Isoform characterization of m6A in single cells identifies its role in RNA surveillance

Isoform characterization of m6A in single cells identifies its role in RNA surveillance

Abstract The distribution of m6A across various RNA isoforms and its heterogeneity within single cells are still not well understood. Here, we develop m6A-isoSC-seq, which employs both Oxford Nanopore long-read and Illumina short-read sequencing on the same 10x Genomics single-cell cDNA library with...

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Main Authors: Zhijun Ren, Jialiang He, Xiang Huang, Yan Gao, Chuanchuan Wei, Zehong Wu, Wenbing Guo, Feng Wang, Qingquan Zhao, Xiang Sun, Jie Zhang, Nan Cao, Lan Lin, Jinkai Wang, Yixian Cun
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-60869-0
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