The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada
ObjectivesThe purpose of this study was to characterize the prevalence of antimicrobial resistance in Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from healthy feedlot cattle over 2 years, and investigate factors potentially associated with recovery of resistant isol...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1497402/full |
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| author | Porjai Rattanapanadda Porjai Rattanapanadda Dana Ramsay Alyssa Butters Calvin W. Booker Sherry J. Hannon Steve Hendrick Joyce Van Donkersgoed Brian N. Warr Sheryl P. Gow Paul S. Morley |
| author_facet | Porjai Rattanapanadda Porjai Rattanapanadda Dana Ramsay Alyssa Butters Calvin W. Booker Sherry J. Hannon Steve Hendrick Joyce Van Donkersgoed Brian N. Warr Sheryl P. Gow Paul S. Morley |
| author_sort | Porjai Rattanapanadda |
| collection | DOAJ |
| description | ObjectivesThe purpose of this study was to characterize the prevalence of antimicrobial resistance in Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from healthy feedlot cattle over 2 years, and investigate factors potentially associated with recovery of resistant isolates.MethodsDeep-guarded nasopharyngeal (NP) swabs were used to sample feedlot cattle in multiple randomly selected feedlots (2019 n = 21, 2020 n = 26) at 2 timepoints. NP swabs were collected from 16 animals in each enrolled group upon entry processing and later in the feeding period. Cattle from the same groups (not necessarily the same animals) were sampled at both timepoints. Susceptibility testing was performed using the broth microdilution.ResultsA total of 1,392 cattle within 47 housing groups were sampled over 2 years, providing 625 bacterial isolates for investigation. Pasteurella multocida (27.4%) was the most frequently isolated BRD organism, followed by H. somni (9%) and M. haemolytica (8.5%). Resistance to ≥3 antimicrobial classes was detected in 2.4% of M. haemolytica, 3.4% of H. somni, and 21.3% of P. multocida isolates. Potential associations were investigated between recovery of resistant organisms and time of year at sampling (quarter), sampling timepoint (arrival or second sample), days on feed (DOF) at sampling, animal age categories, and BRD risk categories. There was a significant (p < 0.05) increase in resistance prevalence after arrival for macrolide drugs in M. haemolytica, and for ampicillin, danofloxacin, enrofloxacin, spectinomycin, gamithromycin, tildipirosin, tulathromycin and tetracycline in P. multocida isolates. Resistance was higher in calves than in yearlings for tulathromycin in H. somni, and for gamithromycin, spectinomycin, tulathromycin, tildipirosin, and tetracycline for P. multocida (p < 0.05) Resistance to tetracycline, tildipirosin, and tulathromycin decreased between 61–80 DOF and 81–100 DOF when compared to 20–40 DOF, whereas for spectinomycin, resistance was lower in cattle sampled between 61–80 DOF than those sampled at 20–40 DOF for P. multocida.DiscussionThe diversity of AMR profiles and associated risk factors between the BRD pathogens studied, underscores the importance of including all three organisms in future AMR studies in beef cattle. |
| format | Article |
| id | doaj-art-611200ddad6b4fbbb8077958dc3381d4 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-611200ddad6b4fbbb8077958dc3381d42025-01-28T06:41:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.14974021497402The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in CanadaPorjai Rattanapanadda0Porjai Rattanapanadda1Dana Ramsay2Alyssa Butters3Calvin W. Booker4Sherry J. Hannon5Steve Hendrick6Joyce Van Donkersgoed7Brian N. Warr8Sheryl P. Gow9Paul S. Morley10Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanDepartment of Livestock Development, Ministry of Agriculture and Cooperatives, Bangkok, ThailandDepartment of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, SK, CanadaFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, CanadaTelus Agriculture, Okotoks, AB, CanadaTelus Agriculture, Okotoks, AB, CanadaCoaldale Veterinary Clinic, Lethbridge, AB, CanadaDr. Joyce Van Donkersgoed Inc., Coaldale, AB, CanadaVeterinary Agri Health Services, Rocky View, AB, CanadaCanadian Integrated Program for Antimicrobial Resistance Surveillance, Public Health Agency of Canada, Saskatoon, SK, Canada0Veterinary Education, Research and Outreach (VERO) Program, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, Canyon, TX, United StatesObjectivesThe purpose of this study was to characterize the prevalence of antimicrobial resistance in Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from healthy feedlot cattle over 2 years, and investigate factors potentially associated with recovery of resistant isolates.MethodsDeep-guarded nasopharyngeal (NP) swabs were used to sample feedlot cattle in multiple randomly selected feedlots (2019 n = 21, 2020 n = 26) at 2 timepoints. NP swabs were collected from 16 animals in each enrolled group upon entry processing and later in the feeding period. Cattle from the same groups (not necessarily the same animals) were sampled at both timepoints. Susceptibility testing was performed using the broth microdilution.ResultsA total of 1,392 cattle within 47 housing groups were sampled over 2 years, providing 625 bacterial isolates for investigation. Pasteurella multocida (27.4%) was the most frequently isolated BRD organism, followed by H. somni (9%) and M. haemolytica (8.5%). Resistance to ≥3 antimicrobial classes was detected in 2.4% of M. haemolytica, 3.4% of H. somni, and 21.3% of P. multocida isolates. Potential associations were investigated between recovery of resistant organisms and time of year at sampling (quarter), sampling timepoint (arrival or second sample), days on feed (DOF) at sampling, animal age categories, and BRD risk categories. There was a significant (p < 0.05) increase in resistance prevalence after arrival for macrolide drugs in M. haemolytica, and for ampicillin, danofloxacin, enrofloxacin, spectinomycin, gamithromycin, tildipirosin, tulathromycin and tetracycline in P. multocida isolates. Resistance was higher in calves than in yearlings for tulathromycin in H. somni, and for gamithromycin, spectinomycin, tulathromycin, tildipirosin, and tetracycline for P. multocida (p < 0.05) Resistance to tetracycline, tildipirosin, and tulathromycin decreased between 61–80 DOF and 81–100 DOF when compared to 20–40 DOF, whereas for spectinomycin, resistance was lower in cattle sampled between 61–80 DOF than those sampled at 20–40 DOF for P. multocida.DiscussionThe diversity of AMR profiles and associated risk factors between the BRD pathogens studied, underscores the importance of including all three organisms in future AMR studies in beef cattle.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1497402/fullbovine respiratory diseaseantimicrobial resistanceMannheimia haemolyticaPasteurella multocidaHistophilus somni |
| spellingShingle | Porjai Rattanapanadda Porjai Rattanapanadda Dana Ramsay Alyssa Butters Calvin W. Booker Sherry J. Hannon Steve Hendrick Joyce Van Donkersgoed Brian N. Warr Sheryl P. Gow Paul S. Morley The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada Frontiers in Microbiology bovine respiratory disease antimicrobial resistance Mannheimia haemolytica Pasteurella multocida Histophilus somni |
| title | The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada |
| title_full | The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada |
| title_fullStr | The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada |
| title_full_unstemmed | The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada |
| title_short | The prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in Canada |
| title_sort | prevalence and antimicrobial resistance of respiratory pathogens isolated from feedlot cattle in canada |
| topic | bovine respiratory disease antimicrobial resistance Mannheimia haemolytica Pasteurella multocida Histophilus somni |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1497402/full |
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