Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells
Background/Aim. Liver stellate cells play an important role in hepatic fibrosis, and its progression to cirrhosis. These cells show immunoreactivity with different monoclonal antibodies amongst which the commonest are α-smooth muscle actin (α-SMA) and glial fibrillary acid protein (GFAP). The aim of...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2006-01-01
|
| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2006/0042-84500606553T.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850253472745652224 |
|---|---|
| author | Tomanović Nada Boričić Ivan Brašanac Dimitrije |
| author_facet | Tomanović Nada Boričić Ivan Brašanac Dimitrije |
| author_sort | Tomanović Nada |
| collection | DOAJ |
| description | Background/Aim. Liver stellate cells play an important role in hepatic fibrosis, and its progression to cirrhosis. These cells show immunoreactivity with different monoclonal antibodies amongst which the commonest are α-smooth muscle actin (α-SMA) and glial fibrillary acid protein (GFAP). The aim of this study was to analyze stellate cell immunoreactivity for α-SMA and GFAP in tissue sections showing the signs of chronic viral B-hepatitis and compare it with those without histopathological changes. Methods. We included 12 tissue samples showing chronic viral B hepatitis in the different stages of fibrosis and 7 tissue samples showing no histopathological changes. Immunohistochemical staining was performed using the streptavidinbiotin method. Results. There was a regular presence of α-SMA immunoreactivity in tissue sections without histopathological changes in the portal tracts and also in liver parenchyma, while GFAP expression was noted in the periportal cavity. Tissue sections with the signs of chronic viral B hepatitis displayed very strong α-SMA expression in the portal tracts. A statistical analysis showed a positive correlation between the degree of liver fibrosis and α-SMA expression along the fibrous septa, whereas a negative correlation between the degree of liver fibrosis and α-SMA expression was present in the portal zone. Conclusion. This study showed the existance of two different stellate cell subpopulations in liver tissue. Differentiation between them was possible on the basis of SMA/GFAP expression. |
| format | Article |
| id | doaj-art-609ff464faed45f7bc9c5874ce71bee3 |
| institution | OA Journals |
| issn | 0042-8450 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-609ff464faed45f7bc9c5874ce71bee32025-08-20T01:57:24ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502006-01-0163655355710.2298/VSP0606553TImmunohistochemical analysis of α-SMA and GFAP expression in liver stellate cellsTomanović NadaBoričić IvanBrašanac DimitrijeBackground/Aim. Liver stellate cells play an important role in hepatic fibrosis, and its progression to cirrhosis. These cells show immunoreactivity with different monoclonal antibodies amongst which the commonest are α-smooth muscle actin (α-SMA) and glial fibrillary acid protein (GFAP). The aim of this study was to analyze stellate cell immunoreactivity for α-SMA and GFAP in tissue sections showing the signs of chronic viral B-hepatitis and compare it with those without histopathological changes. Methods. We included 12 tissue samples showing chronic viral B hepatitis in the different stages of fibrosis and 7 tissue samples showing no histopathological changes. Immunohistochemical staining was performed using the streptavidinbiotin method. Results. There was a regular presence of α-SMA immunoreactivity in tissue sections without histopathological changes in the portal tracts and also in liver parenchyma, while GFAP expression was noted in the periportal cavity. Tissue sections with the signs of chronic viral B hepatitis displayed very strong α-SMA expression in the portal tracts. A statistical analysis showed a positive correlation between the degree of liver fibrosis and α-SMA expression along the fibrous septa, whereas a negative correlation between the degree of liver fibrosis and α-SMA expression was present in the portal zone. Conclusion. This study showed the existance of two different stellate cell subpopulations in liver tissue. Differentiation between them was possible on the basis of SMA/GFAP expression.http://www.doiserbia.nb.rs/img/doi/0042-8450/2006/0042-84500606553T.pdfhepatitis Bimmunohistochemistrybiological markersbiopsyactinsglial fibrillary acidic protein |
| spellingShingle | Tomanović Nada Boričić Ivan Brašanac Dimitrije Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells Vojnosanitetski Pregled hepatitis B immunohistochemistry biological markers biopsy actins glial fibrillary acidic protein |
| title | Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells |
| title_full | Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells |
| title_fullStr | Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells |
| title_full_unstemmed | Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells |
| title_short | Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells |
| title_sort | immunohistochemical analysis of α sma and gfap expression in liver stellate cells |
| topic | hepatitis B immunohistochemistry biological markers biopsy actins glial fibrillary acidic protein |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2006/0042-84500606553T.pdf |
| work_keys_str_mv | AT tomanovicnada immunohistochemicalanalysisofasmaandgfapexpressioninliverstellatecells AT boricicivan immunohistochemicalanalysisofasmaandgfapexpressioninliverstellatecells AT brasanacdimitrije immunohistochemicalanalysisofasmaandgfapexpressioninliverstellatecells |