Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains?
Specific determinants associated with Uropathogenic Escherichia coli (UPEC) causing recurrent cystitis are still poorly characterized. Using strains from a previous clinical study (Vitale study, clinicaltrials.gov, identifier NCT02292160) the aims of this study were (i) to describe genomic and pheno...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2024.2444689 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850050766256996352 |
|---|---|
| author | Nicolas Vautrin Sandrine Dahyot Marie Leoz François Caron Maxime Grand Audrey Feldmann François Gravey Stéphanie Legris David Ribet Kévin Alexandre Martine Pestel-Caron |
| author_facet | Nicolas Vautrin Sandrine Dahyot Marie Leoz François Caron Maxime Grand Audrey Feldmann François Gravey Stéphanie Legris David Ribet Kévin Alexandre Martine Pestel-Caron |
| author_sort | Nicolas Vautrin |
| collection | DOAJ |
| description | Specific determinants associated with Uropathogenic Escherichia coli (UPEC) causing recurrent cystitis are still poorly characterized. Using strains from a previous clinical study (Vitale study, clinicaltrials.gov, identifier NCT02292160) the aims of this study were (i) to describe genomic and phenotypic traits associated with recurrence using a large collection of recurrent and paired sporadic UPEC isolates and (ii) to explore within-host genomic adaptation associated with recurrence using series of 2 to 5 sequential UPEC isolates. Whole genome comparative analyses between 24 recurrent cystitis isolates (RCIs) and 24 phylogenetically paired sporadic cystitis isolates (SCIs) suggested a lower prevalence of putative mobile genetic elements (MGE) in RCIs, such as plasmids and prophages. The intra-patient evolution of the 24 RCI series over time was characterized by SNP occurrence in genes involved in metabolism or membrane transport and by plasmid loss in 5 out of the 24 RCI series. Genomic evolution occurred early in the course of recurrence, suggesting rapid adaptation to strong selection pressure in the urinary tract. However, RCIs did not exhibit specific virulence factor determinants and could not be distinguished from SCIs by their fitness, biofilm formation, or ability to invade HTB-9 bladder epithelial cells. Taken together, these results suggest a rapid but not convergent adaptation of RCIs that involves both strain- and host-specific characteristics. |
| format | Article |
| id | doaj-art-60f5da4dcdf54c60952294858e57102f |
| institution | DOAJ |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-60f5da4dcdf54c60952294858e57102f2025-08-20T02:53:21ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2024.2444689Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains?Nicolas Vautrin0Sandrine Dahyot1Marie Leoz2François Caron3Maxime Grand4Audrey Feldmann5François Gravey6Stéphanie Legris7David Ribet8Kévin Alexandre9Martine Pestel-Caron10Univ Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, Rouen, FranceDepartment of Microbiology, Univ Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, CHU Rouen, Rouen, FranceUniv Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, Rouen, FranceDepartment of Infectious Diseases, Univ Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, CHU Rouen, Rouen, FranceUniv Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, Rouen, FranceUniv Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, Rouen, FranceUniversité de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, Caen, FranceUniv Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, Rouen, FranceUniv Rouen Normandie, INSERM, Normandie Univ, ADEN UMR 1073, Nutrition, Inflammation and Microbiota-Gut-Brain, Axis, FranceDepartment of Infectious Diseases, Univ Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, CHU Rouen, Rouen, FranceDepartment of Infectious Diseases, Univ Rouen Normandie, Université de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, CHU Rouen, Rouen, FranceSpecific determinants associated with Uropathogenic Escherichia coli (UPEC) causing recurrent cystitis are still poorly characterized. Using strains from a previous clinical study (Vitale study, clinicaltrials.gov, identifier NCT02292160) the aims of this study were (i) to describe genomic and phenotypic traits associated with recurrence using a large collection of recurrent and paired sporadic UPEC isolates and (ii) to explore within-host genomic adaptation associated with recurrence using series of 2 to 5 sequential UPEC isolates. Whole genome comparative analyses between 24 recurrent cystitis isolates (RCIs) and 24 phylogenetically paired sporadic cystitis isolates (SCIs) suggested a lower prevalence of putative mobile genetic elements (MGE) in RCIs, such as plasmids and prophages. The intra-patient evolution of the 24 RCI series over time was characterized by SNP occurrence in genes involved in metabolism or membrane transport and by plasmid loss in 5 out of the 24 RCI series. Genomic evolution occurred early in the course of recurrence, suggesting rapid adaptation to strong selection pressure in the urinary tract. However, RCIs did not exhibit specific virulence factor determinants and could not be distinguished from SCIs by their fitness, biofilm formation, or ability to invade HTB-9 bladder epithelial cells. Taken together, these results suggest a rapid but not convergent adaptation of RCIs that involves both strain- and host-specific characteristics.https://www.tandfonline.com/doi/10.1080/21505594.2024.2444689Uropathogenic Escherichia colirecurrent cystitisurinary tract infectionsvirulence factorswhole genome sequencinggenome-wide association studies |
| spellingShingle | Nicolas Vautrin Sandrine Dahyot Marie Leoz François Caron Maxime Grand Audrey Feldmann François Gravey Stéphanie Legris David Ribet Kévin Alexandre Martine Pestel-Caron Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains? Virulence Uropathogenic Escherichia coli recurrent cystitis urinary tract infections virulence factors whole genome sequencing genome-wide association studies |
| title | Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains? |
| title_full | Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains? |
| title_fullStr | Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains? |
| title_full_unstemmed | Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains? |
| title_short | Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains? |
| title_sort | are escherichia coli causing recurrent cystitis just ordinary uropathogenic e coli upec strains |
| topic | Uropathogenic Escherichia coli recurrent cystitis urinary tract infections virulence factors whole genome sequencing genome-wide association studies |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2024.2444689 |
| work_keys_str_mv | AT nicolasvautrin areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT sandrinedahyot areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT marieleoz areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT francoiscaron areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT maximegrand areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT audreyfeldmann areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT francoisgravey areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT stephanielegris areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT davidribet areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT kevinalexandre areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains AT martinepestelcaron areescherichiacolicausingrecurrentcystitisjustordinaryuropathogenicecoliupecstrains |