Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i>
Heat shock transcription factor (Hsf) plays a crucial role in the signal transduction pathways of plants in response to drought stress. However, studies exploring the specific functions and mechanisms of action of the Hsf family in tea plants (<i>Camellia sinensis</i> L.) remain limited....
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2025-03-01
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| author | Yuqi Luo Chenyu Shao Rong Xu Shuqi Qiu Qiulong Hu Jiayi Guo Yun Peng Han Tang Yueling Zhao Jianan Huang Zhonghua Liu Chengwen Shen |
| author_facet | Yuqi Luo Chenyu Shao Rong Xu Shuqi Qiu Qiulong Hu Jiayi Guo Yun Peng Han Tang Yueling Zhao Jianan Huang Zhonghua Liu Chengwen Shen |
| author_sort | Yuqi Luo |
| collection | DOAJ |
| description | Heat shock transcription factor (Hsf) plays a crucial role in the signal transduction pathways of plants in response to drought stress. However, studies exploring the specific functions and mechanisms of action of the Hsf family in tea plants (<i>Camellia sinensis</i> L.) remain limited. In this study, we identified 31 members of the CsHsf family from the <i>C. sinensis</i> genome. <i>CsHsf10</i> was determined to be a potential drought-resistant candidate gene by screening 10 highly expressed genes in mature leaves and confirming results through RT-qPCR. Correlation analysis indicates that <i>CsHsf10</i> may enhance the drought resistance of tea plants by participating in the tea polyphenol synthesis pathway and regulating the expression of antioxidant enzyme genes. Furthermore, overexpression experiments in <i>Arabidopsis</i> and antisense oligonucleotide experiments in tea plants corroborated that <i>CsHsf10</i> exerts a significant positive regulatory effect on drought resistance in tea plants. Yeast one-hybrid assays and dual luciferase reporter gene experiments demonstrated that <i>CsHsf10</i> can directly target <i>CsPOD17</i>, significantly promoting its transcriptional expression. Additionally, we found that the expression of <i>CsHsf10</i> contributes to the increased accumulation of catechin components in tea plants under drought stress. These findings suggest that, during the response of tea plants to drought stress, <i>CsHsf10</i> not only enhances antioxidant capacity by regulating the activity of antioxidant enzymes but also optimizes the physiological state of tea plants by influencing the accumulation of secondary metabolites, thereby significantly improving their drought resistance. |
| format | Article |
| id | doaj-art-60ec5257142242518af41b24cdefc309 |
| institution | DOAJ |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-60ec5257142242518af41b24cdefc3092025-08-20T03:13:31ZengMDPI AGHorticulturae2311-75242025-03-0111437310.3390/horticulturae11040373Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i>Yuqi Luo0Chenyu Shao1Rong Xu2Shuqi Qiu3Qiulong Hu4Jiayi Guo5Yun Peng6Han Tang7Yueling Zhao8Jianan Huang9Zhonghua Liu10Chengwen Shen11National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaYuelushan Laboratory, Changsha 410128, ChinaYuelushan Laboratory, Changsha 410128, ChinaTea Research Institute, Shaoyang Academy of Agricultural Sciences, Shaoyang 422000, ChinaDepartment of TeaScience, Sichuan Agricultural University, Chengdu 611130, ChinaNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Changsha 410128, ChinaHeat shock transcription factor (Hsf) plays a crucial role in the signal transduction pathways of plants in response to drought stress. However, studies exploring the specific functions and mechanisms of action of the Hsf family in tea plants (<i>Camellia sinensis</i> L.) remain limited. In this study, we identified 31 members of the CsHsf family from the <i>C. sinensis</i> genome. <i>CsHsf10</i> was determined to be a potential drought-resistant candidate gene by screening 10 highly expressed genes in mature leaves and confirming results through RT-qPCR. Correlation analysis indicates that <i>CsHsf10</i> may enhance the drought resistance of tea plants by participating in the tea polyphenol synthesis pathway and regulating the expression of antioxidant enzyme genes. Furthermore, overexpression experiments in <i>Arabidopsis</i> and antisense oligonucleotide experiments in tea plants corroborated that <i>CsHsf10</i> exerts a significant positive regulatory effect on drought resistance in tea plants. Yeast one-hybrid assays and dual luciferase reporter gene experiments demonstrated that <i>CsHsf10</i> can directly target <i>CsPOD17</i>, significantly promoting its transcriptional expression. Additionally, we found that the expression of <i>CsHsf10</i> contributes to the increased accumulation of catechin components in tea plants under drought stress. These findings suggest that, during the response of tea plants to drought stress, <i>CsHsf10</i> not only enhances antioxidant capacity by regulating the activity of antioxidant enzymes but also optimizes the physiological state of tea plants by influencing the accumulation of secondary metabolites, thereby significantly improving their drought resistance.https://www.mdpi.com/2311-7524/11/4/373<i>camellia sinensis</i>molecular breedingHsfdrought stressPOD |
| spellingShingle | Yuqi Luo Chenyu Shao Rong Xu Shuqi Qiu Qiulong Hu Jiayi Guo Yun Peng Han Tang Yueling Zhao Jianan Huang Zhonghua Liu Chengwen Shen Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i> Horticulturae <i>camellia sinensis</i> molecular breeding Hsf drought stress POD |
| title | Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i> |
| title_full | Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i> |
| title_fullStr | Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i> |
| title_full_unstemmed | Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i> |
| title_short | Functional Analysis of <i>CsHsf10</i> in Drought Stress Response in <i>Camellia sinensis</i> |
| title_sort | functional analysis of i cshsf10 i in drought stress response in i camellia sinensis i |
| topic | <i>camellia sinensis</i> molecular breeding Hsf drought stress POD |
| url | https://www.mdpi.com/2311-7524/11/4/373 |
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