Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress
Drought stress has a significant impact on agricultural productivity, affecting key crops such as soybeans, the second most widely cultivated crop in the United States. Endophytic and rhizospheric microbial diversity analyses were conducted with soybean plants cultivated during the 2023 growing seas...
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MDPI AG
2024-12-01
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| author | Sandeep Gouli Aqsa Majeed Jinbao Liu David Moseley M. Shahid Mukhtar Jong Hyun Ham |
| author_facet | Sandeep Gouli Aqsa Majeed Jinbao Liu David Moseley M. Shahid Mukhtar Jong Hyun Ham |
| author_sort | Sandeep Gouli |
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| description | Drought stress has a significant impact on agricultural productivity, affecting key crops such as soybeans, the second most widely cultivated crop in the United States. Endophytic and rhizospheric microbial diversity analyses were conducted with soybean plants cultivated during the 2023 growing season amid extreme weather conditions of prolonged high temperatures and drought in Louisiana. Specifically, surviving and non-surviving soybean plants were collected from two plots of a Louisiana soybean field severely damaged by extreme heat and drought conditions in 2023. Although no significant difference was observed between surviving and non-surviving plants in microbial diversity of the rhizosphere, obvious differences were found in the structure of the endophytic microbial community in root tissues between the two plant conditions. In particular, the bacterial genera belonging to Proteobacteria, <i>Pseudomonas</i> and <i>Pantoea</i>, were predominant in the surviving root tissues, while the bacterial genus <i>Streptomyces</i> was conspicuously dominant in the non-surviving (dead) root tissues. Co-occurrence patterns and network centrality analyses enabled us to discern the intricate characteristics of operational taxonomic units (OTUs) within endophytic and rhizospheric networks. Additionally, we isolated and identified bacterial strains that enhanced soybean tolerance to drought stresses, which were sourced from soybean plants under a drought field condition. The 16S rDNA sequence analysis revealed that the beneficial bacterial strains belong to the genera <i>Acinetobacter</i>, <i>Pseudomonas</i>, <i>Enterobacter</i>, and <i>Stenotrophomonas</i>. Specific bacterial strains, particularly those identified as <i>Acinetobacter pittii</i> and <i>Pseudomonas</i> sp., significantly enhanced plant growth metrics and reduced drought stress indices in soybean plants through seed treatment. Overall, this study advances our understanding of the soybean-associated microbiome structure under drought stress, paving the way for future research to develop innovative strategies and biological tools for enhancing soybean resilience to drought. |
| format | Article |
| id | doaj-art-60d0babcc3514b648e19cea366dd7527 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Microorganisms |
| spelling | doaj-art-60d0babcc3514b648e19cea366dd75272025-08-20T02:43:46ZengMDPI AGMicroorganisms2076-26072024-12-011212263010.3390/microorganisms12122630Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought StressSandeep Gouli0Aqsa Majeed1Jinbao Liu2David Moseley3M. Shahid Mukhtar4Jong Hyun Ham5Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USADepartment of Biology, University of Alabama at Birmingham, 3100 Science & Engineering Complex–East Science Hall, 902 14 Street South, Birmingham, AL 35294, USADepartment of Biology, University of Alabama at Birmingham, 3100 Science & Engineering Complex–East Science Hall, 902 14 Street South, Birmingham, AL 35294, USADean Lee Research & Extension Center, Louisiana State University Agricultural Center, Alexandria, LA 71302, USADepartment of Biology, University of Alabama at Birmingham, 3100 Science & Engineering Complex–East Science Hall, 902 14 Street South, Birmingham, AL 35294, USADepartment of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USADrought stress has a significant impact on agricultural productivity, affecting key crops such as soybeans, the second most widely cultivated crop in the United States. Endophytic and rhizospheric microbial diversity analyses were conducted with soybean plants cultivated during the 2023 growing season amid extreme weather conditions of prolonged high temperatures and drought in Louisiana. Specifically, surviving and non-surviving soybean plants were collected from two plots of a Louisiana soybean field severely damaged by extreme heat and drought conditions in 2023. Although no significant difference was observed between surviving and non-surviving plants in microbial diversity of the rhizosphere, obvious differences were found in the structure of the endophytic microbial community in root tissues between the two plant conditions. In particular, the bacterial genera belonging to Proteobacteria, <i>Pseudomonas</i> and <i>Pantoea</i>, were predominant in the surviving root tissues, while the bacterial genus <i>Streptomyces</i> was conspicuously dominant in the non-surviving (dead) root tissues. Co-occurrence patterns and network centrality analyses enabled us to discern the intricate characteristics of operational taxonomic units (OTUs) within endophytic and rhizospheric networks. Additionally, we isolated and identified bacterial strains that enhanced soybean tolerance to drought stresses, which were sourced from soybean plants under a drought field condition. The 16S rDNA sequence analysis revealed that the beneficial bacterial strains belong to the genera <i>Acinetobacter</i>, <i>Pseudomonas</i>, <i>Enterobacter</i>, and <i>Stenotrophomonas</i>. Specific bacterial strains, particularly those identified as <i>Acinetobacter pittii</i> and <i>Pseudomonas</i> sp., significantly enhanced plant growth metrics and reduced drought stress indices in soybean plants through seed treatment. Overall, this study advances our understanding of the soybean-associated microbiome structure under drought stress, paving the way for future research to develop innovative strategies and biological tools for enhancing soybean resilience to drought.https://www.mdpi.com/2076-2607/12/12/2630soybean rhizosphereroot endospheremicrobial community |
| spellingShingle | Sandeep Gouli Aqsa Majeed Jinbao Liu David Moseley M. Shahid Mukhtar Jong Hyun Ham Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress Microorganisms soybean rhizosphere root endosphere microbial community |
| title | Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress |
| title_full | Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress |
| title_fullStr | Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress |
| title_full_unstemmed | Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress |
| title_short | Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress |
| title_sort | microbiome structures and beneficial bacteria in soybean roots under field conditions of prolonged high temperatures and drought stress |
| topic | soybean rhizosphere root endosphere microbial community |
| url | https://www.mdpi.com/2076-2607/12/12/2630 |
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