, and methane intensity in CNT<sub>40</sub> and CNT<sub>80</sub> (<i>p</i> < 0.001). CNT<sub>40</sub> and CNT<sub>80</sub> cows exhibited lower blood urea nitrogen (<i>p</i> = 0.019 and <i>p</i> = 0.002) and elevated serum insulin (<i>p</i> = 0.003 and <i>p</i> < 0.001) and growth hormone concentrations (<i>p</i> = 0.046 and <i>p</i> = 0.034), coinciding with reduced aspartate aminotransferase (<i>p</i> = 0.016 and <i>p</i> = 0.045), and lactate dehydrogenase (<i>p =</i> 0.011 and <i>p =</i> 0.008) activities compared to control. However, CNT<sub>80</sub> had higher circulating NEFA and BHBA than CNT<sub>0</sub> (<i>p</i> = 0.003 and <i>p</i> = 0.004) and CNT<sub>40</sub> (<i>p</i> = 0.035 and <i>p</i> = 0.019). The blood glucose, albumin, and total bilirubin concentrations were not affected. MY and fat- and protein-corrected milk (FPCM), MY/DMI, and FPCM/DMI were higher in both CNT<sub>40</sub> (<i>p</i> = 0.004, <i>p</i> = 0.003, <i>p</i> = 0.014, <i>p</i> = 0.010) and CNT<sub>80</sub> (<i>p</i> = 0.002, <i>p</i> = 0.003, <i>p</i> = 0.008, <i>p</i> = 0.013) cows compared with controls. Feeding CNT<sub>80</sub> resulted in higher protein content (<i>p</i> = 0.015) but lower fat percentage in milk (<i>p</i> = 0.004) compared to CNT<sub>0</sub>. Milk urea nitrogen and somatic cell counts were significantly lower in both CNT<sub>40</sub> (<i>p</i> < 0.001, <i>p</i> = 0.009) and CNT<sub>80</sub> (<i>p</i> < 0.001 for both) compared to CNT<sub>0</sub>, while milk lactose did not differ between treatments. These findings demonstrate that chestnut tannin extract effectively mitigates EME while enhancing lactation performance in mid-lactation dairy cows.https://www.mdpi.com/2076-2615/15/15/2238supplementsmethane mitigationhormonal statusmetabolic statusmilk yieldsustainability |
| spellingShingle | Radiša Prodanović Dušan Bošnjaković Ana Djordjevic Predrag Simeunović Sveta Arsić Aleksandra Mitrović Ljubomir Jovanović Ivan Vujanac Danijela Kirovski Sreten Nedić Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows Animals supplements methane mitigation hormonal status metabolic status milk yield sustainability |
| title | Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows |
| title_full | Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows |
| title_fullStr | Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows |
| title_full_unstemmed | Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows |
| title_short | Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows |
| title_sort | effects of chestnut tannin extract on enteric methane emissions blood metabolites and lactation performance in mid lactation cows |
| topic | supplements methane mitigation hormonal status metabolic status milk yield sustainability |
| url | https://www.mdpi.com/2076-2615/15/15/2238 |
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