IL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancer

Background/Aim. Gastric cancer (GC) is fourth most frequent malignant tumor worldwide, frequently diagnosed at advanced stages with poor prognosis. The aim of study was to determine expression of interleukin (IL)-32, proinflammatory and angiogenic mediators in the tumor, peritumor and healthy tissue...

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Main Authors: Pavlović Mladen, Jurišević Milena, Gajović Nevena, Mitrović Slobodanka, Jovanović Milan, Radosavljević Gordana, Pantić Jelena, Radovanović Dragče, Arsenijević Nebojša, Jovanović Ivan
Format: Article
Language:English
Published: Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade 2020-01-01
Series:Vojnosanitetski Pregled
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Online Access:http://www.doiserbia.nb.rs/img/doi/0042-8450/2020/0042-84501800158P.pdf
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author Pavlović Mladen
Jurišević Milena
Gajović Nevena
Mitrović Slobodanka
Jovanović Milan
Radosavljević Gordana
Pantić Jelena
Radovanović Dragče
Arsenijević Nebojša
Jovanović Ivan
author_facet Pavlović Mladen
Jurišević Milena
Gajović Nevena
Mitrović Slobodanka
Jovanović Milan
Radosavljević Gordana
Pantić Jelena
Radovanović Dragče
Arsenijević Nebojša
Jovanović Ivan
author_sort Pavlović Mladen
collection DOAJ
description Background/Aim. Gastric cancer (GC) is fourth most frequent malignant tumor worldwide, frequently diagnosed at advanced stages with poor prognosis. The aim of study was to determine expression of interleukin (IL)-32, proinflammatory and angiogenic mediators in the tumor, peritumor and healthy tissue, in patients with intestinal gastric cancer and the relationship with the disease severity. Methods. The tissue samples of intestinal type of the tumor of 60 patients with GC were analyzed. Expression of IL-32, vascular endothelial growth factor (VEGF), IL-17 and CD31 were measured by immunohistochemistry. Results. IL-32, VEGF and IL-17 expression as well as microvascular density (MVD) were diminished in adjacent tumor tissues compared with the tumor ones. Further, more intense expression of IL-32 and VEGF and enhanced MVD were noticed in patients with severe (TNM stages III and IV) and more progressive GC (lymph vessel invasion). Conclusion. Higher expression of IL-32, VEGF and intense MVD in the tumor tissue of GC patients with detectable lymph vessel invasion may be considered as a sign of the tumor’s malignant progression. This indicates a protumorogenic and proangiogenic role of IL-32 in biology of intestinal type of gastric cancer.
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spelling doaj-art-60e7d0baa3c8474ab1f939a2a96e268d2025-08-20T02:35:18ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202020-01-0177881682510.2298/VSP180727158P0042-84501800158PIL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancerPavlović Mladen0Jurišević Milena1Gajović Nevena2Mitrović Slobodanka3Jovanović Milan4Radosavljević Gordana5Pantić Jelena6Radovanović Dragče7Arsenijević Nebojša8Jovanović Ivan9University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia + University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Pathology, Kragujevac, SerbiaMilitary Medical Academy, Department of Abdominal Surgery, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, SerbiaBackground/Aim. Gastric cancer (GC) is fourth most frequent malignant tumor worldwide, frequently diagnosed at advanced stages with poor prognosis. The aim of study was to determine expression of interleukin (IL)-32, proinflammatory and angiogenic mediators in the tumor, peritumor and healthy tissue, in patients with intestinal gastric cancer and the relationship with the disease severity. Methods. The tissue samples of intestinal type of the tumor of 60 patients with GC were analyzed. Expression of IL-32, vascular endothelial growth factor (VEGF), IL-17 and CD31 were measured by immunohistochemistry. Results. IL-32, VEGF and IL-17 expression as well as microvascular density (MVD) were diminished in adjacent tumor tissues compared with the tumor ones. Further, more intense expression of IL-32 and VEGF and enhanced MVD were noticed in patients with severe (TNM stages III and IV) and more progressive GC (lymph vessel invasion). Conclusion. Higher expression of IL-32, VEGF and intense MVD in the tumor tissue of GC patients with detectable lymph vessel invasion may be considered as a sign of the tumor’s malignant progression. This indicates a protumorogenic and proangiogenic role of IL-32 in biology of intestinal type of gastric cancer.http://www.doiserbia.nb.rs/img/doi/0042-8450/2020/0042-84501800158P.pdfstomach neoplasmsil 32 protein, humananti-allergic agentsseverity of illness indexvascular endothelial growth factorsimmunohistochemistry
spellingShingle Pavlović Mladen
Jurišević Milena
Gajović Nevena
Mitrović Slobodanka
Jovanović Milan
Radosavljević Gordana
Pantić Jelena
Radovanović Dragče
Arsenijević Nebojša
Jovanović Ivan
IL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancer
Vojnosanitetski Pregled
stomach neoplasms
il 32 protein, human
anti-allergic agents
severity of illness index
vascular endothelial growth factors
immunohistochemistry
title IL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancer
title_full IL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancer
title_fullStr IL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancer
title_full_unstemmed IL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancer
title_short IL-32 expression associated with lymph vessel invasion in intestinal type of gastric cancer
title_sort il 32 expression associated with lymph vessel invasion in intestinal type of gastric cancer
topic stomach neoplasms
il 32 protein, human
anti-allergic agents
severity of illness index
vascular endothelial growth factors
immunohistochemistry
url http://www.doiserbia.nb.rs/img/doi/0042-8450/2020/0042-84501800158P.pdf
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