Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells
Background: Pluripotent stem cells (PSCs) are capable of differencing into various cell types in the body, providing them valuable for therapy of degenerative diseases. Patient-specific treatments using PSCs, such as mesenchymal stem cells in patient’s own body, may reduce the risk of immune rejecti...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2024-12-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2714&vmd=Full |
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| author | Yeon-Ji Lee In-Won Lee Tae-Suk Kim Bo-Gyeong Seo Sang-Ki Baek Cheol Hwangbo Joon-Hee Lee |
| author_facet | Yeon-Ji Lee In-Won Lee Tae-Suk Kim Bo-Gyeong Seo Sang-Ki Baek Cheol Hwangbo Joon-Hee Lee |
| author_sort | Yeon-Ji Lee |
| collection | DOAJ |
| description | Background: Pluripotent stem cells (PSCs) are capable of differencing into various cell types in the body, providing them valuable for therapy of degenerative diseases. Patient-specific treatments using PSCs, such as mesenchymal stem cells in patient’s own body, may reduce the risk of immune rejection. Inducing the differentiation of PSCs into vascular endothelial cells (ECs) altering culture conditions or using specific growth factors is able to applied to the treatment of vascular diseases. The purpose of this study was to induce the differentiation of porcine epiblast stem cells (pEpiSCs), bone marrow-derived mesenchymal stem cells (pBM-MSCs) and adipose-derived mesenchymal stem cells (pA-MSCs) into ECs and then examine the functionality of vascular ECs.
Methods: Porcine pEpiSCs, pBM-MSCs and pA-MSCs were induced to differentiate into ECs on matrigel-coated plates in differentiation medium (EBM-2 + 50 ng/mL of VEGF) for 8 days. Cells differentiated from these stem cells were isolated using CD-31 positive (+) magnetic-activated cell sorting (MACS) and then proliferated in M199 medium. Evaluation of ECs differentiated from these stem cells was treated with capillary-like structure formation and three-dimensional spheroid sprouting assay.
Results: Porcine pEpiSCs, pBM-MSCs and pA-MSCs showed similar expression of pluripotency-related genes (OCT-3/4. NANOG, SOX2). These stem cells were differentiated into vascular ECs, but showed different morphologies after the differentiation. Cells differentiated from pEpiSCs showed an elongated spindle-like morphology, whereas cells differentiated from pBM-MSCs showed a round pebble-like morphology. In the case of pA-MSCs, these two morphologies were mixed with each other. Additionally, vascular ECs differentiated from these stem cells showed different formation of capillary-like structure formation and three-dimensional spheroid sprouting assay.
Conclusions: Cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs presented the functionality of different vascular ECs, demonstrating the potential of the excellent ECs differentiated from pEpiSCs. |
| format | Article |
| id | doaj-art-60814d36a5bd479599c6331562dab6a5 |
| institution | Kabale University |
| issn | 2671-4639 2671-4663 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj-art-60814d36a5bd479599c6331562dab6a52025-01-14T15:25:17ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632024-12-0139427829310.12750/JARB.39.4.278Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cellsYeon-Ji Lee0https://orcid.org/0009-0004-9669-2722In-Won Lee1https://orcid.org/0000-0002-1311-1469Tae-Suk Kim2https://orcid.org/0009-0007-0789-1968Bo-Gyeong Seo3https://orcid.org/0009-0006-3854-5622Sang-Ki Baek4https://orcid.org/0000-0001-9552-5050Cheol Hwangbo5https://orcid.org/0000-0001-6505-3080 Joon-Hee Lee6https://orcid.org/0000-0003-4921-0909Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, KoreaDepartment of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, KoreaDepartment of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, KoreaDivision of Life Science, College of Natural Sciences, Gyeongsang National University, Jinju 52828, KoreaGyeongsangnamdo Livestock Experiment Station, Sancheong 52263, KoreaDivision of Life Science, College of Natural Sciences, Gyeongsang National University, Jinju 52828, KoreaDepartment of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, KoreaBackground: Pluripotent stem cells (PSCs) are capable of differencing into various cell types in the body, providing them valuable for therapy of degenerative diseases. Patient-specific treatments using PSCs, such as mesenchymal stem cells in patient’s own body, may reduce the risk of immune rejection. Inducing the differentiation of PSCs into vascular endothelial cells (ECs) altering culture conditions or using specific growth factors is able to applied to the treatment of vascular diseases. The purpose of this study was to induce the differentiation of porcine epiblast stem cells (pEpiSCs), bone marrow-derived mesenchymal stem cells (pBM-MSCs) and adipose-derived mesenchymal stem cells (pA-MSCs) into ECs and then examine the functionality of vascular ECs. Methods: Porcine pEpiSCs, pBM-MSCs and pA-MSCs were induced to differentiate into ECs on matrigel-coated plates in differentiation medium (EBM-2 + 50 ng/mL of VEGF) for 8 days. Cells differentiated from these stem cells were isolated using CD-31 positive (+) magnetic-activated cell sorting (MACS) and then proliferated in M199 medium. Evaluation of ECs differentiated from these stem cells was treated with capillary-like structure formation and three-dimensional spheroid sprouting assay. Results: Porcine pEpiSCs, pBM-MSCs and pA-MSCs showed similar expression of pluripotency-related genes (OCT-3/4. NANOG, SOX2). These stem cells were differentiated into vascular ECs, but showed different morphologies after the differentiation. Cells differentiated from pEpiSCs showed an elongated spindle-like morphology, whereas cells differentiated from pBM-MSCs showed a round pebble-like morphology. In the case of pA-MSCs, these two morphologies were mixed with each other. Additionally, vascular ECs differentiated from these stem cells showed different formation of capillary-like structure formation and three-dimensional spheroid sprouting assay. Conclusions: Cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs presented the functionality of different vascular ECs, demonstrating the potential of the excellent ECs differentiated from pEpiSCs.https://www.e-jarb.org/journal/view.html?uid=2714&vmd=Fulladipose-derived mesenchymal stem cellsbone marrow-derived mesenchymal stem cellsendothelial cellsepiblast stem cellsin vitro differentiation |
| spellingShingle | Yeon-Ji Lee In-Won Lee Tae-Suk Kim Bo-Gyeong Seo Sang-Ki Baek Cheol Hwangbo Joon-Hee Lee Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells Journal of Animal Reproduction and Biotechnology adipose-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells endothelial cells epiblast stem cells in vitro differentiation |
| title | Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells |
| title_full | Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells |
| title_fullStr | Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells |
| title_full_unstemmed | Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells |
| title_short | Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells |
| title_sort | functionality of endothelial cells differentiated from porcine epiblast stem cells bone marrow derived mesenchymal stem cells and adipose derived mesenchymal stem cells |
| topic | adipose-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells endothelial cells epiblast stem cells in vitro differentiation |
| url | https://www.e-jarb.org/journal/view.html?uid=2714&vmd=Full |
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