UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids
Polyphenols are natural substances that are consumed in the daily diet and are found abundantly in a diverse range of fruits and vegetables. Deep eutectic solvent is a new kind of green solvent, which is widely used in the extraction and separation of polyphenols because it is non-toxic and pollutio...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2024-01-01
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| Series: | Beverage Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/bpr-0024-0031 |
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| author | Xiaoxian Lu Zi Ye Lijie Jiang Lele Wen Juzhao Liu Qi Cui |
| author_facet | Xiaoxian Lu Zi Ye Lijie Jiang Lele Wen Juzhao Liu Qi Cui |
| author_sort | Xiaoxian Lu |
| collection | DOAJ |
| description | Polyphenols are natural substances that are consumed in the daily diet and are found abundantly in a diverse range of fruits and vegetables. Deep eutectic solvent is a new kind of green solvent, which is widely used in the extraction and separation of polyphenols because it is non-toxic and pollution-free, and meets the requirements of environmental protection. This research used ultrasonic-assisted deep eutectic solvent (glycerol/urea 1:2, mol/mol, 30% water content) to extract polyphenols from pomegranate peel with improved extraction efficiency. The extracts were analyzed and identified by UPLC-Q-TOF-MS/MS, and 53 compounds were obtained, of which 10 were ellagic acid and its derivatives, and 32 were flavonoids. These polyphenols exhibit a wide range of pharmacological properties, including, but not limited to anti-inflammatory and antioxidant effects. Long-term intake of these polyphenols can effectively prevent chronic diseases, such as cardiovascular and cerebrovascular diseases, chronic tumors, etc. Polyphenols generally have good water solubility, especially anthocyanin compounds with cationic structures that have the best water solubility. Therefore, the incorporation of polyphenols into water to prepare tea and its regular consumption confers significant health benefits. Compared with acarbose, Punicalagin had a better inhibitory ability on α-glucosidase with a binding affinity of −12.2 kcal/mol and 10 hydrogen bonds were formed, indicating that Punicalagin had a potential anti-diabetic function. Pomegranate peel, a by-product of the pomegranate fruit, is typically discarded as waste in daily operations. However, exploring secondary applications for pomegranate peel not only contributes to cost reduction for enterprises but also promotes environmental sustainability and facilitates sustainable development. The extraction technology of pomegranate peel polyphenols also provides a new idea and method for the deep processing of agricultural products. This work will help to understand the biosynthetic pathways of pomegranate peel polyphenols, to facilitate the development of beverages containing pomegranate peel polyphenols. |
| format | Article |
| id | doaj-art-611257e577ac4f5fa8f8f0b2e9d14065 |
| institution | DOAJ |
| issn | 2769-2108 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Beverage Plant Research |
| spelling | doaj-art-611257e577ac4f5fa8f8f0b2e9d140652025-08-20T03:18:31ZengMaximum Academic PressBeverage Plant Research2769-21082024-01-014111010.48130/bpr-0024-0031bpr-0024-0031UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoidsXiaoxian Lu0Zi Ye1Lijie Jiang2Lele Wen3Juzhao Liu4Qi Cui5School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, PR ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, PR ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, PR ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, PR ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, PR ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, PR ChinaPolyphenols are natural substances that are consumed in the daily diet and are found abundantly in a diverse range of fruits and vegetables. Deep eutectic solvent is a new kind of green solvent, which is widely used in the extraction and separation of polyphenols because it is non-toxic and pollution-free, and meets the requirements of environmental protection. This research used ultrasonic-assisted deep eutectic solvent (glycerol/urea 1:2, mol/mol, 30% water content) to extract polyphenols from pomegranate peel with improved extraction efficiency. The extracts were analyzed and identified by UPLC-Q-TOF-MS/MS, and 53 compounds were obtained, of which 10 were ellagic acid and its derivatives, and 32 were flavonoids. These polyphenols exhibit a wide range of pharmacological properties, including, but not limited to anti-inflammatory and antioxidant effects. Long-term intake of these polyphenols can effectively prevent chronic diseases, such as cardiovascular and cerebrovascular diseases, chronic tumors, etc. Polyphenols generally have good water solubility, especially anthocyanin compounds with cationic structures that have the best water solubility. Therefore, the incorporation of polyphenols into water to prepare tea and its regular consumption confers significant health benefits. Compared with acarbose, Punicalagin had a better inhibitory ability on α-glucosidase with a binding affinity of −12.2 kcal/mol and 10 hydrogen bonds were formed, indicating that Punicalagin had a potential anti-diabetic function. Pomegranate peel, a by-product of the pomegranate fruit, is typically discarded as waste in daily operations. However, exploring secondary applications for pomegranate peel not only contributes to cost reduction for enterprises but also promotes environmental sustainability and facilitates sustainable development. The extraction technology of pomegranate peel polyphenols also provides a new idea and method for the deep processing of agricultural products. This work will help to understand the biosynthetic pathways of pomegranate peel polyphenols, to facilitate the development of beverages containing pomegranate peel polyphenols.https://www.maxapress.com/article/doi/10.48130/bpr-0024-0031pomegranate peelpolyphenolsdeep eutectic solventsuplc-q-tof-ms/msellagic acidflavonoids |
| spellingShingle | Xiaoxian Lu Zi Ye Lijie Jiang Lele Wen Juzhao Liu Qi Cui UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids Beverage Plant Research pomegranate peel polyphenols deep eutectic solvents uplc-q-tof-ms/ms ellagic acid flavonoids |
| title | UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids |
| title_full | UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids |
| title_fullStr | UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids |
| title_full_unstemmed | UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids |
| title_short | UPLC-Q-TOF-MS/MS analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids |
| title_sort | uplc q tof ms ms analysis of pomegranate peel polyphenols and preliminary study on the biosynthesis of ellagic acid and flavonoids |
| topic | pomegranate peel polyphenols deep eutectic solvents uplc-q-tof-ms/ms ellagic acid flavonoids |
| url | https://www.maxapress.com/article/doi/10.48130/bpr-0024-0031 |
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