Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureus
Three Staphylococcus aureus isolates were used in the present study for extraction and quantitation of carotenoid pigment production. Different culture conditions were used to determine optimum pigmentation such as type of culture media, different pH values, temperature, and finally daily bacterial...
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| Format: | Article |
| Language: | English |
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Tikrit University
2019-12-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/451 |
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| author | Ghada A. Mohammad Suhair Taha Daod |
| author_facet | Ghada A. Mohammad Suhair Taha Daod |
| author_sort | Ghada A. Mohammad |
| collection | DOAJ |
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Three Staphylococcus aureus isolates were used in the present study for extraction and quantitation of carotenoid pigment production. Different culture conditions were used to determine optimum pigmentation such as type of culture media, different pH values, temperature, and finally daily bacterial sub-culturing for more than 2 weeks. Nutrient agar was found to be the best medium with the highest production (1.09). Optimum pH value was 6.5 and gave (1.8) carotenoid. Also results showed that pigment was produced at 37o C more than at 42oC. Daily repeated sub culturing had a negative impact on pigment production. Colonies gradualy lost pigmentation and became white in color. Also, sub-culturing was shown to affect the volume of the colonies as they became smaller on solid media.
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| format | Article |
| id | doaj-art-609dc2afc99544d399775c3d4d4b2c22 |
| institution | OA Journals |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-609dc2afc99544d399775c3d4d4b2c222025-08-20T02:37:09ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262019-12-0124710.25130/tjps.v24i7.451Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureusGhada A. MohammadSuhair Taha Daod Three Staphylococcus aureus isolates were used in the present study for extraction and quantitation of carotenoid pigment production. Different culture conditions were used to determine optimum pigmentation such as type of culture media, different pH values, temperature, and finally daily bacterial sub-culturing for more than 2 weeks. Nutrient agar was found to be the best medium with the highest production (1.09). Optimum pH value was 6.5 and gave (1.8) carotenoid. Also results showed that pigment was produced at 37o C more than at 42oC. Daily repeated sub culturing had a negative impact on pigment production. Colonies gradualy lost pigmentation and became white in color. Also, sub-culturing was shown to affect the volume of the colonies as they became smaller on solid media. https://tjpsj.org/index.php/tjps/article/view/451S. aureusCarotenoidphysiological factorsrepeated subculture |
| spellingShingle | Ghada A. Mohammad Suhair Taha Daod Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureus Tikrit Journal of Pure Science S. aureus Carotenoid physiological factors repeated subculture |
| title | Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureus |
| title_full | Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureus |
| title_fullStr | Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureus |
| title_full_unstemmed | Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureus |
| title_short | Detection the role of physiological factors to produce carotenoid pigment in Staphylococcus aureus |
| title_sort | detection the role of physiological factors to produce carotenoid pigment in staphylococcus aureus |
| topic | S. aureus Carotenoid physiological factors repeated subculture |
| url | https://tjpsj.org/index.php/tjps/article/view/451 |
| work_keys_str_mv | AT ghadaamohammad detectiontheroleofphysiologicalfactorstoproducecarotenoidpigmentinstaphylococcusaureus AT suhairtahadaod detectiontheroleofphysiologicalfactorstoproducecarotenoidpigmentinstaphylococcusaureus |