Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study
Abstract Introduction Fibromodulin, a small leucine rich proteoglycan has been suggested to have prominent role in wound healing. On the other hand, fibroblast cells, due to their ability to secrete growth factors and control inflammation in the wound area, have been proposed as effective approaches...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-05-01
|
| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-025-00975-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849730983446708224 |
|---|---|
| author | Negar Abdollahzadeh Mehran Vatanchian Fatemeh Oroojalian Seyed Ehsan Enderami Amir Amani Reza Salarinia |
| author_facet | Negar Abdollahzadeh Mehran Vatanchian Fatemeh Oroojalian Seyed Ehsan Enderami Amir Amani Reza Salarinia |
| author_sort | Negar Abdollahzadeh |
| collection | DOAJ |
| description | Abstract Introduction Fibromodulin, a small leucine rich proteoglycan has been suggested to have prominent role in wound healing. On the other hand, fibroblast cells, due to their ability to secrete growth factors and control inflammation in the wound area, have been proposed as effective approaches in cell therapy for wounds. In the current study we attempted to improve treatment results using a combination of fibroblast and fibromodulin features. Method Fibroblast cells were isolated from the skin and transfected with a vector carrying the fibromodulin gene. Following the assessment of fibromodulin protein production, the effect of transfected fibroblast cells was studied in an animal wound model. Results Flow cytometry analysis showed high expression of the CD90 marker (97.2%) and very low expression of the CD34 marker (0.47%). Additionally, enzyme-linked immunosorbent assay (ELISA) findings confirmed high expression of the fibromodulin gene in the transfected fibroblast cells. In vivo studies demonstrated that the animals treated with fibroblast cells transfected with fibromodulin (V + G+) exhibited significantly improved wound contraction on day 7 (i.e., contraction percentage: 21.79 ± 9.96%, compared with 7.23 ± 2.30% in the PBS-treated group). Histopathological studies also indicated improvements in angiogenesis score and collagen density score in the animals treated with the V + G + group. Conclusion The results of this study showed that fibroblast cells expressing the fibromodulin gene improve wound contraction and some histological parameters in the deep wound model of the rat. |
| format | Article |
| id | doaj-art-603c4be6c1cf4ea59b9ea47d8daa15ee |
| institution | DOAJ |
| issn | 1472-6750 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj-art-603c4be6c1cf4ea59b9ea47d8daa15ee2025-08-20T03:08:43ZengBMCBMC Biotechnology1472-67502025-05-0125111110.1186/s12896-025-00975-5Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo studyNegar Abdollahzadeh0Mehran Vatanchian1Fatemeh Oroojalian2Seyed Ehsan Enderami3Amir Amani4Reza Salarinia5Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical SciencesDepartment of Anatomical Sciences and Pathology, School of Medicine, North Khorasan University of Medical SciencesDepartment of Advanced Technologies, School of Medicine, North Khorasan University of Medical SciencesImmunogenetics Research Center, School of Medicine, Mazandaran University of Medical SciencesDepartment of Advanced Technologies, School of Medicine, North Khorasan University of Medical SciencesDepartment of Advanced Technologies, School of Medicine, North Khorasan University of Medical SciencesAbstract Introduction Fibromodulin, a small leucine rich proteoglycan has been suggested to have prominent role in wound healing. On the other hand, fibroblast cells, due to their ability to secrete growth factors and control inflammation in the wound area, have been proposed as effective approaches in cell therapy for wounds. In the current study we attempted to improve treatment results using a combination of fibroblast and fibromodulin features. Method Fibroblast cells were isolated from the skin and transfected with a vector carrying the fibromodulin gene. Following the assessment of fibromodulin protein production, the effect of transfected fibroblast cells was studied in an animal wound model. Results Flow cytometry analysis showed high expression of the CD90 marker (97.2%) and very low expression of the CD34 marker (0.47%). Additionally, enzyme-linked immunosorbent assay (ELISA) findings confirmed high expression of the fibromodulin gene in the transfected fibroblast cells. In vivo studies demonstrated that the animals treated with fibroblast cells transfected with fibromodulin (V + G+) exhibited significantly improved wound contraction on day 7 (i.e., contraction percentage: 21.79 ± 9.96%, compared with 7.23 ± 2.30% in the PBS-treated group). Histopathological studies also indicated improvements in angiogenesis score and collagen density score in the animals treated with the V + G + group. Conclusion The results of this study showed that fibroblast cells expressing the fibromodulin gene improve wound contraction and some histological parameters in the deep wound model of the rat.https://doi.org/10.1186/s12896-025-00975-5FibroblastCell isolationPrimary cultureWound healingFibromodulin |
| spellingShingle | Negar Abdollahzadeh Mehran Vatanchian Fatemeh Oroojalian Seyed Ehsan Enderami Amir Amani Reza Salarinia Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study BMC Biotechnology Fibroblast Cell isolation Primary culture Wound healing Fibromodulin |
| title | Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study |
| title_full | Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study |
| title_fullStr | Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study |
| title_full_unstemmed | Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study |
| title_short | Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study |
| title_sort | fibromodulin overexpressing fibroblast cells increase wound contraction improve scar quality and enhance angiogenesis an in vivo study |
| topic | Fibroblast Cell isolation Primary culture Wound healing Fibromodulin |
| url | https://doi.org/10.1186/s12896-025-00975-5 |
| work_keys_str_mv | AT negarabdollahzadeh fibromodulinoverexpressingfibroblastcellsincreasewoundcontractionimprovescarqualityandenhanceangiogenesisaninvivostudy AT mehranvatanchian fibromodulinoverexpressingfibroblastcellsincreasewoundcontractionimprovescarqualityandenhanceangiogenesisaninvivostudy AT fatemehoroojalian fibromodulinoverexpressingfibroblastcellsincreasewoundcontractionimprovescarqualityandenhanceangiogenesisaninvivostudy AT seyedehsanenderami fibromodulinoverexpressingfibroblastcellsincreasewoundcontractionimprovescarqualityandenhanceangiogenesisaninvivostudy AT amiramani fibromodulinoverexpressingfibroblastcellsincreasewoundcontractionimprovescarqualityandenhanceangiogenesisaninvivostudy AT rezasalarinia fibromodulinoverexpressingfibroblastcellsincreasewoundcontractionimprovescarqualityandenhanceangiogenesisaninvivostudy |