Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, Indonesia
BACKGROUND: Antimicrobial resistance (AMR) surveillance data may be collected either from clinical isolates or from isolates cultured from the commensal microflora of individuals in the absence of infection. Escherichia coli is such a commensal that is widely used as indicator. AIM: This study aimed...
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524002327 |
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| author | Titiek Sulistyowati Tri Pudy Asmarawati Carolien Ruesen Heiman Wertheim Juliëtte Severin |
| author_facet | Titiek Sulistyowati Tri Pudy Asmarawati Carolien Ruesen Heiman Wertheim Juliëtte Severin |
| author_sort | Titiek Sulistyowati |
| collection | DOAJ |
| description | BACKGROUND: Antimicrobial resistance (AMR) surveillance data may be collected either from clinical isolates or from isolates cultured from the commensal microflora of individuals in the absence of infection. Escherichia coli is such a commensal that is widely used as indicator. AIM: This study aimed to determine the prevalence of AMR among E. coli isolated from layer farmers, catfish farmers, and nonfarmers in the largest poultry production region in Indonesia. METHODS: Farmers and nonfarmers (i.e., public healthcare center visitors) in Blitar region, East Java, Indonesia, were approached for screening from November to December 2022. A screening consisted of a rectal swab, which was cultured on an Eosin-methylene blue (EMB) agar plate. E. coli were identified using Vitek2® (bioMérieux). Antimicrobial susceptibility testing was performed using Vitek2® and interpreted according to CLSI clinical breakpoints. RESULTS: In total, 488 individuals were screened: 121 layer farmers, 69 catfish farmers, and 298 nonfarmers. Total isolates that could be recovered were 472 E. coli. E coli from farmers and nonfarmers showed remarkably high resistance to ampicillin and trimethoprim-sulfamethoxazole (Table). The third highest was ciprofloxacin resistance: from layer farmers 9.4%, and nonfarmers 8.6%, but from catfish farmers resistance was lower (2.5%). ESBL-producing E. coli from layer farmers was 2.6%, catfish farmers 5.0%, and nonfarmers 5.1%. Carbapenem resistance was not found. CONCLUSIONS: Overall, resistance rates were considerably high for ampicillin, cotrimoxazole, and the fluoroquinolones, and mostly similar among farmers and nonfarmers. Continued monitoring of local and national resistance trends will be important, to follow trends over time. |
| format | Article |
| id | doaj-art-60a263d61e0a4c10b07e6254eacb5cc8 |
| institution | DOAJ |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-60a263d61e0a4c10b07e6254eacb5cc82025-08-20T02:50:47ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-01391810.1016/j.jgar.2024.10.055Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, IndonesiaTitiek Sulistyowati0Tri Pudy Asmarawati1Carolien Ruesen2Heiman Wertheim3Juliëtte Severin4Public Health Laboratory, Balai Besar Laboratorium Kesehatan Masyarakat Surabaya, IndonesiaFaculty of Medicine, Airlangga University, Universitas Airlangga Hospital, IndonesiaCentre for Epidemiology and Surveillance of Infectious Diseases, National Institute for Public Health and the Environment, Bilthoven, the NetherlandsDepartment of Microbiology, Radboud UMC, Nijmegen, The NetherlandsMedical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, The NetherlandsBACKGROUND: Antimicrobial resistance (AMR) surveillance data may be collected either from clinical isolates or from isolates cultured from the commensal microflora of individuals in the absence of infection. Escherichia coli is such a commensal that is widely used as indicator. AIM: This study aimed to determine the prevalence of AMR among E. coli isolated from layer farmers, catfish farmers, and nonfarmers in the largest poultry production region in Indonesia. METHODS: Farmers and nonfarmers (i.e., public healthcare center visitors) in Blitar region, East Java, Indonesia, were approached for screening from November to December 2022. A screening consisted of a rectal swab, which was cultured on an Eosin-methylene blue (EMB) agar plate. E. coli were identified using Vitek2® (bioMérieux). Antimicrobial susceptibility testing was performed using Vitek2® and interpreted according to CLSI clinical breakpoints. RESULTS: In total, 488 individuals were screened: 121 layer farmers, 69 catfish farmers, and 298 nonfarmers. Total isolates that could be recovered were 472 E. coli. E coli from farmers and nonfarmers showed remarkably high resistance to ampicillin and trimethoprim-sulfamethoxazole (Table). The third highest was ciprofloxacin resistance: from layer farmers 9.4%, and nonfarmers 8.6%, but from catfish farmers resistance was lower (2.5%). ESBL-producing E. coli from layer farmers was 2.6%, catfish farmers 5.0%, and nonfarmers 5.1%. Carbapenem resistance was not found. CONCLUSIONS: Overall, resistance rates were considerably high for ampicillin, cotrimoxazole, and the fluoroquinolones, and mostly similar among farmers and nonfarmers. Continued monitoring of local and national resistance trends will be important, to follow trends over time.http://www.sciencedirect.com/science/article/pii/S2213716524002327Antimicrobial resistanceEscherichia colifarmersnonfarmers |
| spellingShingle | Titiek Sulistyowati Tri Pudy Asmarawati Carolien Ruesen Heiman Wertheim Juliëtte Severin Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, Indonesia Journal of Global Antimicrobial Resistance Antimicrobial resistance Escherichia coli farmers nonfarmers |
| title | Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, Indonesia |
| title_full | Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, Indonesia |
| title_fullStr | Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, Indonesia |
| title_full_unstemmed | Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, Indonesia |
| title_short | Antimicrobial resistance among commensal Escherichia coli isolated from layer farmers, catfish farmers, and nonfarmers in Blitar, East Java Province, Indonesia |
| title_sort | antimicrobial resistance among commensal escherichia coli isolated from layer farmers catfish farmers and nonfarmers in blitar east java province indonesia |
| topic | Antimicrobial resistance Escherichia coli farmers nonfarmers |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524002327 |
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