Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles
The interest on plants has been focalized due to their biological activities. Extracts or fractions from plants in biodegradable polymeric nanoparticles (NP) provide many advantages on application studies. The encapsulation of the extract or fraction in NP is determined for the establishment of the...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/9086467 |
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| author | Claudia Janeth Martínez-Rivas Rocío Álvarez-Román Catalina Rivas-Morales Abdelhamid Elaissari Hatem Fessi Sergio Arturo Galindo-Rodríguez |
| author_facet | Claudia Janeth Martínez-Rivas Rocío Álvarez-Román Catalina Rivas-Morales Abdelhamid Elaissari Hatem Fessi Sergio Arturo Galindo-Rodríguez |
| author_sort | Claudia Janeth Martínez-Rivas |
| collection | DOAJ |
| description | The interest on plants has been focalized due to their biological activities. Extracts or fractions from plants in biodegradable polymeric nanoparticles (NP) provide many advantages on application studies. The encapsulation of the extract or fraction in NP is determined for the establishment of the test dose. HPLC method is an alternative to calculate this parameter. An analytical method based on HPLC for quantification of a hexane fraction from L. frutescens was developed and validated according to ICH. Different concentrations of the hexane fraction from leaves (HFL) were prepared (100–600 μg/mL). Linearity, limit of detection, limit of quantification, and intra- and interday precision parameters were determined. HFL was encapsulated by nanoprecipitation technique and analyzed by HPLC for quantitative aspect. The method was linear and precise for the quantification of the HFL components. NP size was 190 nm with homogeneous size distribution. Through validation method, it was determined that the encapsulation of components (1), (2), (3), and (4) was 44, 74, 86, and 97%, respectively. A simple, repeatable, and reproducible methodology was developed for the propose of quantifying the components of a vegetable material loaded in NP, using as a model the hexane fraction of L. frutescens leaves. |
| format | Article |
| id | doaj-art-6064ee3ba33643d5875de08ecc80dbf1 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-6064ee3ba33643d5875de08ecc80dbf12025-08-20T03:34:48ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/90864679086467Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric NanoparticlesClaudia Janeth Martínez-Rivas0Rocío Álvarez-Román1Catalina Rivas-Morales2Abdelhamid Elaissari3Hatem Fessi4Sergio Arturo Galindo-Rodríguez5Facultad de Ciencias Biológicas, Laboratorio de Nanotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba s/n, 66455 San Nicolás de los Garza, NL, MexicoFacultad de Medicina, Departamento de Química Analítica, Universidad Autónoma de Nuevo León, Av. Fco. I. Madero y Dr. E. Aguirre Pequeño s/n, 64460 Monterrey, NL, MexicoFacultad de Ciencias Biológicas, Laboratorio de Nanotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba s/n, 66455 San Nicolás de los Garza, NL, MexicoUniv Lyon, Université Claude Bernard Lyon 1, CNRS, LAGEP-UMR 5007, 69622 Lyon, FranceUniv Lyon, Université Claude Bernard Lyon 1, CNRS, LAGEP-UMR 5007, 69622 Lyon, FranceFacultad de Ciencias Biológicas, Laboratorio de Nanotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba s/n, 66455 San Nicolás de los Garza, NL, MexicoThe interest on plants has been focalized due to their biological activities. Extracts or fractions from plants in biodegradable polymeric nanoparticles (NP) provide many advantages on application studies. The encapsulation of the extract or fraction in NP is determined for the establishment of the test dose. HPLC method is an alternative to calculate this parameter. An analytical method based on HPLC for quantification of a hexane fraction from L. frutescens was developed and validated according to ICH. Different concentrations of the hexane fraction from leaves (HFL) were prepared (100–600 μg/mL). Linearity, limit of detection, limit of quantification, and intra- and interday precision parameters were determined. HFL was encapsulated by nanoprecipitation technique and analyzed by HPLC for quantitative aspect. The method was linear and precise for the quantification of the HFL components. NP size was 190 nm with homogeneous size distribution. Through validation method, it was determined that the encapsulation of components (1), (2), (3), and (4) was 44, 74, 86, and 97%, respectively. A simple, repeatable, and reproducible methodology was developed for the propose of quantifying the components of a vegetable material loaded in NP, using as a model the hexane fraction of L. frutescens leaves.http://dx.doi.org/10.1155/2017/9086467 |
| spellingShingle | Claudia Janeth Martínez-Rivas Rocío Álvarez-Román Catalina Rivas-Morales Abdelhamid Elaissari Hatem Fessi Sergio Arturo Galindo-Rodríguez Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles Journal of Analytical Methods in Chemistry |
| title | Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles |
| title_full | Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles |
| title_fullStr | Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles |
| title_full_unstemmed | Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles |
| title_short | Quantitative Aspect of Leucophyllum frutescens Fraction before and after Encapsulation in Polymeric Nanoparticles |
| title_sort | quantitative aspect of leucophyllum frutescens fraction before and after encapsulation in polymeric nanoparticles |
| url | http://dx.doi.org/10.1155/2017/9086467 |
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