Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa
Abstract Phloem, an exceptional plant vascular tissue, facilitates the transport of photoassimilates, RNAs, and other signaling substances from the leaves to the roots throughout the plant. Among the specialized phloem cells are the conductive sieve elements (SEs), which are unique in that they rema...
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BMC
2025-04-01
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| Online Access: | https://doi.org/10.1186/s12870-025-06439-4 |
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| author | Karolina Kułak Anna Samelak-Czajka Malgorzata Marszalek-Zenczak Kornel M. Michalak Magdalena Trybus Julia Minicka Paulina Jackowiak Agnieszka Bagniewska-Zadworna |
| author_facet | Karolina Kułak Anna Samelak-Czajka Malgorzata Marszalek-Zenczak Kornel M. Michalak Magdalena Trybus Julia Minicka Paulina Jackowiak Agnieszka Bagniewska-Zadworna |
| author_sort | Karolina Kułak |
| collection | DOAJ |
| description | Abstract Phloem, an exceptional plant vascular tissue, facilitates the transport of photoassimilates, RNAs, and other signaling substances from the leaves to the roots throughout the plant. Among the specialized phloem cells are the conductive sieve elements (SEs), which are unique in that they remain alive despite lacking several cell organelles, including the nucleus, plastids, and most mitochondria. These SEs contain a specific proteinaceous structure composed of phloem-specific proteins (P-proteins), whose function is not yet fully understood. Various P-proteins have been characterized in broad range of model species, including Arabidopsis thaliana, and reported in Fabaceae and Cucurbitaceae plants. To date, only one P-protein has been identified in the model tree species Populus trichocarpa. Given the presence of multiple P-protein encoding genes across numerous plant species, we hypothesized the existence of multiple such genes in the Populus genome. Our genomic analysis uncovered 12 genes being potential orthologues to one of A. thaliana P-protein – SEOR (sieve element occlusion-related) genes, which may contribute to the proteinaceous structures observed in differentiating sieve elements. Our transcriptomic and proteomic analyses confirmed the expression of at least seven of these genes, indicating that the protein structure visible in mature sieve elements in P. trichocarpa may be heterogeneous. |
| format | Article |
| id | doaj-art-60bd715cb0e14b028ebfcb1c6e12927b |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-60bd715cb0e14b028ebfcb1c6e12927b2025-08-20T02:17:13ZengBMCBMC Plant Biology1471-22292025-04-0125111410.1186/s12870-025-06439-4Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpaKarolina Kułak0Anna Samelak-Czajka1Malgorzata Marszalek-Zenczak2Kornel M. Michalak3Magdalena Trybus4Julia Minicka5Paulina Jackowiak6Agnieszka Bagniewska-Zadworna7Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz UniversityLaboratory of Single Cell Analyses, Institute of Bioorganic Chemistry, Polish Academy of SciencesLaboratory of Single Cell Analyses, Institute of Bioorganic Chemistry, Polish Academy of SciencesDepartment of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz UniversityLaboratory of Single Cell Analyses, Institute of Bioorganic Chemistry, Polish Academy of SciencesDepartment of Virology and Bacteriology, Institute of Plant Protection – National Research InstituteLaboratory of Single Cell Analyses, Institute of Bioorganic Chemistry, Polish Academy of SciencesDepartment of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz UniversityAbstract Phloem, an exceptional plant vascular tissue, facilitates the transport of photoassimilates, RNAs, and other signaling substances from the leaves to the roots throughout the plant. Among the specialized phloem cells are the conductive sieve elements (SEs), which are unique in that they remain alive despite lacking several cell organelles, including the nucleus, plastids, and most mitochondria. These SEs contain a specific proteinaceous structure composed of phloem-specific proteins (P-proteins), whose function is not yet fully understood. Various P-proteins have been characterized in broad range of model species, including Arabidopsis thaliana, and reported in Fabaceae and Cucurbitaceae plants. To date, only one P-protein has been identified in the model tree species Populus trichocarpa. Given the presence of multiple P-protein encoding genes across numerous plant species, we hypothesized the existence of multiple such genes in the Populus genome. Our genomic analysis uncovered 12 genes being potential orthologues to one of A. thaliana P-protein – SEOR (sieve element occlusion-related) genes, which may contribute to the proteinaceous structures observed in differentiating sieve elements. Our transcriptomic and proteomic analyses confirmed the expression of at least seven of these genes, indicating that the protein structure visible in mature sieve elements in P. trichocarpa may be heterogeneous.https://doi.org/10.1186/s12870-025-06439-4PhloemSieve elementP-proteinSEOSEORPopulus trichocarpa |
| spellingShingle | Karolina Kułak Anna Samelak-Czajka Malgorzata Marszalek-Zenczak Kornel M. Michalak Magdalena Trybus Julia Minicka Paulina Jackowiak Agnieszka Bagniewska-Zadworna Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa BMC Plant Biology Phloem Sieve element P-protein SEO SEOR Populus trichocarpa |
| title | Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa |
| title_full | Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa |
| title_fullStr | Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa |
| title_full_unstemmed | Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa |
| title_short | Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa |
| title_sort | identification of phloem specific proteinaseous structure heterogeneity in sieve element of populus trichocarpa |
| topic | Phloem Sieve element P-protein SEO SEOR Populus trichocarpa |
| url | https://doi.org/10.1186/s12870-025-06439-4 |
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