Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy
Fasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the enti...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/462084 |
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| author | Sultan Ayaz Riaz Ullah Naser M. AbdEl-Salam Sumiara Shams Sadaf Niaz |
| author_facet | Sultan Ayaz Riaz Ullah Naser M. AbdEl-Salam Sumiara Shams Sadaf Niaz |
| author_sort | Sultan Ayaz |
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| description | Fasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the entire study. A total of 307 samples including 149 samples from Karak and 158 samples from Kohat abattoirs were examined by PCR method and overall prevalence of fasciolosis was 5.86% (18/307), amongst theses 8.05% (12/149) in liver biopsy and 3.79% (6/158) in feacal samples of cattle and Buffaloes were recorded. Similarly the microscopy based detection was 3.58% (11/307) including 4.61% (7/149) in liver biopsy and 2.5% (4/158) in faecal samples accordingly. Furthermore the areawise prevalence of fasciolosis in abattoirs by PCR method was found to be 7.59% (12/158) in Kohat and 4.02% (6/149) in Karak. A 618 pb DNA was amplified in 2% agarose gel electrophoreses. It is concluded from the study that prevalence of fasciolosis was higher in abattoir of district Kohat and PCR was a more sensitive method of diagnosis than microscopy. |
| format | Article |
| id | doaj-art-6113b514e3d14b9c96228a454bd63856 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6113b514e3d14b9c96228a454bd638562025-02-03T01:31:25ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/462084462084Fasciola hepatica in Some Buffaloes and Cattle by PCR and MicroscopySultan Ayaz0Riaz Ullah1Naser M. AbdEl-Salam2Sumiara Shams3Sadaf Niaz4College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa 23200, PakistanDepartment of Chemistry, Government College Ara Khel, FR Kohat, Khyber Pakhtunkhwa 26000, PakistanRiyadh Community College, King Saud University, Riyadh 11437, Saudi ArabiaDepartment of Zoology, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa 23200, PakistanDepartment of Zoology, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa 23200, PakistanFasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the entire study. A total of 307 samples including 149 samples from Karak and 158 samples from Kohat abattoirs were examined by PCR method and overall prevalence of fasciolosis was 5.86% (18/307), amongst theses 8.05% (12/149) in liver biopsy and 3.79% (6/158) in feacal samples of cattle and Buffaloes were recorded. Similarly the microscopy based detection was 3.58% (11/307) including 4.61% (7/149) in liver biopsy and 2.5% (4/158) in faecal samples accordingly. Furthermore the areawise prevalence of fasciolosis in abattoirs by PCR method was found to be 7.59% (12/158) in Kohat and 4.02% (6/149) in Karak. A 618 pb DNA was amplified in 2% agarose gel electrophoreses. It is concluded from the study that prevalence of fasciolosis was higher in abattoir of district Kohat and PCR was a more sensitive method of diagnosis than microscopy.http://dx.doi.org/10.1155/2014/462084 |
| spellingShingle | Sultan Ayaz Riaz Ullah Naser M. AbdEl-Salam Sumiara Shams Sadaf Niaz Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy The Scientific World Journal |
| title | Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy |
| title_full | Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy |
| title_fullStr | Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy |
| title_full_unstemmed | Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy |
| title_short | Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy |
| title_sort | fasciola hepatica in some buffaloes and cattle by pcr and microscopy |
| url | http://dx.doi.org/10.1155/2014/462084 |
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