RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4
Abstract Background Circular RNAs (circRNAs) which are shown as a class of RNAs exhibit the importance in the regulation of gene expression and the development of biological process. However, the expression profile and molecular mechanism of circRNA ATXN7 (circATXN7) is still mostly uncertain in gas...
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BMC
2020-01-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-020-1106-5 |
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| author | Zhen Zhang Honglei Wu Zhaosheng Chen Guangchun Li Bin Liu |
| author_facet | Zhen Zhang Honglei Wu Zhaosheng Chen Guangchun Li Bin Liu |
| author_sort | Zhen Zhang |
| collection | DOAJ |
| description | Abstract Background Circular RNAs (circRNAs) which are shown as a class of RNAs exhibit the importance in the regulation of gene expression and the development of biological process. However, the expression profile and molecular mechanism of circRNA ATXN7 (circATXN7) is still mostly uncertain in gastric cancer (GC). Methods qRT-PCR analysis was performed to detect the expression of circATXN7, miR-4319 and ENTPD4 in GC tissues and cells. CCK-8, colony formation, EdU, flow cytometry, TUNEL and transwell assays were conducted to assess the effect of circATXN7 or miR-4319 on cell proliferation, apoptosis and invasion. In vivo assays were utilized to further analyze the function of circATXN7 on the tumorigenesis and progression of GC. The interaction between miR-4319 and circATXN7 (or ENTPD4) was verified using luciferase reporter and RNA pull-down assays. Results The results showed an upregulated circATXN7 expression in GC tissues and cell lines. Besides, silenced circATXN7 hampered the proliferation and invasion as well as promoted the apoptosis in GC cells. Moreover, low expression of miR-4319 was found in GC. It was determined that circATXN7 acted as a sponge for miR-4319 and had a negative association with miR-4319. We also found that miR-4319 upregulation restrained GC cell proliferation and migration whereas enhanced apoptosis. Subsequently, ENTPD4, the target gene of miR-4319, was found overexpressed in GC. Additionally, it was negatively correlated with miR-4319 whereas positively associated with circATXN7. In vivo experiments, circATXN7 silence was confirmed to inhibit GC tumor growth. Conclusions CircATXN7 promoted GC development through sponging miR-4319 and regulating ENTPD4, which identified circATXN7 as a new biomarker in GC. |
| format | Article |
| id | doaj-art-60ad0e18a7ac41fc9f4905444e8a8d81 |
| institution | DOAJ |
| issn | 1475-2867 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj-art-60ad0e18a7ac41fc9f4905444e8a8d812025-08-20T03:01:39ZengBMCCancer Cell International1475-28672020-01-0120111310.1186/s12935-020-1106-5RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4Zhen Zhang0Honglei Wu1Zhaosheng Chen2Guangchun Li3Bin Liu4Department of Gastroenterology, The Second Hospital of Shandong UniversityDepartment of Gastroenterology, The Second Hospital of Shandong UniversityDepartment of Gastroenterology, The Second Hospital of Shandong UniversityDepartment of Gastroenterology, The Second Hospital of Shandong UniversityDepartment of Gastroenterology, The Second Hospital of Shandong UniversityAbstract Background Circular RNAs (circRNAs) which are shown as a class of RNAs exhibit the importance in the regulation of gene expression and the development of biological process. However, the expression profile and molecular mechanism of circRNA ATXN7 (circATXN7) is still mostly uncertain in gastric cancer (GC). Methods qRT-PCR analysis was performed to detect the expression of circATXN7, miR-4319 and ENTPD4 in GC tissues and cells. CCK-8, colony formation, EdU, flow cytometry, TUNEL and transwell assays were conducted to assess the effect of circATXN7 or miR-4319 on cell proliferation, apoptosis and invasion. In vivo assays were utilized to further analyze the function of circATXN7 on the tumorigenesis and progression of GC. The interaction between miR-4319 and circATXN7 (or ENTPD4) was verified using luciferase reporter and RNA pull-down assays. Results The results showed an upregulated circATXN7 expression in GC tissues and cell lines. Besides, silenced circATXN7 hampered the proliferation and invasion as well as promoted the apoptosis in GC cells. Moreover, low expression of miR-4319 was found in GC. It was determined that circATXN7 acted as a sponge for miR-4319 and had a negative association with miR-4319. We also found that miR-4319 upregulation restrained GC cell proliferation and migration whereas enhanced apoptosis. Subsequently, ENTPD4, the target gene of miR-4319, was found overexpressed in GC. Additionally, it was negatively correlated with miR-4319 whereas positively associated with circATXN7. In vivo experiments, circATXN7 silence was confirmed to inhibit GC tumor growth. Conclusions CircATXN7 promoted GC development through sponging miR-4319 and regulating ENTPD4, which identified circATXN7 as a new biomarker in GC.https://doi.org/10.1186/s12935-020-1106-5circATXN7miR-4319ENTPD4Gastric cancer |
| spellingShingle | Zhen Zhang Honglei Wu Zhaosheng Chen Guangchun Li Bin Liu RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4 Cancer Cell International circATXN7 miR-4319 ENTPD4 Gastric cancer |
| title | RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4 |
| title_full | RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4 |
| title_fullStr | RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4 |
| title_full_unstemmed | RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4 |
| title_short | RETRACTED ARTICLE: Circular RNA ATXN7 promotes the development of gastric cancer through sponging miR-4319 and regulating ENTPD4 |
| title_sort | retracted article circular rna atxn7 promotes the development of gastric cancer through sponging mir 4319 and regulating entpd4 |
| topic | circATXN7 miR-4319 ENTPD4 Gastric cancer |
| url | https://doi.org/10.1186/s12935-020-1106-5 |
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