Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit
The aim of this work was to assess and compare different extraction methods by using high hydrostatic pressure (HHPE), ultrasound (UE), agitation (AE), and their combinations for the extraction of bioactive compounds of Chilean papaya. Extract antioxidant capacity was evaluated by three methods (i.e...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/347532 |
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| author | Elsa Uribe Alvaro Delgadillo Claudia Giovagnoli-Vicuña Issis Quispe-Fuentes Liliana Zura-Bravo |
| author_facet | Elsa Uribe Alvaro Delgadillo Claudia Giovagnoli-Vicuña Issis Quispe-Fuentes Liliana Zura-Bravo |
| author_sort | Elsa Uribe |
| collection | DOAJ |
| description | The aim of this work was to assess and compare different extraction methods by using high hydrostatic pressure (HHPE), ultrasound (UE), agitation (AE), and their combinations for the extraction of bioactive compounds of Chilean papaya. Extract antioxidant capacity was evaluated by three methods (i.e., DPPH, FRAP, and Voltammetry) and phenolic compounds and vitamin C were determined by HPLC. Papaya sample extraction was performed by HHPE at 500 MPa for 10 min and UE and AE for 30 min, respectively. The combined-extractions: HHPE-UE and HHPE-AE, were carried out for 5 min and 15 min, respectively. The highest values found were total phenolic 129.1 mg GAE/100 g FW, antioxidant capacity by DPPH 20.6 mM TE/100 g FW, and voltammetry 141.0 mM TE/100 g FW for HHPE-UE method in free compound extraction. Regarding vitamin C content, its highest value was found by HHPE-UE (74 mg/100 g FW) a combined extraction method. The phenolic compounds rutin and p-coumaric acid were found in all the extracts, both in free and bound forms, respectively. Besides, the combined techniques improved the extraction of bioactive compounds. |
| format | Article |
| id | doaj-art-61340b9078bb4c7aa7963e5b8708ef21 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-61340b9078bb4c7aa7963e5b8708ef212025-08-20T03:34:57ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/347532347532Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) FruitElsa Uribe0Alvaro Delgadillo1Claudia Giovagnoli-Vicuña2Issis Quispe-Fuentes3Liliana Zura-Bravo4Departamento de Ingeniería en Alimentos, Universidad de La Serena, Avenida Raúl Bitrán No. 1305, Casilla 599, 1720010 La Serena, ChileDepartamento de Química, Universidad de La Serena, Avenida Raúl Bitrán No. 1305, Casilla 599, 1720010 La Serena, ChileDepartamento de Ingeniería en Alimentos, Universidad de La Serena, Avenida Raúl Bitrán No. 1305, Casilla 599, 1720010 La Serena, ChileDepartamento de Ingeniería en Alimentos, Universidad de La Serena, Avenida Raúl Bitrán No. 1305, Casilla 599, 1720010 La Serena, ChileDepartamento de Ingeniería en Alimentos, Universidad de La Serena, Avenida Raúl Bitrán No. 1305, Casilla 599, 1720010 La Serena, ChileThe aim of this work was to assess and compare different extraction methods by using high hydrostatic pressure (HHPE), ultrasound (UE), agitation (AE), and their combinations for the extraction of bioactive compounds of Chilean papaya. Extract antioxidant capacity was evaluated by three methods (i.e., DPPH, FRAP, and Voltammetry) and phenolic compounds and vitamin C were determined by HPLC. Papaya sample extraction was performed by HHPE at 500 MPa for 10 min and UE and AE for 30 min, respectively. The combined-extractions: HHPE-UE and HHPE-AE, were carried out for 5 min and 15 min, respectively. The highest values found were total phenolic 129.1 mg GAE/100 g FW, antioxidant capacity by DPPH 20.6 mM TE/100 g FW, and voltammetry 141.0 mM TE/100 g FW for HHPE-UE method in free compound extraction. Regarding vitamin C content, its highest value was found by HHPE-UE (74 mg/100 g FW) a combined extraction method. The phenolic compounds rutin and p-coumaric acid were found in all the extracts, both in free and bound forms, respectively. Besides, the combined techniques improved the extraction of bioactive compounds.http://dx.doi.org/10.1155/2015/347532 |
| spellingShingle | Elsa Uribe Alvaro Delgadillo Claudia Giovagnoli-Vicuña Issis Quispe-Fuentes Liliana Zura-Bravo Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit Journal of Chemistry |
| title | Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit |
| title_full | Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit |
| title_fullStr | Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit |
| title_full_unstemmed | Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit |
| title_short | Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit |
| title_sort | extraction techniques for bioactive compounds and antioxidant capacity determination of chilean papaya vasconcellea pubescens fruit |
| url | http://dx.doi.org/10.1155/2015/347532 |
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