Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study.
Intense cancer research is underway to discover possible therapies but no major breakthrough appears to be in sight in terms of its cure when diagnosed late. The cytostatic and growth inhibitory effect of high pressure on cells is well documented. In the present study, two cancer cell lines viz. MDA...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0311685 |
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| author | Mohsin Ali Khan Zaw Ali Khan Ishrat Husain Shivbrat Upadhyay Sarina Zehra Rumana Ahmad |
| author_facet | Mohsin Ali Khan Zaw Ali Khan Ishrat Husain Shivbrat Upadhyay Sarina Zehra Rumana Ahmad |
| author_sort | Mohsin Ali Khan |
| collection | DOAJ |
| description | Intense cancer research is underway to discover possible therapies but no major breakthrough appears to be in sight in terms of its cure when diagnosed late. The cytostatic and growth inhibitory effect of high pressure on cells is well documented. In the present study, two cancer cell lines viz. MDA-MB-231 (breast carcinoma) and A549 (lung carcinoma) and one normal cell line (Vero) were subjected to increased pressure of 18 psi in a specially constructed pressure chamber. It was found that a pressure of 18 psi induced a significant change in the growth parameters of both cancer cell lines versus normal cells. Exposure to increased pressure greatly increased the proportion of MDA-MB-231 cells in the S phase while concurrently reducing the number of cells in the G0/G1 phase as compared to their untreated counterparts. SEM and AFM analysis revealed presence of characteristic 'pores' and 'pits' on the cell surface of pressure-treated versus untreated cancer cells. TEM analysis also revealed significant intracellular differences between pressure-treated and untreated cancer cells. Hyperbaric nitrogen therapy is proposed as a novel cancer-treatment modality involving administration of N2 at the tumor site in murine models of breast and lung cancer. This would eventually pave the way for development of a device effective treatment strategy for human tumors in future. |
| format | Article |
| id | doaj-art-604f49cc9dd243b1bdbe1cb691b98fba |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-604f49cc9dd243b1bdbe1cb691b98fba2025-08-20T01:51:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031168510.1371/journal.pone.0311685Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study.Mohsin Ali KhanZaw Ali KhanIshrat HusainShivbrat UpadhyaySarina ZehraRumana AhmadIntense cancer research is underway to discover possible therapies but no major breakthrough appears to be in sight in terms of its cure when diagnosed late. The cytostatic and growth inhibitory effect of high pressure on cells is well documented. In the present study, two cancer cell lines viz. MDA-MB-231 (breast carcinoma) and A549 (lung carcinoma) and one normal cell line (Vero) were subjected to increased pressure of 18 psi in a specially constructed pressure chamber. It was found that a pressure of 18 psi induced a significant change in the growth parameters of both cancer cell lines versus normal cells. Exposure to increased pressure greatly increased the proportion of MDA-MB-231 cells in the S phase while concurrently reducing the number of cells in the G0/G1 phase as compared to their untreated counterparts. SEM and AFM analysis revealed presence of characteristic 'pores' and 'pits' on the cell surface of pressure-treated versus untreated cancer cells. TEM analysis also revealed significant intracellular differences between pressure-treated and untreated cancer cells. Hyperbaric nitrogen therapy is proposed as a novel cancer-treatment modality involving administration of N2 at the tumor site in murine models of breast and lung cancer. This would eventually pave the way for development of a device effective treatment strategy for human tumors in future.https://doi.org/10.1371/journal.pone.0311685 |
| spellingShingle | Mohsin Ali Khan Zaw Ali Khan Ishrat Husain Shivbrat Upadhyay Sarina Zehra Rumana Ahmad Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study. PLoS ONE |
| title | Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study. |
| title_full | Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study. |
| title_fullStr | Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study. |
| title_full_unstemmed | Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study. |
| title_short | Effects of pressure on the survival and viability of cancer cells in vitro: An analytical study. |
| title_sort | effects of pressure on the survival and viability of cancer cells in vitro an analytical study |
| url | https://doi.org/10.1371/journal.pone.0311685 |
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