Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon
Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon...
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The Journal of Infection in Developing Countries
2021-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/13558 |
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| author | Silvia Cristina da Silva Pedroso Karla Valéria Batista Lima Ismari Perini Furlaneto Yan Corrêa Rodrigues Darlene Kássia Saraiva Queiroz Pantoja Alex Junior Souza de Souza Washington Luiz Assunção Pereira |
| author_facet | Silvia Cristina da Silva Pedroso Karla Valéria Batista Lima Ismari Perini Furlaneto Yan Corrêa Rodrigues Darlene Kássia Saraiva Queiroz Pantoja Alex Junior Souza de Souza Washington Luiz Assunção Pereira |
| author_sort | Silvia Cristina da Silva Pedroso |
| collection | DOAJ |
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Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region.
Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively.
Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region.
Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil.
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| format | Article |
| id | doaj-art-6068d7f79cc64feb8f97a33dcdd05177 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-6068d7f79cc64feb8f97a33dcdd051772025-08-20T02:16:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-05-01150510.3855/jidc.13558Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian AmazonSilvia Cristina da Silva Pedroso 0Karla Valéria Batista Lima 1Ismari Perini Furlaneto2Yan Corrêa Rodrigues 3Darlene Kássia Saraiva Queiroz Pantoja 4Alex Junior Souza de Souza5Washington Luiz Assunção Pereira6Post-graduate Program in Animal Science, Federal University of Pará, Belém, BrazilBacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, BrazilPost-graduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, BrazilPost-graduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, BrazilPost-graduate Program in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, BrazilMax Planck University Center, Indaiatuba, BrazilInstitute of Animal Health and Production, Federal Rural University of Amazonia, Belém, Brazil Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region. Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively. Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region. Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil. https://jidc.org/index.php/journal/article/view/13558Granulomamycobacteriosisnon-TB mycobacteriasanitary inspectionzoonosis |
| spellingShingle | Silvia Cristina da Silva Pedroso Karla Valéria Batista Lima Ismari Perini Furlaneto Yan Corrêa Rodrigues Darlene Kássia Saraiva Queiroz Pantoja Alex Junior Souza de Souza Washington Luiz Assunção Pereira Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon Journal of Infection in Developing Countries Granuloma mycobacteriosis non-TB mycobacteria sanitary inspection zoonosis |
| title | Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon |
| title_full | Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon |
| title_fullStr | Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon |
| title_full_unstemmed | Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon |
| title_short | Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon |
| title_sort | bovine tuberculosis due to mycobacterium bovis and other mycobacteria among water buffalo bubalus bubalis from the brazilian amazon |
| topic | Granuloma mycobacteriosis non-TB mycobacteria sanitary inspection zoonosis |
| url | https://jidc.org/index.php/journal/article/view/13558 |
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