Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells
This work is dedicated to the study of the photoluminescence kinetics of aluminum phthalocyanine nanoparticles in colloidal solutions at different pH and in the interaction with immune cells (macrophages). For measurements we used a registration system based on Hamamatsu streak camera (C10627-13 Ham...
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| Format: | Article |
| Language: | Russian |
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Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"
2016-04-01
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| Series: | Biomedical Photonics |
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| Online Access: | https://www.pdt-journal.com/jour/article/view/81 |
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| author | F. G. Bystrov V. I. Makarov D. V. Pominova A. V. Ryabova V. B. Loschenov |
| author_facet | F. G. Bystrov V. I. Makarov D. V. Pominova A. V. Ryabova V. B. Loschenov |
| author_sort | F. G. Bystrov |
| collection | DOAJ |
| description | This work is dedicated to the study of the photoluminescence kinetics of aluminum phthalocyanine nanoparticles in colloidal solutions at different pH and in the interaction with immune cells (macrophages). For measurements we used a registration system based on Hamamatsu streak camera (C10627-13 Hamamatsu Photonics) with picosecond temporal resolution (15 ps), conjugated with the fiberoptic spectrometer and picosecond laser pumping. The changes in fluorescence decay kinetics as additional lifetime components of fluorescence were found during the experiment. The number of components and duration of lifetimes changed while interacting with cells and depends on pH. At pH 2 the presence of two fluorescence lifetimes was recorded: the first one was 5 ns, which corresponded to the molecular form in solution, and 1.5 ns, which corresponded to bound state of phthalocyanine molecules. Due to the absence of other possible objects for bounding in the solution except of the nanoparticles we can suggest with a high degree of accuracy that the bounding occurs with the very these nanoparticles. Analysis of the fluorescence lifetimes of aluminum phthalocyanine nanoparticles in macrophages indicated the presence of two components: 9 ns and 4.5 ns. A model of surface molecules transitions from parallel to perpendicular position, regarding to the plane of the crystal nanoparticle was proposed. |
| format | Article |
| id | doaj-art-60fc7dab1645402cbef6450687fb6d49 |
| institution | DOAJ |
| issn | 2413-9432 |
| language | Russian |
| publishDate | 2016-04-01 |
| publisher | Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association" |
| record_format | Article |
| series | Biomedical Photonics |
| spelling | doaj-art-60fc7dab1645402cbef6450687fb6d492025-08-20T03:21:34ZrusNon-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"Biomedical Photonics2413-94322016-04-01513810.24931/2413-9432-2016-5-1-3-879Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cellsF. G. Bystrov0V. I. Makarov1D. V. Pominova2A. V. Ryabova3V. B. Loschenov4National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, RussiaGeneral Physics Institute of the Russian Academy of Sciences, Moscow, RussiaGeneral Physics Institute of the Russian Academy of Sciences, Moscow, RussiaNational Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russia General Physics Institute of the Russian Academy of Sciences, Moscow, RussiaNational Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russia General Physics Institute of the Russian Academy of Sciences, Moscow, RussiaThis work is dedicated to the study of the photoluminescence kinetics of aluminum phthalocyanine nanoparticles in colloidal solutions at different pH and in the interaction with immune cells (macrophages). For measurements we used a registration system based on Hamamatsu streak camera (C10627-13 Hamamatsu Photonics) with picosecond temporal resolution (15 ps), conjugated with the fiberoptic spectrometer and picosecond laser pumping. The changes in fluorescence decay kinetics as additional lifetime components of fluorescence were found during the experiment. The number of components and duration of lifetimes changed while interacting with cells and depends on pH. At pH 2 the presence of two fluorescence lifetimes was recorded: the first one was 5 ns, which corresponded to the molecular form in solution, and 1.5 ns, which corresponded to bound state of phthalocyanine molecules. Due to the absence of other possible objects for bounding in the solution except of the nanoparticles we can suggest with a high degree of accuracy that the bounding occurs with the very these nanoparticles. Analysis of the fluorescence lifetimes of aluminum phthalocyanine nanoparticles in macrophages indicated the presence of two components: 9 ns and 4.5 ns. A model of surface molecules transitions from parallel to perpendicular position, regarding to the plane of the crystal nanoparticle was proposed.https://www.pdt-journal.com/jour/article/view/81aluminum phthalocyaninephotoluminescencenanoparticlesimmune cells. |
| spellingShingle | F. G. Bystrov V. I. Makarov D. V. Pominova A. V. Ryabova V. B. Loschenov Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells Biomedical Photonics aluminum phthalocyanine photoluminescence nanoparticles immune cells. |
| title | Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells |
| title_full | Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells |
| title_fullStr | Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells |
| title_full_unstemmed | Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells |
| title_short | Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells |
| title_sort | analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells |
| topic | aluminum phthalocyanine photoluminescence nanoparticles immune cells. |
| url | https://www.pdt-journal.com/jour/article/view/81 |
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