Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2
Aniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain desig...
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2024-11-01
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| author | Zetian Luo Jiajun Ma Lei Huang Dahui Li Guohui Gao Yihe Zhao Agostinho Antunes Meitong Li |
| author_facet | Zetian Luo Jiajun Ma Lei Huang Dahui Li Guohui Gao Yihe Zhao Agostinho Antunes Meitong Li |
| author_sort | Zetian Luo |
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| description | Aniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain designated DH-2 was isolated from soil contaminated with aniline. The strain was identified as belonging to the genus <i>Rhodococcus</i>. The optimal conditions for the growth and aniline degradation by strain DH-2 were determined to be pH 8.0 and 35 °C, respectively. Under these conditions, the degradation rate of aniline at a concentration of 1000 mg/L exceeded 90% within 36 h. Even in the presence of 4% NaCl, the degradation rate remained above 60%. HPLC–MS analysis revealed that the aniline degradation pathway of strain DH-2 follows the catechol pathway. Additionally, strain DH-2 is capable of utilizing crude oil as the sole carbon source, achieving a degradation rate of 91.0% for 2% crude oil concentration within 4 days. In soil modeling experiments, strain DH-2 was observed to degrade aniline and crude oil under triple stress conditions, including 1000 mg/L aniline, 2% crude oil, and 20 mg/L Fe(II) or Pb(II). Complete degradation of aniline and crude oil was achieved after 3 days and 12 days, respectively. The addition of Fe(II) or Pb(II) ions was found to enhance the degradation ability of DH-2. These results demonstrate that strain DH-2 is an extremely effective biodegradable strain, with potential applications in the remediation of environments contaminated with aniline and crude oil, even in the presence of heavy metals. |
| format | Article |
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| institution | Kabale University |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-608fb65855a441e5bb6f68065d5e015f2024-11-26T18:14:50ZengMDPI AGMicroorganisms2076-26072024-11-011211229310.3390/microorganisms12112293Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2Zetian Luo0Jiajun Ma1Lei Huang2Dahui Li3Guohui Gao4Yihe Zhao5Agostinho Antunes6Meitong Li7Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, ChinaTianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, ChinaTianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, ChinaTianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, ChinaTianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, ChinaCIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Porto, PortugalCIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208 Porto, PortugalTianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, ChinaAniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain designated DH-2 was isolated from soil contaminated with aniline. The strain was identified as belonging to the genus <i>Rhodococcus</i>. The optimal conditions for the growth and aniline degradation by strain DH-2 were determined to be pH 8.0 and 35 °C, respectively. Under these conditions, the degradation rate of aniline at a concentration of 1000 mg/L exceeded 90% within 36 h. Even in the presence of 4% NaCl, the degradation rate remained above 60%. HPLC–MS analysis revealed that the aniline degradation pathway of strain DH-2 follows the catechol pathway. Additionally, strain DH-2 is capable of utilizing crude oil as the sole carbon source, achieving a degradation rate of 91.0% for 2% crude oil concentration within 4 days. In soil modeling experiments, strain DH-2 was observed to degrade aniline and crude oil under triple stress conditions, including 1000 mg/L aniline, 2% crude oil, and 20 mg/L Fe(II) or Pb(II). Complete degradation of aniline and crude oil was achieved after 3 days and 12 days, respectively. The addition of Fe(II) or Pb(II) ions was found to enhance the degradation ability of DH-2. These results demonstrate that strain DH-2 is an extremely effective biodegradable strain, with potential applications in the remediation of environments contaminated with aniline and crude oil, even in the presence of heavy metals.https://www.mdpi.com/2076-2607/12/11/2293aniline degradation<i>Rhodococcus</i> sp.catechol pathwaycrude oilcombined pollution |
| spellingShingle | Zetian Luo Jiajun Ma Lei Huang Dahui Li Guohui Gao Yihe Zhao Agostinho Antunes Meitong Li Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2 Microorganisms aniline degradation <i>Rhodococcus</i> sp. catechol pathway crude oil combined pollution |
| title | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2 |
| title_full | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2 |
| title_fullStr | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2 |
| title_full_unstemmed | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2 |
| title_short | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain <i>Rhodococcus</i> sp. DH-2 |
| title_sort | biodegradation of crude oil and aniline by heavy metal tolerant strain i rhodococcus i sp dh 2 |
| topic | aniline degradation <i>Rhodococcus</i> sp. catechol pathway crude oil combined pollution |
| url | https://www.mdpi.com/2076-2607/12/11/2293 |
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