Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line
Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothio...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2024/8885068 |
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| author | Ebtisam Yousef A. Alnakeeb Ahmad Faizal Abdull Razis Kim Wei Chan Chau Ling Tham Yee Han Chan Anwar Salm Kalifa Kafo Nuzul Noorahya Jambari Patrick Rollin Florence Djedaini-Pilard |
| author_facet | Ebtisam Yousef A. Alnakeeb Ahmad Faizal Abdull Razis Kim Wei Chan Chau Ling Tham Yee Han Chan Anwar Salm Kalifa Kafo Nuzul Noorahya Jambari Patrick Rollin Florence Djedaini-Pilard |
| author_sort | Ebtisam Yousef A. Alnakeeb |
| collection | DOAJ |
| description | Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha-cyclodextrin-moringin (α-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The α-CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α-CD/MG. The release of beta-hexosaminidase (β-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-α), and interleukin (IL-4) was considered in the late stage. Higher concentrations of α-CD/MG (5 μM, p<0.001) in mast cell degranulation significantly inhibited the expression of β-hexosaminidase, histamine, TNF-α, PGD2, and IL-4 in both the early and late stages. Thus, α-CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages. |
| format | Article |
| id | doaj-art-6133cd325dcf4797b52878511a6dce81 |
| institution | Kabale University |
| issn | 2633-4690 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-6133cd325dcf4797b52878511a6dce812025-02-03T06:06:37ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902024-01-01202410.1155/2024/8885068Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell LineEbtisam Yousef A. Alnakeeb0Ahmad Faizal Abdull Razis1Kim Wei Chan2Chau Ling Tham3Yee Han Chan4Anwar Salm Kalifa Kafo5Nuzul Noorahya Jambari6Patrick Rollin7Florence Djedaini-Pilard8Natural Medicines and Products Research LaboratoryNatural Medicines and Products Research LaboratoryNatural Medicines and Products Research LaboratoryDepartment of Biomedical SciencesDepartment of Biomedical SciencesDepartment of PathologyLaboratory of Food Safety and Food IntegrityUniversité d’Orléans et CNRSLG2A UR 7378Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha-cyclodextrin-moringin (α-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The α-CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α-CD/MG. The release of beta-hexosaminidase (β-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-α), and interleukin (IL-4) was considered in the late stage. Higher concentrations of α-CD/MG (5 μM, p<0.001) in mast cell degranulation significantly inhibited the expression of β-hexosaminidase, histamine, TNF-α, PGD2, and IL-4 in both the early and late stages. Thus, α-CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages.http://dx.doi.org/10.1155/2024/8885068 |
| spellingShingle | Ebtisam Yousef A. Alnakeeb Ahmad Faizal Abdull Razis Kim Wei Chan Chau Ling Tham Yee Han Chan Anwar Salm Kalifa Kafo Nuzul Noorahya Jambari Patrick Rollin Florence Djedaini-Pilard Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line Advances in Pharmacological and Pharmaceutical Sciences |
| title | Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line |
| title_full | Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line |
| title_fullStr | Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line |
| title_full_unstemmed | Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line |
| title_short | Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line |
| title_sort | antiallergic effect of the alpha cyclodextrin moringin complex in rat basophilic leukaemia rbl 2h3 cell line |
| url | http://dx.doi.org/10.1155/2024/8885068 |
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