The effect of Prrx2 on the development of mouse molar germ
[Objective:] To investigate the expression level of paired related homeobox 2 (Prrx2) at different stages of mouse molar germ and its effect on molar germ development. [Methods:] Real‐time quantitative polymerase chain reaction (RT‐qPCR) was used to analyze the relative expression of paired related...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-06-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.001 |
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| _version_ | 1849225101373865984 |
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| author | ZHU Zhewen HAN Xue LIU Liwei WANG Zuolin |
| author_facet | ZHU Zhewen HAN Xue LIU Liwei WANG Zuolin |
| author_sort | ZHU Zhewen |
| collection | DOAJ |
| description | [Objective:] To investigate the expression level of paired related homeobox 2 (Prrx2) at different stages of mouse molar germ and its effect on molar germ development. [Methods:] Real‐time quantitative polymerase chain reaction (RT‐qPCR) was used to analyze the relative expression of paired related homeobox 1 (Prrx1) and Prrx2 in E12.5 to E18.5, P1, P3, P7, and E13.5 molar germ, hair, brain, heart, colon, kidney, lung, salivary gland and other tissues; small interfering RNA (siRNA) was used to silence the expression of Prrx2, then tooth germ development was observed via vitro culture systems; CCK8 test and cell scratch test were utilized to observe the proliferation and migration ability of molar germ mesenchymal cells. [Results:] According to the results of RT‐qPCR, comparing with Prrx2 in other organs, it was highly expressed in the molars, hair and salivary glands of E13.5 mice; comparing with Prrx1, the expression level of Prrx2 at the late stage of tooth germ development (E16.5‐E18.5) is still higher than that at the initial stage of development (E13.5); the results of in vitro tooth germ culture demonstrated that after silencing Prrx2, tooth germ width, height and tooth cusp height were reduced by (21.11±3.49)%, (12.08±5.30)% and (22.34±7.56)% (all P<0.05), respectively; CCK8 test and cell scratch test showed that the proliferation rate of tooth germ mesenchymal cells did not change after silencing Prrx2 (P>0.05), but the migration ability was suppressed (P<0.05). [Conclusion:] Prrx2 plays an important role in the process of molar germ morphogenesis; Prrx2 affects the growth of molar germ and the process of tooth cusp development via promoting the migration ability of molar germ mesenchymal cells. |
| format | Article |
| id | doaj-art-60f28c8e23944e528e2f7833744a1d18 |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2023-06-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-60f28c8e23944e528e2f7833744a1d182025-08-25T06:10:06ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792023-06-0133313113710.3969/j.issn.1005-4979.2023.03.001The effect of Prrx2 on the development of mouse molar germZHU Zhewen0HAN Xue1LIU Liwei2WANG Zuolin3Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Oral Implantology, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Oral Implantology, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Oral Implantology, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Oral Implantology, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China[Objective:] To investigate the expression level of paired related homeobox 2 (Prrx2) at different stages of mouse molar germ and its effect on molar germ development. [Methods:] Real‐time quantitative polymerase chain reaction (RT‐qPCR) was used to analyze the relative expression of paired related homeobox 1 (Prrx1) and Prrx2 in E12.5 to E18.5, P1, P3, P7, and E13.5 molar germ, hair, brain, heart, colon, kidney, lung, salivary gland and other tissues; small interfering RNA (siRNA) was used to silence the expression of Prrx2, then tooth germ development was observed via vitro culture systems; CCK8 test and cell scratch test were utilized to observe the proliferation and migration ability of molar germ mesenchymal cells. [Results:] According to the results of RT‐qPCR, comparing with Prrx2 in other organs, it was highly expressed in the molars, hair and salivary glands of E13.5 mice; comparing with Prrx1, the expression level of Prrx2 at the late stage of tooth germ development (E16.5‐E18.5) is still higher than that at the initial stage of development (E13.5); the results of in vitro tooth germ culture demonstrated that after silencing Prrx2, tooth germ width, height and tooth cusp height were reduced by (21.11±3.49)%, (12.08±5.30)% and (22.34±7.56)% (all P<0.05), respectively; CCK8 test and cell scratch test showed that the proliferation rate of tooth germ mesenchymal cells did not change after silencing Prrx2 (P>0.05), but the migration ability was suppressed (P<0.05). [Conclusion:] Prrx2 plays an important role in the process of molar germ morphogenesis; Prrx2 affects the growth of molar germ and the process of tooth cusp development via promoting the migration ability of molar germ mesenchymal cells.https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.001tooth germ developmentprrx2in vitro culturemice |
| spellingShingle | ZHU Zhewen HAN Xue LIU Liwei WANG Zuolin The effect of Prrx2 on the development of mouse molar germ Kouqiang hemian waike zazhi tooth germ development prrx2 in vitro culture mice |
| title | The effect of Prrx2 on the development of mouse molar germ |
| title_full | The effect of Prrx2 on the development of mouse molar germ |
| title_fullStr | The effect of Prrx2 on the development of mouse molar germ |
| title_full_unstemmed | The effect of Prrx2 on the development of mouse molar germ |
| title_short | The effect of Prrx2 on the development of mouse molar germ |
| title_sort | effect of prrx2 on the development of mouse molar germ |
| topic | tooth germ development prrx2 in vitro culture mice |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.001 |
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