MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway
Jingjing Ni, Yan Ma, Jianping Qiu Department of Obstetrics and Gynecology, The Affiliated Suzhou Hospital of Nanjing Medical University (Suzhou Municipal Hospital), Suzhou, Jiangsu, 215000, People’s Republic of ChinaCorrespondence: Jianping Qiu, Email 15722703657@163.comObjective: This study aimed t...
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Dove Medical Press
2025-07-01
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| Series: | International Journal of Women's Health |
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| Online Access: | https://www.dovepress.com/mir-499a-5p-promotes-the-proliferation-and-migration-of-uterine-leiomy-peer-reviewed-fulltext-article-IJWH |
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| author | Ni J Ma Y Qiu J |
| author_facet | Ni J Ma Y Qiu J |
| author_sort | Ni J |
| collection | DOAJ |
| description | Jingjing Ni, Yan Ma, Jianping Qiu Department of Obstetrics and Gynecology, The Affiliated Suzhou Hospital of Nanjing Medical University (Suzhou Municipal Hospital), Suzhou, Jiangsu, 215000, People’s Republic of ChinaCorrespondence: Jianping Qiu, Email 15722703657@163.comObjective: This study aimed to investigate the functional role and underlying mechanism of miR-499a-5p in the progression of uterine leiomyoma (ULM).Methods: Expression levels of miR-499a-5p were analyzed in ULM tissues and cells. Functional assays were conducted to evaluate the effects of miR-499a-5p knockdown or overexpression on cellular proliferation, migration, apoptosis, and cell cycle progression. The involvement of the Wnt/β-catenin signaling pathway was assessed using pathway-specific protein markers and lithium chloride (LiCl) as a chemical activator.Results: miR-499a-5p was significantly upregulated in ULM tissues and cells. Its knockdown inhibited proliferation and migration, induced apoptosis, and caused cell cycle arrest at the G0/G1 phase. Additionally, downregulation of miR-499a-5p suppressed the activation of the Wnt/β-catenin pathway, an effect that was reversed by LiCl treatment.Conclusion: miR-499a-5p facilitates the development of uterine leiomyoma by activating the Wnt/β-catenin signaling pathway. These findings suggest that miR-499a-5p may serve as a promising molecular biomarker and a potential therapeutic target for ULM management.Keywords: miR-499a-5p, uterine leiomyoma, Wnt/β-catenin signaling pathway, proliferation, migration |
| format | Article |
| id | doaj-art-608d9da7b16c4acf8d27f272bd41231d |
| institution | Kabale University |
| issn | 1179-1411 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Women's Health |
| spelling | doaj-art-608d9da7b16c4acf8d27f272bd41231d2025-08-20T03:31:21ZengDove Medical PressInternational Journal of Women's Health1179-14112025-07-01Volume 17Issue 122452253105005MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling PathwayNi J0Ma Y1Qiu J2Department of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyJingjing Ni, Yan Ma, Jianping Qiu Department of Obstetrics and Gynecology, The Affiliated Suzhou Hospital of Nanjing Medical University (Suzhou Municipal Hospital), Suzhou, Jiangsu, 215000, People’s Republic of ChinaCorrespondence: Jianping Qiu, Email 15722703657@163.comObjective: This study aimed to investigate the functional role and underlying mechanism of miR-499a-5p in the progression of uterine leiomyoma (ULM).Methods: Expression levels of miR-499a-5p were analyzed in ULM tissues and cells. Functional assays were conducted to evaluate the effects of miR-499a-5p knockdown or overexpression on cellular proliferation, migration, apoptosis, and cell cycle progression. The involvement of the Wnt/β-catenin signaling pathway was assessed using pathway-specific protein markers and lithium chloride (LiCl) as a chemical activator.Results: miR-499a-5p was significantly upregulated in ULM tissues and cells. Its knockdown inhibited proliferation and migration, induced apoptosis, and caused cell cycle arrest at the G0/G1 phase. Additionally, downregulation of miR-499a-5p suppressed the activation of the Wnt/β-catenin pathway, an effect that was reversed by LiCl treatment.Conclusion: miR-499a-5p facilitates the development of uterine leiomyoma by activating the Wnt/β-catenin signaling pathway. These findings suggest that miR-499a-5p may serve as a promising molecular biomarker and a potential therapeutic target for ULM management.Keywords: miR-499a-5p, uterine leiomyoma, Wnt/β-catenin signaling pathway, proliferation, migrationhttps://www.dovepress.com/mir-499a-5p-promotes-the-proliferation-and-migration-of-uterine-leiomy-peer-reviewed-fulltext-article-IJWHmiR-499a-5puterine leiomyomaWnt/β-catenin signaling pathwayproliferationmigration |
| spellingShingle | Ni J Ma Y Qiu J MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway International Journal of Women's Health miR-499a-5p uterine leiomyoma Wnt/β-catenin signaling pathway proliferation migration |
| title | MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway |
| title_full | MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway |
| title_fullStr | MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway |
| title_full_unstemmed | MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway |
| title_short | MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway |
| title_sort | mir 499a 5p promotes the proliferation and migration of uterine leiomyoma cells by activating the wnt amp beta catenin signaling pathway |
| topic | miR-499a-5p uterine leiomyoma Wnt/β-catenin signaling pathway proliferation migration |
| url | https://www.dovepress.com/mir-499a-5p-promotes-the-proliferation-and-migration-of-uterine-leiomy-peer-reviewed-fulltext-article-IJWH |
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