Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay
The oxidative burst of human blood cells in the presence of different metal materials was investigated using chemiluminescence assay. Commercial pure titanium (Ti), titanium alloy (Ti6Al4V), and stainless steel 316L (SS) in particulate form with <20 μm in size were used. The effect of particulate...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2012/380845 |
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| _version_ | 1850120675667214336 |
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| author | Sandra C. P. Cachinho Fanrong Pu |
| author_facet | Sandra C. P. Cachinho Fanrong Pu |
| author_sort | Sandra C. P. Cachinho |
| collection | DOAJ |
| description | The oxidative burst of human blood cells in the presence of different metal materials was investigated using chemiluminescence assay. Commercial pure titanium (Ti), titanium alloy (Ti6Al4V), and stainless steel 316L (SS) in particulate form with <20 μm in size were used. The effect of particulate materials opsonisation on the upregulation of the respiratory burst production by blood cells was also assessed. The largest chemiluminescence response was achieved after simultaneous injection of the stimulants fMLP+PMA. Moreover, Ti and SS induced a greater inflammatory reaction compared to Ti6Al4V, since the respiratory burst mounted was higher for both materials after opsonisation treatment. These results suggest that in vitro chemiluminescence response and respiratory burst measurements proved to be composition and treatment dependent. |
| format | Article |
| id | doaj-art-609ae2b098814b2fa1aa8745c5624c2d |
| institution | OA Journals |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-609ae2b098814b2fa1aa8745c5624c2d2025-08-20T02:35:18ZengWileyInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/380845380845Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin AssaySandra C. P. Cachinho0Fanrong Pu1Clinical Engineering, University of Liverpool, Liverpool L69 3GA, UKClinical Engineering, University of Liverpool, Liverpool L69 3GA, UKThe oxidative burst of human blood cells in the presence of different metal materials was investigated using chemiluminescence assay. Commercial pure titanium (Ti), titanium alloy (Ti6Al4V), and stainless steel 316L (SS) in particulate form with <20 μm in size were used. The effect of particulate materials opsonisation on the upregulation of the respiratory burst production by blood cells was also assessed. The largest chemiluminescence response was achieved after simultaneous injection of the stimulants fMLP+PMA. Moreover, Ti and SS induced a greater inflammatory reaction compared to Ti6Al4V, since the respiratory burst mounted was higher for both materials after opsonisation treatment. These results suggest that in vitro chemiluminescence response and respiratory burst measurements proved to be composition and treatment dependent.http://dx.doi.org/10.1155/2012/380845 |
| spellingShingle | Sandra C. P. Cachinho Fanrong Pu Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay International Journal of Biomaterials |
| title | Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay |
| title_full | Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay |
| title_fullStr | Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay |
| title_full_unstemmed | Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay |
| title_short | Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay |
| title_sort | free radical production in immune cell systems induced by ti ti6al4v and ss assessed by chemiluminescence probe pholasin assay |
| url | http://dx.doi.org/10.1155/2012/380845 |
| work_keys_str_mv | AT sandracpcachinho freeradicalproductioninimmunecellsystemsinducedbytiti6al4vandssassessedbychemiluminescenceprobepholasinassay AT fanrongpu freeradicalproductioninimmunecellsystemsinducedbytiti6al4vandssassessedbychemiluminescenceprobepholasinassay |