Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response
Objectives. To investigate whether hUC-MSCs attenuated severe burn-induced ALI and the effects were based on TSG-6 secreted from hUC-MSCs. Method. A rat model was established and evaluated as follows: cytokine expression was measured by ELISA, and both inflammatory cell infiltration and lung injury...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/8661689 |
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| _version_ | 1850120614048694272 |
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| author | Xiaohong Hu Lingying Liu Yu Wang Yonghui Yu Zhongyuan Li Yanan Liu Jiake Chai |
| author_facet | Xiaohong Hu Lingying Liu Yu Wang Yonghui Yu Zhongyuan Li Yanan Liu Jiake Chai |
| author_sort | Xiaohong Hu |
| collection | DOAJ |
| description | Objectives. To investigate whether hUC-MSCs attenuated severe burn-induced ALI and the effects were based on TSG-6 secreted from hUC-MSCs. Method. A rat model was established and evaluated as follows: cytokine expression was measured by ELISA, and both inflammatory cell infiltration and lung injury were assessed by immunohistochemistry assay. Results. In vitro, TSG-6 levels in serum from the burn group were significantly increased compared with those from the sham group. In vivo, TSG-6 levels of lung tissues and serum in the burn+hUC-MSC group were significantly increased compared with those in the burn group. Both in lung tissues and in serum, increased levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) were remarkably decreased, but the anti-inflammatory cytokine IL-10 increased after hUC-MSC administration (p<0.05). These significant positive effects after hUC-MSC transplantation did not occur in the burn+siTSG-6 group. Conclusion. The intratracheal implantation of hUC-MSCs has been an effective treatment for severe burn-induced ALI via promoting TSG-6 secretion and inhibiting inflammatory reaction in lung tissue. |
| format | Article |
| id | doaj-art-60d6d9dd6f2548fcb41e522afeed16cb |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-60d6d9dd6f2548fcb41e522afeed16cb2025-08-20T02:35:19ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/8661689Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory ResponseXiaohong Hu0Lingying Liu1Yu Wang2Yonghui Yu3Zhongyuan Li4Yanan Liu5Jiake Chai6Department of Pediatrics in the Fourth Medical CenterDepartment of Nutrition in the Fourth Medical CenterDepartment of Pediatrics in the Fourth Medical CenterBeijing Engineering and Technology Research Center of Food AdditivesDepartment of Pediatrics in the Fourth Medical CenterDepartment of Pediatrics in the Fourth Medical CenterDepartment of Burn & Plastic Surgery in the Fourth Medical CenterObjectives. To investigate whether hUC-MSCs attenuated severe burn-induced ALI and the effects were based on TSG-6 secreted from hUC-MSCs. Method. A rat model was established and evaluated as follows: cytokine expression was measured by ELISA, and both inflammatory cell infiltration and lung injury were assessed by immunohistochemistry assay. Results. In vitro, TSG-6 levels in serum from the burn group were significantly increased compared with those from the sham group. In vivo, TSG-6 levels of lung tissues and serum in the burn+hUC-MSC group were significantly increased compared with those in the burn group. Both in lung tissues and in serum, increased levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) were remarkably decreased, but the anti-inflammatory cytokine IL-10 increased after hUC-MSC administration (p<0.05). These significant positive effects after hUC-MSC transplantation did not occur in the burn+siTSG-6 group. Conclusion. The intratracheal implantation of hUC-MSCs has been an effective treatment for severe burn-induced ALI via promoting TSG-6 secretion and inhibiting inflammatory reaction in lung tissue.http://dx.doi.org/10.1155/2022/8661689 |
| spellingShingle | Xiaohong Hu Lingying Liu Yu Wang Yonghui Yu Zhongyuan Li Yanan Liu Jiake Chai Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response Stem Cells International |
| title | Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response |
| title_full | Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response |
| title_fullStr | Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response |
| title_full_unstemmed | Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response |
| title_short | Human Umbilical Cord-Derived Mesenchymal Stem Cells Alleviate Acute Lung Injury Caused by Severe Burn via Secreting TSG-6 and Inhibiting Inflammatory Response |
| title_sort | human umbilical cord derived mesenchymal stem cells alleviate acute lung injury caused by severe burn via secreting tsg 6 and inhibiting inflammatory response |
| url | http://dx.doi.org/10.1155/2022/8661689 |
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