Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization
Purpose: This study elucidates the mechanism of the physiological effect of cannabidiol (CBD) by assessing its impact on lipopolysaccharide (LPS)-induced inflammation in RWPE-1 cells and prostatitis-induced by 17β-estradiol and dihydrotestosterone in a rat model, focusing on its therapeutic potent...
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Korean Society for Sexual Medicine and Andrology
2025-01-01
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| Series: | The World Journal of Men's Health |
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| author | Jun Jie Piao Soomin Kim Dongho Shin Hwa Jong Lee Kyung-Hwa Jeon Wen Jie Tian Kyung Jae Hur Jong Soo Kang Hyun-Je Park Joo Young Cha Aeri Song Sang-Hyuck Park Mahadevan Rajasekaran Woong Jin Bae Sungjoo Kim Yoon Sae Woong Kim |
| author_facet | Jun Jie Piao Soomin Kim Dongho Shin Hwa Jong Lee Kyung-Hwa Jeon Wen Jie Tian Kyung Jae Hur Jong Soo Kang Hyun-Je Park Joo Young Cha Aeri Song Sang-Hyuck Park Mahadevan Rajasekaran Woong Jin Bae Sungjoo Kim Yoon Sae Woong Kim |
| author_sort | Jun Jie Piao |
| collection | DOAJ |
| description | Purpose: This study elucidates the mechanism of the physiological effect of cannabidiol (CBD) by assessing its impact on lipopolysaccharide
(LPS)-induced inflammation in RWPE-1 cells and prostatitis-induced by 17β-estradiol and dihydrotestosterone
in a rat model, focusing on its therapeutic potential for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).
Materials and Methods: RWPE-1 cells were stratified in vitro into three groups: (1) controls, (2) cells with LPS-induced inflammation,
and (3) cells with LPS-induced inflammation and treated with CBD. Enzyme-linked immunosorbent assays and western
blots were performed on cellular components and supernatants after administration of CBD. Five groups of six Sprague–
Dawley male rats were assigned: (1) control, (2) CP/CPPS, (3) CP/CPPS and treated with 50 mg/kg CBD, (4) CP/CPPS and
treated with 100 mg/kg CBD, and (5) CP/CPPS and treated with 150 mg/kg CBD. Prostatitis was induced through administration
of 17β-estradiol and dihydrotestosterone. After four weeks of CBD treatment, a pain index was evaluated, and prostate
tissue was collected for subsequent histologic examination and western blot analysis.
Results: CBD demonstrated efficacy in vivo for CP/CPPS and in vitro for inflammation. It inhibited the toll-like receptor 4
(TLR4)/nuclear factor-kappa B (NF-κB) pathway by activating the CB2 receptor, reducing expression of interleukin-6, tumor
necrosis factor-alpha, and cyclooxygenase-2 (COX2) (p<0.01). CBD exhibited analgesic effects by activating and desensitizing
the TRPV1 receptor.
Conclusions: CBD inhibits the TLR4/NF-κB pathway by activating the CB2 receptor, desensitizes the TRPV1 receptor, and
decreases the release of COX2. This results in relief of inflammation and pain in patients with CP/CPPS, indicating CBD as a
potential treatment for CP/CPPS. |
| format | Article |
| id | doaj-art-61089c63b778456d901a75fd93d667f8 |
| institution | Kabale University |
| issn | 2287-4208 2287-4690 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj-art-61089c63b778456d901a75fd93d667f82025-01-03T06:42:46ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902025-01-0143122823810.5534/wjmh.230352Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 DesensitizationJun Jie Piao0https://orcid.org/0000-0002-8223-7487Soomin Kim1https://orcid.org/0000-0003-4834-9941Dongho Shin2https://orcid.org/0000-0001-8496-8825Hwa Jong Lee3https://orcid.org/0000-0002-0907-6533Kyung-Hwa Jeon4https://orcid.org/0009-0000-1201-6956Wen Jie Tian5https://orcid.org/0000-0002-6037-1319Kyung Jae Hur6https://orcid.org/0000-0002-4080-5466Jong Soo Kang7https://orcid.org/0000-0002-0172-0021Hyun-Je Park8https://orcid.org/0009-0008-3632-947XJoo Young Cha9https://orcid.org/0000-0002-3679-9059Aeri Song10https://orcid.org/0000-0003-4541-3004Sang-Hyuck Park11https://orcid.org/0000-0003-3611-8011Mahadevan Rajasekaran12https://orcid.org/0000-0003-4188-1628Woong Jin Bae13https://orcid.org/0000-0001-7703-1161Sungjoo Kim Yoon14https://orcid.org/0000-0003-2497-729XSae