The effect of Prrx2 on the development of mouse molar germ
[Objective:] To investigate the expression level of paired related homeobox 2 (Prrx2) at different stages of mouse molar germ and its effect on molar germ development. [Methods:] Real‐time quantitative polymerase chain reaction (RT‐qPCR) was used to analyze the relative expression of paired related...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-06-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.001 |
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| Summary: | [Objective:] To investigate the expression level of paired related homeobox 2 (Prrx2) at different stages of mouse molar germ and its effect on molar germ development. [Methods:] Real‐time quantitative polymerase chain reaction (RT‐qPCR) was used to analyze the relative expression of paired related homeobox 1 (Prrx1) and Prrx2 in E12.5 to E18.5, P1, P3, P7, and E13.5 molar germ, hair, brain, heart, colon, kidney, lung, salivary gland and other tissues; small interfering RNA (siRNA) was used to silence the expression of Prrx2, then tooth germ development was observed via vitro culture systems; CCK8 test and cell scratch test were utilized to observe the proliferation and migration ability of molar germ mesenchymal cells. [Results:] According to the results of RT‐qPCR, comparing with Prrx2 in other organs, it was highly expressed in the molars, hair and salivary glands of E13.5 mice; comparing with Prrx1, the expression level of Prrx2 at the late stage of tooth germ development (E16.5‐E18.5) is still higher than that at the initial stage of development (E13.5); the results of in vitro tooth germ culture demonstrated that after silencing Prrx2, tooth germ width, height and tooth cusp height were reduced by (21.11±3.49)%, (12.08±5.30)% and (22.34±7.56)% (all P<0.05), respectively; CCK8 test and cell scratch test showed that the proliferation rate of tooth germ mesenchymal cells did not change after silencing Prrx2 (P>0.05), but the migration ability was suppressed (P<0.05). [Conclusion:] Prrx2 plays an important role in the process of molar germ morphogenesis; Prrx2 affects the growth of molar germ and the process of tooth cusp development via promoting the migration ability of molar germ mesenchymal cells. |
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| ISSN: | 1005-4979 |