Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum
A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : ...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/297285 |
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| author | Najmul Hasan Mathurot Chaiharn Sauleha Khan Hira Khalid Nawab Sher Farhan Ahmed Siddiqui Muhammad Zain Siddiqui |
| author_facet | Najmul Hasan Mathurot Chaiharn Sauleha Khan Hira Khalid Nawab Sher Farhan Ahmed Siddiqui Muhammad Zain Siddiqui |
| author_sort | Najmul Hasan |
| collection | DOAJ |
| description | A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μBondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method. |
| format | Article |
| id | doaj-art-60c916b44c984b17b05a4b1d04f2f139 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-60c916b44c984b17b05a4b1d04f2f1392025-02-03T05:44:43ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/297285297285Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human SerumNajmul Hasan0Mathurot Chaiharn1Sauleha Khan2Hira Khalid3Nawab Sher4Farhan Ahmed Siddiqui5Muhammad Zain Siddiqui6Department of Microbiology, Faculty of Science, University of Karachi, Karachi 75270, PakistanDivision of Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, ThailandDepartment of Chemistry, Faculty of Science, University of Karachi, Karachi 75270, PakistanDepartment of Chemistry, Government College University, Lahore 54000, PakistanDepartment of Chemistry, Faculty of Science, University of Karachi, Karachi 75270, PakistanFaculty of Pharmacy, Federal Urdu University of Science & Technology, Karachi 75300, PakistanDepartment of Chemistry, Faculty of Science, Federal Urdu University of Science & Technology, Karachi 75300, PakistanA reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μBondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method.http://dx.doi.org/10.1155/2013/297285 |
| spellingShingle | Najmul Hasan Mathurot Chaiharn Sauleha Khan Hira Khalid Nawab Sher Farhan Ahmed Siddiqui Muhammad Zain Siddiqui Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum Journal of Analytical Methods in Chemistry |
| title | Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum |
| title_full | Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum |
| title_fullStr | Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum |
| title_full_unstemmed | Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum |
| title_short | Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum |
| title_sort | dual wavelength rp hplc method for simultaneous determination of two antispasmodic drugs an application in pharmaceutical and human serum |
| url | http://dx.doi.org/10.1155/2013/297285 |
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