Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam
In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more acc...
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University of Brawijaya
2019-02-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/308 |
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| author | Fitri Sil Valen Maheno Sri Widodo Yuni Kilawati |
| author_facet | Fitri Sil Valen Maheno Sri Widodo Yuni Kilawati |
| author_sort | Fitri Sil Valen |
| collection | DOAJ |
| description | In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%. |
| format | Article |
| id | doaj-art-60ffab1d404c455ca9b640e6b3ef8810 |
| institution | OA Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2019-02-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-60ffab1d404c455ca9b640e6b3ef88102025-08-20T01:50:07ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552019-02-01914046https://doi.org/10.21776/ub.jels.2019.009.01.07Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh DamFitri Sil Valen0Maheno Sri Widodo1Yuni Kilawati2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaIn general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%.https://jels.ub.ac.id/index.php/jels/article/view/308dna barcodemolecular identificationmystacoleucusphylogenetic coi. |
| spellingShingle | Fitri Sil Valen Maheno Sri Widodo Yuni Kilawati Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam Journal of Experimental Life Science dna barcode molecular identification mystacoleucus phylogenetic coi. |
| title | Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam |
| title_full | Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam |
| title_fullStr | Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam |
| title_full_unstemmed | Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam |
| title_short | Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam |
| title_sort | molecular identification and genetic characteristics of genus mystacoleucus based on gene cytochrome oxidase c subunit i coi in sengguruh dam |
| topic | dna barcode molecular identification mystacoleucus phylogenetic coi. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/308 |
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