Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats

Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents w...

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Main Authors: Takashi Kanamoto, Nazariy Souchelnytskyi, Takuji Kurimoto, Yasuhiro Ikeda, Hiroaki Sakaue, Yasunari Munemasa, Yoshiaki Kiuchi
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Journal of Ophthalmology
Online Access:http://dx.doi.org/10.1155/2015/492050
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author Takashi Kanamoto
Nazariy Souchelnytskyi
Takuji Kurimoto
Yasuhiro Ikeda
Hiroaki Sakaue
Yasunari Munemasa
Yoshiaki Kiuchi
author_facet Takashi Kanamoto
Nazariy Souchelnytskyi
Takuji Kurimoto
Yasuhiro Ikeda
Hiroaki Sakaue
Yasunari Munemasa
Yoshiaki Kiuchi
author_sort Takashi Kanamoto
collection DOAJ
description Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents while the right eyes received no treatment and served as controls. After TES, the eye was enucleated and the retina was isolated. The retinas were analyzed by proteomics. Results. Proteomics showed that twenty-five proteins were upregulated by TES. The identified proteins included cellular signaling proteins, proteins associated with neuronal transmission, metabolic proteins, immunological factors, and structural proteins. Conclusions. TES induced changes in expression of various functional proteins in the retina.
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institution Kabale University
issn 2090-004X
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language English
publishDate 2015-01-01
publisher Wiley
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series Journal of Ophthalmology
spelling doaj-art-6117dcd239d846b1b7d6e90cc8d672b82025-08-20T03:34:57ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/492050492050Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in RatsTakashi Kanamoto0Nazariy Souchelnytskyi1Takuji Kurimoto2Yasuhiro Ikeda3Hiroaki Sakaue4Yasunari Munemasa5Yoshiaki Kiuchi6Department of Ophtahlomology, Hiroshima Memorial Hospital, 1-4-3 Honkawacho, Minami-ku, Hiroshima 730-0802, JapanPersonalized Cancer Medicine Unit, Department of Oncology-Pathology, Karolinska Institute, 171 77 Stockholm, SwedenDepartment of Ophthalmology, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-shi, Osaka 569-8686, JapanDepartment of Ophthalmology, Kyushu University, 3-1-1 Maedashi, Higashi-ku, Fukuoka 812-8592, JapanDepartment of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Kitakanemaru, Tahara-shi, Tochigi 324-8501, JapanDepartment of Ophthalmology, St. Marianna Medical College, 2-16-1 Sugao, Miyamae-ku, Kawasaki-shi, Kanagawa 216-8511, JapanDepartment of Ophthalmology and Visual Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshiam 734-8551, JapanBackground. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents while the right eyes received no treatment and served as controls. After TES, the eye was enucleated and the retina was isolated. The retinas were analyzed by proteomics. Results. Proteomics showed that twenty-five proteins were upregulated by TES. The identified proteins included cellular signaling proteins, proteins associated with neuronal transmission, metabolic proteins, immunological factors, and structural proteins. Conclusions. TES induced changes in expression of various functional proteins in the retina.http://dx.doi.org/10.1155/2015/492050
spellingShingle Takashi Kanamoto
Nazariy Souchelnytskyi
Takuji Kurimoto
Yasuhiro Ikeda
Hiroaki Sakaue
Yasunari Munemasa
Yoshiaki Kiuchi
Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
Journal of Ophthalmology
title Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_full Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_fullStr Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_full_unstemmed Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_short Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_sort proteomic study of retinal proteins associated with transcorneal electric stimulation in rats
url http://dx.doi.org/10.1155/2015/492050
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