Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis
ObjectiveCervical cancer ranks among the most prevalent malignancies impacting women globally. Disulfidptosis represents a recently identified pathway of cellular demise, although its role in the context of cervical cancer is not well elucidated. This research investigates the significance of Disulf...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-05-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2025.1574911/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849322323842170880 |
|---|---|
| author | Yadan Yao Xiaomin Yang Yuanxin Fu Yinmin Zhang |
| author_facet | Yadan Yao Xiaomin Yang Yuanxin Fu Yinmin Zhang |
| author_sort | Yadan Yao |
| collection | DOAJ |
| description | ObjectiveCervical cancer ranks among the most prevalent malignancies impacting women globally. Disulfidptosis represents a recently identified pathway of cellular demise, although its role in the context of cervical cancer is not well elucidated. This research investigates the significance of Disulfidptosis-Related Genes (DRGs) within cervical cancer. Furthermore, it aims to analyze the differences in prognosis and immune infiltration among different molecular subtypes.MethodsWe compiled genes associated with cervical cancer and disulfidptosis from a variety of databases to perform a differential expression analysis. Subsequently, the samples are grouped through consensus clustering. To evaluate immune cell infiltration, we employed CIBERSORT. Additionally, immune checkpoint genes (ICGs) were gathered from existing literature and databases, enabling statistical analyses of two subtype samples of cervical cancer (CESC). Following our analyses using GO, KEGG, and GSEA to compare the differences between the two subtypes. Lastly, a prognostic risk model was constructed using LASSO regression and validated using ROC.ResultsThis study identified seven key genes: PCBP3, ARNT, ANP32E, DSTN, CD2AP, EPAS1, and ACTN1.The consensus clustering analysis showed differences in immune cell infiltration and DFS(disease-free survival) among the various clusters. The immune checkpoint gene CXCL1 displayed highly significant statistical differences between subtype A (Cluster 1) and subtype B (Cluster 2) in cervical cancer (CESC) samples. The gene set enrichment analysis identified the negative regulation of peptidase activity and the IL-17 signaling pathway, which link to subtype-specific differentially expressed genes (DEGs).ConclusionStatistical analysis of the various subtypes of CESC samples highlighted the importance of subtype-specific therapeutic targets. Additionally, it seeks to enhance the accuracy of prognostic predictions, thereby establishing a foundation for the formulation of personalized treatment approaches. |
| format | Article |
| id | doaj-art-60d4a36f234845bfb6412559c2a9a8a8 |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-60d4a36f234845bfb6412559c2a9a8a82025-08-20T03:49:26ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-05-011510.3389/fonc.2025.15749111574911Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosisYadan Yao0Xiaomin Yang1Yuanxin Fu2Yinmin Zhang3Department of Gynecology, Jiaxing University Affiliated Traditional Chinese Medicine (TCM) Hospital, Jiaxing, Zhejiang, ChinaDepartment of Gynecology, Jiaxing University Affiliated Traditional Chinese Medicine (TCM) Hospital, Jiaxing, Zhejiang, ChinaDepartment of Acupuncture and Massage, Jiaxing University Affiliated Traditional Chinese Medicine (TCM) Hospital, Jiaxing, Zhejiang, ChinaDepartment of Pediatrics, Jiaxing University Affiliated Traditional Chinese Medicine (TCM) Hospital, Jiaxing, Zhejiang, ChinaObjectiveCervical cancer ranks among the most prevalent malignancies impacting women globally. Disulfidptosis represents a recently identified pathway of cellular demise, although its role in the context of cervical cancer is not well elucidated. This research investigates the significance of Disulfidptosis-Related Genes (DRGs) within cervical cancer. Furthermore, it aims to analyze the differences in prognosis and immune infiltration among different molecular subtypes.MethodsWe compiled genes associated with cervical cancer and disulfidptosis from a variety of databases to perform a differential expression analysis. Subsequently, the samples are grouped through consensus clustering. To evaluate immune cell infiltration, we employed CIBERSORT. Additionally, immune checkpoint genes (ICGs) were gathered from existing literature and databases, enabling statistical analyses of two subtype samples of cervical cancer (CESC). Following our analyses using GO, KEGG, and GSEA to compare the differences between the two subtypes. Lastly, a prognostic risk model was constructed using LASSO regression and validated using ROC.ResultsThis study identified seven key genes: PCBP3, ARNT, ANP32E, DSTN, CD2AP, EPAS1, and ACTN1.The consensus clustering analysis showed differences in immune cell infiltration and DFS(disease-free survival) among the various clusters. The immune checkpoint gene CXCL1 displayed highly significant statistical differences between subtype A (Cluster 1) and subtype B (Cluster 2) in cervical cancer (CESC) samples. The gene set enrichment analysis identified the negative regulation of peptidase activity and the IL-17 signaling pathway, which link to subtype-specific differentially expressed genes (DEGs).ConclusionStatistical analysis of the various subtypes of CESC samples highlighted the importance of subtype-specific therapeutic targets. Additionally, it seeks to enhance the accuracy of prognostic predictions, thereby establishing a foundation for the formulation of personalized treatment approaches.https://www.frontiersin.org/articles/10.3389/fonc.2025.1574911/fullcervical cancerdisulfidptosisimmune cell infiltrationrisk signatureprognostic model |
| spellingShingle | Yadan Yao Xiaomin Yang Yuanxin Fu Yinmin Zhang Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis Frontiers in Oncology cervical cancer disulfidptosis immune cell infiltration risk signature prognostic model |
| title | Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis |
| title_full | Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis |
| title_fullStr | Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis |
| title_full_unstemmed | Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis |
| title_short | Immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis |
| title_sort | immunological features of various molecular subtypes of cervical cancer and their prognostic implications in the context of disulfidptosis |
| topic | cervical cancer disulfidptosis immune cell infiltration risk signature prognostic model |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2025.1574911/full |
| work_keys_str_mv | AT yadanyao immunologicalfeaturesofvariousmolecularsubtypesofcervicalcancerandtheirprognosticimplicationsinthecontextofdisulfidptosis AT xiaominyang immunologicalfeaturesofvariousmolecularsubtypesofcervicalcancerandtheirprognosticimplicationsinthecontextofdisulfidptosis AT yuanxinfu immunologicalfeaturesofvariousmolecularsubtypesofcervicalcancerandtheirprognosticimplicationsinthecontextofdisulfidptosis AT yinminzhang immunologicalfeaturesofvariousmolecularsubtypesofcervicalcancerandtheirprognosticimplicationsinthecontextofdisulfidptosis |