Woong Kim15https://orcid.org/0000-0002-9127-9400Department of Urology, College of Medicine, The Catholic University of Korea, SeoulDepartment of Urology, College of Medicine, The Catholic University of Korea, SeoulDepartment of Urology, Catholic Kwandong University College of Medicine, IncheonGreen Medicine Co., Ltd, BusanCollege of Pharmacy, Ewha Womans University, SeoulDepartment of Urology, The Second Hospital of Jilin University, ChangchunDepartment of Urology, Daegu Fatima Hospital, DaeguYuhan Care Co., Ltd., SeoulYuhan Natural Product R&D Center, Yuhan Care Co., Ltd., YonginYuhan Natural Product R&D Center, Yuhan Care Co., Ltd., YonginYuhan Natural Product R&D Center, Yuhan Care Co., Ltd., YonginInstitute of Cannabis Research, Colorado State University–Pueblo, Pueblo, CODepartment of Urology, San Diego VA Health Care System & University of California, San Diego, CADepartment of Urology, College of Medicine, The Catholic University of Korea, SeoulDepartment of Biomedicine & Health Sciences, The Catholic University of Korea, SeoulDepartment of Urology, College of Medicine, The Catholic University of Korea, SeoulPurpose: This study elucidates the mechanism of the physiological effect of cannabidiol (CBD) by assessing its impact on lipopolysaccharide (LPS)-induced inflammation in RWPE-1 cells and prostatitis-induced by 17β-estradiol and dihydrotestosterone in a rat model, focusing on its therapeutic potential for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Materials and Methods: RWPE-1 cells were stratified in vitro into three groups: (1) controls, (2) cells with LPS-induced inflammation, and (3) cells with LPS-induced inflammation and treated with CBD. Enzyme-linked immunosorbent assays and western blots were performed on cellular components and supernatants after administration of CBD. Five groups of six Sprague– Dawley male rats were assigned: (1) control, (2) CP/CPPS, (3) CP/CPPS and treated with 50 mg/kg CBD, (4) CP/CPPS and treated with 100 mg/kg CBD, and (5) CP/CPPS and treated with 150 mg/kg CBD. Prostatitis was induced through administration of 17β-estradiol and dihydrotestosterone. After four weeks of CBD treatment, a pain index was evaluated, and prostate tissue was collected for subsequent histologic examination and western blot analysis. Results: CBD demonstrated efficacy in vivo for CP/CPPS and in vitro for inflammation. It inhibited the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway by activating the CB2 receptor, reducing expression of interleukin-6, tumor necrosis factor-alpha, and cyclooxygenase-2 (COX2) (p<0.01). CBD exhibited analgesic effects by activating and desensitizing the TRPV1 receptor. Conclusions: CBD inhibits the TLR4/NF-κB pathway by activating the CB2 receptor, desensitizes the TRPV1 receptor, and decreases the release of COX2. This results in relief of inflammation and pain in patients with CP/CPPS, indicating CBD as a potential treatment for CP/CPPS.cannabidiolprostatitisreceptor; cannabinoid; cb2toll-like receptor 4trpv1 receptor |
| spellingShingle | Jun Jie Piao Soomin Kim Dongho Shin Hwa Jong Lee Kyung-Hwa Jeon Wen Jie Tian Kyung Jae Hur Jong Soo Kang Hyun-Je Park Joo Young Cha Aeri Song Sang-Hyuck Park Mahadevan Rajasekaran Woong Jin Bae Sungjoo Kim Yoon Sae Woong Kim Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization The World Journal of Men's Health cannabidiol prostatitis receptor; cannabinoid; cb2 toll-like receptor 4 trpv1 receptor |
| title | Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization |
| title_full | Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization |
| title_fullStr | Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization |
| title_full_unstemmed | Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization |
| title_short | Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization |
| title_sort | cannabidiol alleviates chronic prostatitis and chronic pelvic pain syndrome via cb2 receptor activation and trpv1 desensitization |
| topic | cannabidiol prostatitis receptor; cannabinoid; cb2 toll-like receptor 4 trpv1 receptor |
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